FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2113.125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT127.5Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG63.75Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG63.75Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42.5Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT42.5Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG42.5Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA42.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA31.875Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC31.875Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.875Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG31.875Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT31.875Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA31.875Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.25Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.25Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21.25Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG21.25Clontech SMART CDS Primer II A (96% over 26bp)
GTACAGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21.25Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA21.25Clontech Universal Primer Mix Long (95% over 22bp)
ACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA21.25Clontech Universal Primer Mix Long (96% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.25Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGTGTAGCCGCTGGTGCCGCCGGCCGGGTTCAGCGCCACGCCCGCGC21.25No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21.25Clontech Universal Primer Mix Long (96% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC21.25Clontech Universal Primer Mix Long (96% over 25bp)
CAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTA10.625No Hit
ACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.625Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGT10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.625Clontech Universal Primer Mix Long (100% over 23bp)
AACGCAGAGTACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.625Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.625Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGTATC10.625Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTA10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATGTATCCTGTGGGCAATTATTTGCACACATCTGTTGAAGTGTTTGGTT10.625No Hit
GCTTCCCATGTACTCTGCGTTCATGGGAAGCAGTGGTATCAACGCAGAGT10.625Clontech Universal Primer Mix Long (100% over 23bp)
GCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC10.625Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATTCTCATCTTCGAATAT10.625Clontech SMART CDS Primer II A (96% over 26bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.625Clontech Universal Primer Mix Long (96% over 26bp)
CTTTGAATCTGGCCATCAGAAAGAAAAATGCGCTCCTCCGGAGCAAGTGA10.625No Hit
CCCTATTGTTGATATGGACTCTAGAATAGGATTGCGCTGTTATCCCTAGG10.625No Hit
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.625Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.625Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAGCA10.625Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.625Clontech Universal Primer Mix Long (96% over 25bp)
TTTGCAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.625Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTATA10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTACAACGCAGAGTACTTTT10.625No Hit
TACCCGAACAGTCGCGGGCGCTGCGCGATGACCATGCGCCGCAGTTCTTG10.625No Hit
GAGTACATGGGAAGCAGTGGTATCAACCATGCTTCCCATGTACTCTGGGT10.625No Hit
CCCATGTACTCTGCGTTGATACCACTGCGTCCAAGTAATCTGCGTTGCAA10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCTG10.625Clontech Universal Primer Mix Long (95% over 21bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.625Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGTTGAT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCAGCCATCGTCGGGACCGTCGTCGCGCTCTTCGCGGCGCGCCCTTG10.625No Hit
GTACATGGGACGCAGTGGTATCATCGCTGAGTACATGGGAAGCAGTGGTA10.625No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.625Clontech SMART CDS Primer II A (96% over 26bp)
TTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTTCTTACCAT10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGT10.625Clontech Universal Primer Mix Long (96% over 26bp)
CTCAGGGCGCAGATCCTGGAGCGCAACAGCGCGTTGCAGCGCGCCAACAC10.625No Hit
GTATCAACGCAGAGTACATGGGAAGTAGTGGTATCAACGCAGAGTACATG10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.625Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCGACGCAAAAAAAAAAAAAAAAAAAAAAA10.625No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.625Clontech Universal Primer Mix Long (96% over 26bp)
TCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGAACTCTGCGT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAATTTA10.625No Hit
GGAAGCAGTGGTATCAACGCAAAGTACATGGGAAGCAGTGGTATCAACGC10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAACGATATGTA10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAACATAGCTGGATGCCGCGCCTGCCCGATTTCGATCATCTTCTGGTG10.625No Hit
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGTTGAA10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC10.625No Hit
CTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.625Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCATTTGTATA10.625Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTACATGGAAAGCAGTGGTATCAACGCAGAGTACATGGA10.625Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCATGTC10.625Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCTCTGCGTTGATACCACTGCTT10.625Clontech Universal Primer Mix Long (95% over 23bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10.625Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph