FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT46.896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTGCCTTAGCCTCCCGAGTAGCTGGGACTACAGGCGTGTGCCACCCT23.4482758620689653No Hit
CCCCCCGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGAT23.4482758620689653No Hit
CTACACACCTAGTAGAATGGCCAAAATCCAGAACACTGACAACATCAAAT23.4482758620689653No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT23.4482758620689653No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAGAACATGTGAAGA11.7241379310344827Clontech Universal Primer Mix Long (95% over 22bp)
GTCTGGAACTGGTCAAGGACTATGGCTGGCTGACGATTCTGGCCAAGCCG11.7241379310344827No Hit
CGCCACGATCACCACCGCGGAAATCGCCGCGATCACCACCACGGAAATCA11.7241379310344827No Hit
AGTCTGGAAGGCAGGAAGCAGAAGGAACAAAGAAAAACAAGGGACAAGAA11.7241379310344827No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC11.7241379310344827No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATATATGTCTGAAGAACAGGAAAATAGGTAATGATGTTCTTTATCCTG11.7241379310344827No Hit
GATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTC11.7241379310344827No Hit
GGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAAC11.7241379310344827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GAAAAGACTCCAGGAATCTTGTCAGTTCTAACTCTGTGTTCTTATTTTAC11.7241379310344827No Hit
GCTTTTACCCAAAGTAGTGGAAGATCGTGGTGTGCAAAGAACAGACGTCG11.7241379310344827No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.7241379310344827No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.7241379310344827No Hit
CTTTCATGACGAGGGTCCCCTGGGACGAGGGAAAGGAATGGAAAGCGGTC11.7241379310344827No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.7241379310344827Clontech Universal Primer Mix Long (95% over 22bp)
CCCATACCGAAAGCGATATGCCCGGCGATATTGTCTGCCAGCAGCGGCGT11.7241379310344827No Hit
AGTCTGGAAGGCAGGAAGCAGAAGGAACAAAGTAAAACAAGGGACAAGAA11.7241379310344827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCAGTAGCAGATGCCGTTGTCGTCCTCGGCGACGAAGTAGTCGCGTGTC11.7241379310344827No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAATAAACCAATAAATGACTAATTAACAACAATAAAAAATCATAAATAAT11.7241379310344827No Hit
GTATTAAGAGTACTTAATATTCTCCAAATCTTCTTAGCATTCTGCAAAGA11.7241379310344827No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACAC11.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATATGGTTTTGAAATAGCTAGAGGCTCGCTAAAATTGTTCCAGTTGCTC11.7241379310344827No Hit
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GATCGCACCAAGACGCGCCCAGGAGATCGTGTCGATCCCGATGTGGCACT11.7241379310344827No Hit
CATCCTTGGGGTTGCCCACGCCCAGTGCCGGGGTGTCCACCACATCCAGG11.7241379310344827No Hit
CTCCTTTGAATCAGTGCAGACAGACAGAAATAAAAGAGATAGAATTTTAA11.7241379310344827No Hit
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT11.7241379310344827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT11.7241379310344827No Hit
TAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGG11.7241379310344827No Hit
GTTTCACTATGTTGGCTGGGCTGGTCTCAAACTCCTGACCTCAAGTGATC11.7241379310344827No Hit
TGATTTCAGCATGTTGAGCTTCGTCAGTTAAACCAGCGAATATATTAAGG11.7241379310344827No Hit
TATTTTGGGCCTTCATGGGCAACTCAGTCAGGAAGCTTCAGGGAAAAAGA11.7241379310344827No Hit
ATCCCAGCACTTTGGGAGGCCAAGGAGGGCAGATCAAGCCAACATGGTGA11.7241379310344827No Hit
TATTTGGGCACAGTTCGTGGCACCCCAAAACAATTACAATAGTGACACCA11.7241379310344827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATATAACCAATCT11.7241379310344827No Hit
GCATTGGTCAGGGCAATCTTGACTCAGTCTATCAATTCAAATGCTAATCT11.7241379310344827No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA11.7241379310344827Clontech Universal Primer Mix Long (95% over 21bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC11.7241379310344827Clontech Universal Primer Mix Long (96% over 26bp)
CGCTGAGGCCGTGCACGGTCCCTGACAGGCTGCCTCCCTCTCCTGCTCTT11.7241379310344827No Hit
CGAGAATACCTGGAGAATTGGGCTCAAAAGTAAAGGGTTTAGAAACTGTC11.7241379310344827No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)