FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTGAGTGCTACCTAGACTTACCATTAATGAACAGCCTTGGCAAGGCTGA43.278688524590164No Hit
CAGAAAACAGAAGAAAAAGCAGGGGTTGCTTTCTAATTTCAGACAAAGCA32.459016393442623No Hit
ATTTATTGGTGAGTATTAAGAGGGGGGCAGCACATTGGAAGCCCTCATGA32.459016393442623No Hit
CTGGAGGTCCCGGCAGGCACAGGGCTCACACCTTGGGTTTTGCAAACACC32.459016393442623No Hit
AAGTACATCGACAAGACGATCCGGGTAAAGTTCCAGGGAGGCCGCGAAGC21.639344262295082No Hit
CCCCTACAGGGCCACCTTGGGGTCCACTTCCCCGGGTCCCTGGCCTTTGG21.639344262295082No Hit
GGTCTGACACGCTGCGGTTGACATTGACAGACATTTTGGCTTATTAGTGG21.639344262295082No Hit
GCTCAAAATGGAGTTGCCGGAGAGACAGCCCAGGTTCGTGGTTTACAGCT21.639344262295082No Hit
GATTTTTTCCCCAATCATATAGAAATACAAAAATCATTCTTAGCTCATGG21.639344262295082No Hit
GTTCATATTGTCACACTCCACATTTTCAAGAGGTGCATCCTCAGGCAGAT21.639344262295082No Hit
CCATATGACAGTAACTAGCTTAAAAGTCTAAAGACTTGCTCTCAAGATGG21.639344262295082No Hit
GTCTAAGGCTCTACTTAAGTGTGGACTTTCAGGAGCTAAATGATGATAGA21.639344262295082No Hit
AGTCTGCAGGTGCGGCCAAGCGCAAAGCGTCTGGGCCCCCGGTGTCCGAG21.639344262295082No Hit
GTCGCAGGAACTGCTCGCCAAACCGCTGTCGCAGAGTGATGCGGTGCAGC21.639344262295082No Hit
CGGTATAACTGTGTTCCTCTGAAATACCATGGCAAACAACCTAAGCCTTT21.639344262295082No Hit
GTGGTATCAACGCAGAGTACATGGGAAAAAATAAATAAATGTAGAAAAAT21.639344262295082No Hit
ATTTAACTTATACTTAGTTATAAATAAAGTAATATCATAACATCTGAAAT21.639344262295082No Hit
CTGCCAGCCAGATAGATAGACAGATGGGAAAGGCAGGCGCGGCCTTCGTT21.639344262295082No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGA21.639344262295082No Hit
GCCTTTATGGAAGGGTATCCGCCAACACAGAGGAAAGGGTTTGGCTGCTT21.639344262295082No Hit
GAAGATATCTGTTAGCTAGAAAGAAGATATGAATGGTTCTTTGGTATGTA21.639344262295082No Hit
ATATTAAGCTATAGTTGTCTTTCTGGGGCTATCTCTATTGGCTTAGAGAT21.639344262295082No Hit
GCCCCAGGATCCGGCTGCCAGCCCTGAGGCCAAGCACGGCTGGAGACCCA10.819672131147541No Hit
AATATAGGGTGTCAACAAAATTTCAACAAATTGAGTCTCAGAGATCTGAT10.819672131147541No Hit
ACTCTGGGGAGACTGGGAAGGTCCTCAGCCATTCAGCACCATGCGGACGA10.819672131147541No Hit
GGAATGGAATGGAATCAACTCGATTTCAATGGAATGGAAAGGAATGGAAT10.819672131147541No Hit
CCCAGGGACTTTATTTAAATAATGAAAATGGTTATGTTATGAATGGCCCT10.819672131147541No Hit
TGTATGTACTATGTGGTATGGAAACTTTTTTAAAACAATTAAAGGTGAAT10.819672131147541No Hit
GGCATGGACCCGGTGCGCAGCCAGCGCATGCTCAAGATCTGTCCGCACGA10.819672131147541No Hit
GTCTCCATCCAAGCTGCGCAGGCTCCAGCAGGCCGCAGGCTCGCAGGCGA10.819672131147541No Hit
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCACCGCCACGGGG10.819672131147541No Hit
CTAAGGAGATGAGGAGCTCTTTATAGATATCACGAGTGTACTTGGTAGAG10.819672131147541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTAAATGAAG10.819672131147541No Hit
GCAGAGTACATGGGAGGGTTCTCGCTCTTGTCGTGTCTGTTCAAACCGGC10.819672131147541No Hit
GGTATCAATCAGTAAGGAAAACAAATAGAAAAAGAGGCATAATCTGGTAT10.819672131147541No Hit
CTTTGTTGACTGTGTACCTTGGTTTTTTGTTTTTAAATTGCATTTTTATT10.819672131147541No Hit
GCTCTGGTGCAGTCTCCGCCTGTCAGTGCCGGCAGTCGTCCGCGGCGCCC10.819672131147541No Hit
TATCTAGAGTCACCAAAGCCGCCGGCACCCGCCCCCCGGCCGGGGCCGGA10.819672131147541No Hit
GTGTAGCCGCTTCTCTATACGGGATTCCAGCTCATCGGCTATCGTTAGTG10.819672131147541No Hit
ACCGAGGACGGGGACTCGTTCCTGCACTTGGCCATCATCCATGAAGAAAA10.819672131147541No Hit
TATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGC10.819672131147541No Hit
AATTAAGGGTTTGATGCTTTTCTTCTAATACTTTTCCTGAAACATCTGGA10.819672131147541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTCGATACGG10.819672131147541No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAA10.819672131147541No Hit
GCCCTGGGCAGCAGGAGGAAGGCCAGCAGAAGCAGGATTGGTTGCATCTT10.819672131147541No Hit
GGTTGGCGACGTGACCGGGGCCCAGGCCTATGCCTCCACCGCCAAGTGCC10.819672131147541No Hit
CTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTG10.819672131147541No Hit
GCTTCTGTTCCATGTGCTTCTAGCTAGCATGACCATGTGGGGCTGGGCAG10.819672131147541No Hit
CAAGAAGAAAGGGGCAGCTGCCGCCACCTCCTCCAAGACACCTTCCTGAG10.819672131147541No Hit
TCCTCCTCTTCCTCCCCACCTTCTTCCTCTTCTTCGTCTACCTCATTGTC10.819672131147541No Hit
CTTCTTGAACTGGTGCTGTCTGGGCTTCATAGCATTCGCCTACTCCGTGA10.819672131147541No Hit
GAACAACAGATGATGTATGCAGGGAGTAAAAACAGGCTGGTGCAGACAGC10.819672131147541No Hit
GTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGA10.819672131147541No Hit
AGATAGAATCGTTCTCAGAAAGTGCTTAGTGACGTGTGCGTTCAATTCAC10.819672131147541No Hit
GTCAGAGTGGAGCTGTCCCCCCGACGAGCCCCCAGAAGCACCCGGCCGGC10.819672131147541No Hit
GTTCAATCGTTTCTTTGGAAGGCAGTGGATTTTTTTGGTTGGGGTGGGTG10.819672131147541No Hit
ATCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAAT10.819672131147541No Hit
GTACAGAGCAAGAGGATAGCTGAGACCCAGCCCAGGTAGAAGGACCAGGA10.819672131147541No Hit
GAACAAACATGCATATATGGCTACAGATTTTCCAAGAGTTGGATTAACCC10.819672131147541No Hit
CACGTAGGCGCTGAGCGTGAGCACGAAAACGAGGATGGTGCCGGCCTGTA10.819672131147541No Hit
CAGTTATTTATTGCTGAGTGAGCAAACCCCTAGCCCCCAAGTGGGGACTA10.819672131147541No Hit
TGGCGAGAGTTTGTGCGGCGACATGAAACTGCTTACCCACAATCTGCTGA10.819672131147541No Hit
ATTATAGGCCAGTGATGTCTCAAAGAGTAGAGGAGCGTCTACTGGTCTTT10.819672131147541No Hit
GAGTCCCACAGAGGAGGCTGTTGGAAATAACATCATCTTGTAAACTTTTT10.819672131147541No Hit
TGCACGACCTCGGGCTGCCACTGCGGATCGAGGCCCTGCGCCAGCTGCGC10.819672131147541No Hit
CTGCAACCTCTGCCTTTCGGGTAAAAGCCGTTCTCCTGCTTCAGCCTCCT10.819672131147541No Hit
GTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCA10.819672131147541No Hit
GGTTATGTGGTCCGAATCAGTGGTGGGAACGACAAACAAGGTTTCCCCAT10.819672131147541No Hit
ACCTCACCCTCCCAGGTTGAAGTGATTCTCCTGTCTCAGCCTCCTGAGTA10.819672131147541No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT10.819672131147541No Hit
GTACATGGGGAGCGGAGCAGAGCCGAGGTCGGGCCGCGAGCGGAGCCGGC10.819672131147541No Hit
GCTGTGATGGGGAGAGGAAATTGTTTCCTCGAGTCGTTGATTTCACACTG10.819672131147541No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA10.819672131147541No Hit
GATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCT10.819672131147541No Hit
ACCACAGACTGAGAGAATGCAGAAGAGGGCTGAACGATTCAATGTACCTG10.819672131147541No Hit
GAATGTACGGTGATATTGGCGGACTTAAGGACATCGAATTGTTTATCAGG10.819672131147541No Hit
CCCTATGGGATTTGTCATTGGATGTTATTTAGACAGAAAGAGTGATGAAC10.819672131147541No Hit
AAATAAAATTTGTTAAATCAGACAAAATAGTAGGGACTGAAAACTAGTGT10.819672131147541No Hit
CCTCATTGGTGGGGCTCCTGACGCCCCAAGAGGCGGACGTGACTTGCCTA10.819672131147541No Hit
GTACGAGACCGGCGAGCATGCCGGCCGGCTGCTCTTCGACATGCTCGCCG10.819672131147541No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT10.819672131147541No Hit
GCACAAACGGCGGCGTATTGCTCTGAAGAAGCAGCGTACCAAGAAAAATA10.819672131147541No Hit
CACTCGGCCATCGTCATGCACGTACTTGTAGCTGTAAACCACGAACCTGG10.819672131147541No Hit
ACTTAAGAGGATTGGCAAACCGGAGAAACGCTGGGAGAGGTGCCCCGCAC10.819672131147541No Hit
TCTTTTACCTCACATTGCATTTTCTCTTGGCAATGTTTGGGTTTGGGTAC10.819672131147541No Hit
GCCTCGGTCTCCTGAGTAGCTGAGATTACAGGTACGCTCCACCACGCCCG10.819672131147541No Hit
CATGGCCTCGAGGTCAGACATGCACATCAGAAAGCAGAAGCAGTGCTTTC10.819672131147541No Hit
GGGCCGCTGAGGCGGCAGTAGCGACCTCCGGAAGCGAAGACGGGCAAGGC10.819672131147541No Hit
CAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAG10.819672131147541No Hit
GCGCCTAGTCAACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATA10.819672131147541No Hit
ATGATTGACAGTTCACACTGGAATCAAAGGTGAATTCATGGGAGTAGAGG10.819672131147541No Hit
CCATACTCCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACA10.819672131147541No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.819672131147541Clontech SMART CDS Primer II A (100% over 26bp)
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT10.819672131147541No Hit
CGCGCAGATCGCGCCCTTCGATGTGGCGGCAAAGCAGGGGTTGCTCGAAG10.819672131147541No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)