FastQCFastQC Report
Tue 10 May 2022
EGAF00003644722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595947
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.16981375860605055No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6480.10873450155802446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTAGACTGATACCTCCTCATGAAATTTCTGCAGACGTCGCGCCATCG6360.1067208996773203No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6110.10252589575918664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)