FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA35.172413793103448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCTCAGCATGGTGGCTCACACCTGCAACCACGGCACTTTGGGAGGCCAA23.4482758620689653No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG23.4482758620689653Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGC23.4482758620689653No Hit
TGAGTATTCTTGTTGCTCCACATCTTCACCAGCATTTGATGTTGTCAGTG23.4482758620689653No Hit
TCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGA23.4482758620689653No Hit
GTTCTAAAAACCATATAATCTGGCCATTCATGCCATAACCATAAAGGCTA23.4482758620689653No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTT11.7241379310344827No Hit
GTATACAAGTGTGTTATCGGCTGGGTGCGGTGGCTCACACCTGTAATCCC11.7241379310344827No Hit
GCCTCCTGAGTAGCTGGGACTACAGGCACCCATCACCACGCCCAGCTAAT11.7241379310344827No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCACGTACTCTGCGT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA11.7241379310344827No Hit
GGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGG11.7241379310344827No Hit
CCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGTGGGGATTACAGGC11.7241379310344827No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTATACAC11.7241379310344827Clontech SMART CDS Primer II A (96% over 26bp)
GAATGATAAGTGCAAACTTAAACCTACAAGATCGAGGAGGTGAAGTTTAA11.7241379310344827No Hit
TCCCTGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA11.7241379310344827Clontech SMART CDS Primer II A (96% over 26bp)
CCTTGAGCTATGATTGTCGTAATTTTCCCTTTCCTGCATCTCATTTGGTG11.7241379310344827No Hit
TGCCAAGCCTGTACCGCTGCTCACGCGCCAGCATCGGCCGTTCTACGGTA11.7241379310344827No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA11.7241379310344827No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGATACCACTGGTTCCCA11.7241379310344827No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAA11.7241379310344827No Hit
AATCTACTGACTCCTTTTTTTTAAGAAAAATTCAAGACTCAACTGTTAAG11.7241379310344827No Hit
CGGTCGTTGACGATCCGCGTTGAAGAACACGAAGAAGGCCCGTCAGCGGT11.7241379310344827No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCG11.7241379310344827No Hit
TCCTTCAGACGCTCGCGCATTTCCTGAATGCGGGGGTTGATGGCCTTCAT11.7241379310344827No Hit
GAATACATGTGTCAACTCAGGCAGATAGCACAAACTGCTATTAGGCATGA11.7241379310344827No Hit
CTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGG11.7241379310344827No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTAAGACAGGATCTCGTATG11.7241379310344827No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.7241379310344827Clontech Universal Primer Mix Long (95% over 21bp)
AATCAAGATCAAGCGAGCTTTTGCCCTTCTGCTCCACGGGAGGTTTCTGT11.7241379310344827No Hit
GATTTATCCTGCTGGCTTTGGGGTTGGTCTTGTTTTTCTAGTTCATCATG11.7241379310344827No Hit
GCAAACTATGATAAACTTACACTTGATGTTTGGACTGATGGAAGCACTGG11.7241379310344827No Hit
GGAAGCGGTGCAGCGTTGCGCCGAGCTGACCCCGGACCTGATCCTGATGG11.7241379310344827No Hit
CCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGCAAACAG11.7241379310344827No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CATTACCATTTTACTGTGAAATGTATTGCTTGAAATACACACACAAAAAA11.7241379310344827No Hit
CGTCGCGGCGGATCGGTGCGTGGGGGATGGGTCACTGCCCCGACGACCGA11.7241379310344827No Hit
ATGGGGGTATGATCTCATTTTTGTTGGCATGACTGGAGGGCAGGGCTGTA11.7241379310344827No Hit
CAGAGGTATCGAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATGCGTAA11.7241379310344827No Hit
TTTTTCTAGTTATAAAACTCATGTTCTTTTCTAAAAGCTTAGAAAATATC11.7241379310344827No Hit
GCAGTCCTGCATCCAGCAAGTCTGTTGGCACCATTTTCCCAACAGCAAGT11.7241379310344827No Hit
GTTTTCAATATGTTCCAGACATTATAAATGTCTTCCCTTCTATTTCCATG11.7241379310344827No Hit
CATGAACAACCATGTCGGCTACAACCATCACCTTAGCGACATCCGCAATC11.7241379310344827No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC11.7241379310344827No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.7241379310344827No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GCAGCGAAGCACGAGGCCGAAGCCATCGCCGGAAACAAGGCAAATTGGGA11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)