FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3018.75Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1610.0Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC53.125Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT53.125Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA42.5Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA42.5Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC42.5Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG31.875Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC31.875Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC31.875Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG21.25Clontech Universal Primer Mix Long (96% over 26bp)
GTTCGCCACCGGCTCCATCAAGCACACGCTGCTCGCGGGGCTTGAGTTGG21.25No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.25Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC21.25Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.25Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA21.25Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG21.25Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGAATACCACTGCTTCCCATGTACTCTGCGTTGA21.25No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.25Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC21.25Clontech Universal Primer Mix Long (96% over 25bp)
CCATGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC21.25Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.625Clontech Universal Primer Mix Long (100% over 23bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.625Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCGGGTGGTATCGGCCCTCCGTCAGTGGCGATTCATGCGCTCCCGTTTG10.625No Hit
GAAGCTGATCGGCACGACCATCCCCGAAGTCGTGATCGAGGGCGTCGAGG10.625No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATA10.625Clontech Universal Primer Mix Long (96% over 27bp)
GGCAACATGGCAAAACCCCATCTCAACAGAAAATACAAAAATTAGCTGGG10.625No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.625Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.625Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.625No Hit
ACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCT10.625Clontech SMART CDS Primer II A (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAAACTGGA10.625No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.625Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGATTCCCATGTACTCTGCGTTGAT10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACAGTGCGATT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCATAGTACATGGGAAGCAGTGG10.625Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGAAGCAGTGGTATCAACGCAACTGCTTCCCATGTACTCTGCGTTGATAC10.625Clontech Universal Primer Mix Long (95% over 21bp)
GACCTCGGAGCAGAACCCAACCTCCGAGCAGTACATGCTAAGACTTCACC10.625No Hit
CCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTC10.625Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGATGACTCT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGCAGAAGCTTAAACAACGCT10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.625No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC10.625No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAAAAAATGA10.625No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.625Clontech Universal Primer Mix Long (95% over 21bp)
GCTTTCCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGT10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.625No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATACCACTG10.625No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCAAGTACTCTGCGTT10.625Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGC10.625Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTACCA10.625Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGAGGAATCAACTCAGAGTAAAGC10.625No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGGTCGGTTTAAAC10.625No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGAGCAGTGGTATCAACGCAGA10.625Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCATTTC10.625Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCATAGTACATGGGAAGCAGTGGTATCAACGCAGATTAAATGT10.625Clontech Universal Primer Mix Long (95% over 24bp)
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAACGTTTTAACAAA10.625No Hit
CAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.625Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGC10.625Clontech SMART CDS Primer II A (100% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.625Clontech SMART CDS Primer II A (96% over 26bp)
CCTGAAGCCGCCGCCCCCGCGGCGGTGTTGGCGCGCTGCAACGCGCTGTT10.625No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.625Clontech Universal Primer Mix Long (96% over 26bp)
CATGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.625Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC10.625Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAA10.625No Hit
TATCCGAACAACCCGACGGGTAATCTGTTCGACGCGGCGGACATGGACGC10.625No Hit
CTCTACGCTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC10.625Clontech SMART CDS Primer II A (95% over 24bp)
CCCCTGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.625Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTCGTTAA10.625No Hit
TATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.625Clontech Universal Primer Mix Long (96% over 26bp)
CTTCGATACGATGCGCTTCGGATCGTGGATCTCGTTGATCAGCAGGTTGC10.625No Hit
ATGTTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.625Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph