FastQCFastQC Report
Fri 10 May 2024
EGAF00003644705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64
Total Bases8.9 kbp
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTCCCCGTAACCCGTCATCCCGACAATTGCCCCTTTCAGACCAACATA34.6875No Hit
CTACAGACTCATGCCACCATGCCCAGGTAATTTTTGTATTTTTTGTAGAG23.125No Hit
GTAATGAAAAACCAATATGATAAAAACAAAAATCCTCCAGTAAAGAAGGA23.125No Hit
AGGCCGAGCACAGCCGGGGTGGGCATGTGCGTGGGACACCCTGGCTCTGA23.125No Hit
TTACGTGGTTGCCCTGGGAGCCCACACCGGGGAGGAGAGCGTGGCTGACC23.125No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT23.125Clontech Universal Primer Mix Long (96% over 26bp)
CTTACCGAGAGAACCTGCGGATCGCGCTCCGCTACTACAACCAGAGCGAG23.125No Hit
GTTTAACATTTATTCACATTTATGGGCCACTTGTAAGTCTTCTATTGAGA11.5625No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTGGGTTTTTAACACATG11.5625No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11.5625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTGATTTCCTGTGTGTGTTGCCCTGGCATTAAGGGCATTTTACCCT11.5625No Hit
GTACTTTTTTTTTTTTTTTTTTGCGTTTATACCACTGCTTCCCATGTACC11.5625No Hit
ACATGGGAAGCACTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.5625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG11.5625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTACCC11.5625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTT11.5625No Hit
CACGGGCTGAGATCAAGAAATTCAAACATGTCCAAAATCATGAGATCAAA11.5625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACC11.5625No Hit
GTCAATCCTGTCCGTGTCCGGGCCGGGTGAGGTTTCCCGTGTTGGGTCAA11.5625No Hit
GCTCTGTCACCCAGGCTGGAGTGTAGTGGGGCAATCTCAGCTCAATGCAA11.5625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGAT11.5625No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GAGAGTTTATTTAAGGAAATTACCAAGGACCCACAGGCAATAAAAGCCAG11.5625No Hit
ATTATGATTATTTCATAAGTTAGACTTGCCCTACCACCCCACATACAAAA11.5625No Hit
TGTCAGGGCAGGGCCAATATCATAAGCCTTCATAAATCTCACCAAACATA11.5625No Hit
CAATCTATCCCTGGAAATACATGTTTAAACAATAGATGTCCCCAGACAAA11.5625No Hit
CACCACTGATCCCACAGAAATACAAACTACCATCAGAGAGTACTATAACT11.5625No Hit
CCTAGGTAGTAAGAGGACTGACTATACCCTGGCTGATCTGTGGGCAATTG11.5625No Hit
GACTGAAACACCCGCCCATCTTGCTGTTCTGCCTAGGCTGGAAGACCTGG11.5625No Hit
TCCCAGGCCAGCCCAGGGCCCGAAGCCCACCCACTCGCGTCTCTAGCAGC11.5625No Hit
GTTAGGACCTTTGTCCCACCTCCGCCTGGTGGACCGGCAGGGACCTGGTC11.5625No Hit
GTATAAACTAGTAGAGGTGTTCAGGAAGAGTAAACATATTTGTTTGTCAG11.5625No Hit
CCTCAGAAATGGACTAGGAATAGAAAATTCCTGCTTTCTCAGTTACATGT11.5625No Hit
GTCTTGAAAAGATTCATAATCACTCCAAATGAAATGCTCCTCCCTTGGCC11.5625No Hit
GGACCTGCAGGAACGGAGCTGGCTGGTACCAGCCCGAGACCCAGGAGGTT11.5625No Hit
CCACCACGAGGTCAGGCGATGTGGTCTACACCGGCAGAAAGGAGAACATC11.5625No Hit
GTTGAACATTTATTCACATTTATGGGCCACTTGTATGTCTTCTTTTGAGA11.5625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACAA11.5625No Hit
GCTACGAGCAGATGGCTTCACGAAAATTCAGCCTGACGACCCCGCAAAGC11.5625No Hit
CCCTTTGGGCAGCTCACCGACGAGCAGGTCATCGAGAACGCGGGGGAGTT11.5625No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGCCCATCGTGATCTCGTATG11.5625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTT11.5625No Hit
GTACTGGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTA11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAAAG11.5625No Hit
TTCTCTGCATCCTTTTTCTGGTCAATCTCTAGCTGCAGCCTCTCTGCCTC11.5625No Hit
ACCAGTGGGTGTCAGAAGAACACGAAGAGGCACATCACACTGCTTATCTT11.5625No Hit
GAAGCAGTGGTATCAACGCAGAGTACTCCTGTCTCTTATACACATCTCCG11.5625Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.5625No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.5625Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.5625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCATTTGATGATTCATTGGCAGTATTTTGTGATGACATCATATTAGTGGT11.5625No Hit
ACCTCAACCTCCCAGGCAGGCTCAAGCGATCCTCCCACCTCAGCCTTCTG11.5625No Hit
GACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAATCAAGAACGAA11.5625No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)