FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCACGACGGTCTAATCCCAGCTCACGTTCCCTATTAGTGGGTGAAC34.225352112676056No Hit
GCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTG34.225352112676056No Hit
GCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAA34.225352112676056No Hit
TGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAAT34.225352112676056No Hit
GTTATCCAAGTGGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGATTT22.8169014084507045No Hit
CGACCCAGAAGCAGGTCGTCTACGAATGGTTTAGCGCCAGGTTCCCCACG22.8169014084507045No Hit
GGGGGCACCTGCACCACGGCTCACCTTTCAGAGGTTCTGCACTTTTTGGG22.8169014084507045No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG22.8169014084507045No Hit
CCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCGGT22.8169014084507045No Hit
ATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGCACATAC22.8169014084507045No Hit
CTGTACCCCATATTCCCTGTCTGCCCCAAATCCTCTGGGTCAGCGGGAAT22.8169014084507045No Hit
GTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAA11.4084507042253522No Hit
TATCTAAGCTTCTCAGAACACTCTCCTAATAAGCAGATGTTTGGCCTTTC11.4084507042253522No Hit
TACCTGGGCTGATGGGATTTTTAATGTGTTCATTTTTAACTTGAAGCTGA11.4084507042253522No Hit
AGCGAAGACCAGGCTGGGCTCCCAGAGAGCCTCCCCGAAAATCACTGATG11.4084507042253522No Hit
GTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCGTTATTCCCATGACC11.4084507042253522No Hit
TTCGTGGGGAACCTGGCGCTAAAACAATCGTAGACGACCAGCATCTGGGT11.4084507042253522No Hit
CGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGA11.4084507042253522No Hit
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA11.4084507042253522No Hit
TTGTTTACACTGTGAGGGGAAAACAGCCTACTCAAGCCTCAGTAACGGTG11.4084507042253522No Hit
GCCTGAGTCACTGTCTCACAAAAAAAAAAAAAAAAGATAGACCAAAGATT11.4084507042253522No Hit
CCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATC11.4084507042253522No Hit
TAGTGACATTGGGAATGGAGAGGAGCAGATGCATAAAAGGGGTTATGGTA11.4084507042253522No Hit
CCCCTTGCCGATGCCCACCTCACCAAGAAGCTACTGGACCTCGTTCAGCA11.4084507042253522No Hit
ACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC11.4084507042253522No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG11.4084507042253522No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA11.4084507042253522Clontech Universal Primer Mix Long (95% over 21bp)
CTCAAGGAGGAAAGTCTGAACTTGTCAAACAGTCCCTTAAGAAGCCGAAG11.4084507042253522No Hit
CCTGTCAAACGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGA11.4084507042253522No Hit
GCCCGGCGGGTCATGGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTT11.4084507042253522No Hit
ACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGAT11.4084507042253522No Hit
GTCTAACCCAGCTCACGTTCCCTATTAGTGGGTGAACAATCCAACGCTTG11.4084507042253522No Hit
CAGGAGGACAAAGTCTTCATTGCGACGAGGAGCGGAAAGCTCAGAAAAGA11.4084507042253522No Hit
GTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCA11.4084507042253522No Hit
GAAGATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGG11.4084507042253522No Hit
GTAATTGTATAACTGGGAGCAAAAATATATTCTGCTTTTCAACTGTAGGT11.4084507042253522No Hit
GGTGGTGGTAGTGGTCCTCATCGTCGTGGTGATTGTGGGAGCCCTGCTCA11.4084507042253522No Hit
TGACTGGATGGGGGCATGGAGCTAGGTGGAAAGAGGAAAAGTGTCTTGAA11.4084507042253522No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC11.4084507042253522No Hit
GGCCTAGGGAGGCTGAGTGACTTGCCCGCACTCACAAAGTCAGTCAGTGG11.4084507042253522No Hit
GAGTAGAGACTGGGAGGGGAGAGCAGCAGCTGGAGGGCAGGCTGGGAGCG11.4084507042253522No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG11.4084507042253522No Hit
GAGTGATCTTACAAGTGGGGATTTTGACTCCAACAGGTCTGGCCTGGGTT11.4084507042253522No Hit
CACGCTGGGGCTGCTGTCACAGGACAATCTTAAAGGAGCTGGCAAAGTCT11.4084507042253522No Hit
GTCCTAACCACTTCGGTGGTAGGAGGAGTGGGAGAGGCTCCTTTTTCAAT11.4084507042253522No Hit
GGCTTCAAGTGTTCCTACTGTCATGACCTGCCAACATGCTGGGGTTATAG11.4084507042253522No Hit
CACCTCACAACCCACTCTTCACCCCCAAAATCACGCAGGGATGGGACTCA11.4084507042253522No Hit
GTGCTGTTTCTTTTTTTTTTTTTCTCTTGACCAGAAAAAGATTTGTTTAC11.4084507042253522No Hit
GCCTGAGTCACTGTCTCACAAAAAAAAAAAAAAAAGAGAGACCAAAGATT11.4084507042253522No Hit
CTCTACTACATCTAGGACGCCTCCGGTGAGCAGGGTCAGTGGAAGCCCCA11.4084507042253522No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT11.4084507042253522Clontech SMART CDS Primer II A (100% over 26bp)
GGCGGGCGGTCCGCCGCGAGGCGAGCCACCGCCCGTCCCCGCCCCTTGCC11.4084507042253522No Hit
GTCATCACCACTCCCTAATCTCAAGTACCCCTGTCTCTTATACACATCTC11.4084507042253522No Hit
AAAATATACAGACAGGTTGTGCAAAGAATACAATCAGTAATAAGAAATAA11.4084507042253522No Hit
TAATAATACCCATAGGGGAGCTGCATAGTTTCAATTATTAATAATAATGT11.4084507042253522No Hit
GTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGG11.4084507042253522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)