FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003644820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGAGCACGGCCTCGGCGATCGCCGGATTGCCCCGGTAGCCGTTGAAGA212.5No Hit
CAATGCAAACTGACAAGGTGCTGTTTGAACTGTCGGACGGAAACCGTGTG16.25No Hit
GTACAATCGCACCTCGTTTCGCACCAGTACGAATCCCTGATGAAGAAAGC16.25No Hit
TCATATAACTGATACGGGGCAGAGCTACGACTCAATCCAGTTTCTTTTGA16.25No Hit
CTTTAACGTCAACACGCAGGAGCTGCGGCGCGTCACGGTGCCGGACGGCC16.25No Hit
GCTTAAGCTGCTGCGCCTCATCGGCAGCAACGCCGAGATCGGCGAAAATA16.25No Hit
ATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCGAA16.25No Hit
GCCCAGATGTAGGGTCCGCAGCCTCGATGCTGTGCTTTCTACCTGCCGAG16.25No Hit
GAACCTCCCGGGGGCTTTGTAAGCTGAGACTCCATTGTACACCTGGTTAC16.25No Hit
AGGTTAGGTAGTGCTTAGAAGGCAGTTCCAAGCACACAGCTACTATTCTT16.25No Hit
ACGTGAAATTTCACGTGCACCAAAACTGCGTGAAAATCACGTGAAGTTGG16.25No Hit
CGGTAACGGACACCGTGAAGACAATACCGACCGCGACAACCCACATCGGT16.25No Hit
CCAGCGACATGGGGCATATCCCCCAAAGCATGGACAGCGTCATCTTTACC16.25No Hit
CAATAACGTCATAGTCGTTCTCGTGTCCCTTAAACCTGTCTCTTATACAC16.25No Hit
GATAAAGACTTGCATCGCGCTTGTCTTCTGGCGTAGCATCTACAACTATT16.25No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)