FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences122
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGAACCTAGTCATGTGTCATCCCTAACTCTCTAAGCAAGTCAAAGAAC43.278688524590164No Hit
GAAGTCTCCCACCATTATTATGTAAGTTTCTTTCTAGGTTCCTAAGAACT32.459016393442623No Hit
TCTCAAAGAAGTGGGGCTTCACCAAGTTCAATGCTGATGAATTTGAAGAC32.459016393442623No Hit
GGAGTGGCTAAGGTTGGACAATACACGTTCACTGCAGCTGCTGTCGGGGC32.459016393442623No Hit
CAAATTTGGCTTTGTGTATTAGCATTGTGCGTGTATGTAAAAACACCGAC21.639344262295082No Hit
TCCAAGTCCCCAGTCACCTTGAGGACTCAGACATCAGGAATTCAGTCCCC21.639344262295082No Hit
GAACTGGAGCTTAGAAATATTAGTTGGAGACAAATATTTAGAAAGGATCC21.639344262295082No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT21.639344262295082No Hit
CATTGGTACCTGTATTGGGGAAACATAGCATACAAGCAAGAAGCTTACAG21.639344262295082No Hit
TATCAACGCAGAGTACATGGGAACTCGGTGGTGGCCACTGCGCAGACCAG21.639344262295082No Hit
GACTACGGCTTCCCCGAACGCAAGGAGCGCGAGATGGTGGCCACACAGCA21.639344262295082No Hit
GTGTGGGGGGAGGGAGTGAGTTGATTGACCAGATTATCAAATTATCATTT21.639344262295082No Hit
GCGCAAGCTGCACCGCATCACTCTGCGACAGCGGTTTGGCGAGCAGTTCC21.639344262295082No Hit
GGCCTTATGAGTGTGGCCAGTGTGGAAAGTCCTTTAGCCAAAAGTCTGGT21.639344262295082No Hit
GATGTCTTAATGCAGTCCAAACAGAATGGAAACAAACTTAGAAAGGATTT21.639344262295082No Hit
GTTTTGGGGATTCAATTTGAGTAAGTTTGCTATGTATTAATATATGATAA21.639344262295082No Hit
ACCAGATACAGACTACAGAGCTGGTTCTTCGTTTGAACTCTCTCCATCTG21.639344262295082No Hit
CTGGTGTTAAGTCGAAATACGGTTCGTGTAGTAGAAATTGCACGGGACTA21.639344262295082No Hit
GCCTTCTTGTTGAGTTTGAAGGAACCCGACGCGCCGGTGCCCTTGGTCTG21.639344262295082No Hit
GTTATGGTTCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGT10.819672131147541No Hit
GGAATGTACAGTGCATATTGGCGGCGCACGCCTCATTACGATTCGCCTGC10.819672131147541No Hit
GGTAGACCCAGAGCTAACAGAAAAGCTGAGGAAATTCCGCTTCCGAAAAG10.819672131147541No Hit
GGGAAAATATGATGTAAATTCAGACAAAAGTAGGAGAGAGAGAATGTTGA10.819672131147541No Hit
GTGCGCAGCTTCGGCTGGCTGATCCTGCTGTCGGGCAACGGTGTCATCAA10.819672131147541No Hit
GGCTAGGCGTCCTGCTGCTGGATGAAGGGGTTGGGGATGGCCTCCATGCC10.819672131147541No Hit
GGACGCGCGCGTGCTGCTGCCGGGCTTCCCGGCCATCGTGGCCGGCGTGC10.819672131147541No Hit
GGACCAAGGGGGCAGGGGCAACAGCCCCAGCGTGCAGGGCCAGCATTGCA10.819672131147541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTACAAAAAAGGA10.819672131147541No Hit
ATATATATGCATACACAAGCAAAGCCAGAAGAGAATAAATAGCAAATGAA10.819672131147541No Hit
AAATAAATGACTTCTTAAGAAAATATGATTTGCTAAAATAGATCCAAATA10.819672131147541No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCGCATTCC10.819672131147541No Hit
GGTCTGATCCGGAAATATGGCCTCAATATGTGCCGCCAGTGTTTCCGGAT10.819672131147541No Hit
AAGCAGATTCTGAGAATTATTCTGTCTAGTTTTTATAGGAAGATGTTTCT10.819672131147541No Hit
TTGATGGACAGTTCAGAGGCACTCACAAACTCAGAATGCAGCTTTGGTTT10.819672131147541No Hit
CTCCCGCGTGGGCTTCTCTAATTCCATTGTTTTTTTTAGATTCTCTCGGG10.819672131147541No Hit
GCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACA10.819672131147541No Hit
ACTGGAGAAAGAAAGAGAAAATCAGTTCGTGGTTGCATTGTGGATGCAAA10.819672131147541No Hit
GGCTAGGCGTCATGCTTCTGGATGAAGGTGATGGGGATGGCCACCATGCC10.819672131147541No Hit
GGCGAAGAGCTAGAAGGCCCCGGGCGAGAAGACCACTAGGCCTCCTCCGC10.819672131147541No Hit
CTCAGATGTTGGGTGTAAGAAATGATGTCTGAGATAAGAAGATCTGACTC10.819672131147541No Hit
CTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAA10.819672131147541No Hit
GGGTGAGAAGATGACAGAGGAAGAAGTAGAGATGCTGGTGGCAGGGCATG10.819672131147541No Hit
ATTGAAATGTGTATTGCACTCACATATTGTAGATGAAGAAACTGTGACTC10.819672131147541No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT10.819672131147541No Hit
GTGCCTGAAATCCCGGGAGATTTTTCTGAGCCAGCCCATTCTTCTGGAGC10.819672131147541No Hit
GTCCTCAGAAACTCCTCATTCTCCTCATATCCCTCAACCGGCCCTTTCGG10.819672131147541No Hit
GCTCACAGGCAAGGTGTAGGGGGGTATTTCCTCGAAAGTCTCGGAGCTCA10.819672131147541No Hit
GTCTAAGTACGCACGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAAT10.819672131147541No Hit
CGCTTGGCCTTTCTCTCCAGCTGCAGTAGCATGATGTCGTTGGAGAAGTT10.819672131147541No Hit
GTGTGGTGGTGGGTGCCTGTAGTCCCAGCTACTTGGGAGGCTGAGGCAAG10.819672131147541No Hit
CTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTAGTTTAAA10.819672131147541No Hit
CTTTTAAATAACATACTCTTGTTCCGGAAGGCAGTTAGCCGTTCATCACT10.819672131147541No Hit
GTTGTTGATGAGATATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAA10.819672131147541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTACAAATAAGGA10.819672131147541No Hit
CCTGGGCCCCGGTCACGTCGCCAACCATCTTCCTGTCCCTAGACTTCACG10.819672131147541No Hit
CCCCAAAACATTCCCATAAAGCATTTTAAAACTCCTTTTTCAGGTGAAGA10.819672131147541No Hit
GCTAATTTTTGTATTTTTAGTAGAGATGGGGTTTGGCCCTGTTGGGGGGG10.819672131147541No Hit
AAATAAATGACTTCTTAAGAAAATATGATTTGCTAAAATTGATCCAAATA10.819672131147541No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAA10.819672131147541No Hit
ATACAAAATGTACCCACTAAGTCTAGGTCATTAGAGCTCTTTCTGTTTCG10.819672131147541No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAATCTGCGTTTATAC10.819672131147541No Hit
CTGGAGTGCAGTGGCACAATCTTGGCTCACTGCAAGCTCTGCCTCCCAGG10.819672131147541No Hit
GTATCAACGCAGAGTACATGGGACTGGCTGCTCTGAAAAGCCATCTTTGC10.819672131147541No Hit
GAATAAAGACCAAAGGGTCCTCTCTACCTTTGAAAACTCTCCTGGGGTGG10.819672131147541No Hit
TCTGTATCCTCTGTGGTTCCAGTTCCAGCTGAACTTGTGACAATCCCAAA10.819672131147541No Hit
TGGTTGTTCAGGTCCTAGGGGTTCCAAGTCCCCAGTCACCTTGAGGACTC10.819672131147541No Hit
GTCTCAGGTGCATCTTCTCAGGCTGTTACGTCAGGGCTCCTGAACTCCCC10.819672131147541No Hit
CCATTTCATTCCATTCCATTCCATTCTATGCCTTTCCACTCGTGTTGATT10.819672131147541No Hit
GGCCACGGCCCGCTTGTCTCAACCACCGCAGCCTTTGCTGCAGCCATCTC10.819672131147541No Hit
CAATCTCACTGCTCTTGAAGTTTGTGAGCACTTTCAGCACCACGCCCTGG10.819672131147541No Hit
ATACATCACCTTACGGTTTAGCAGAGACCTGTGTTTTTGATAAACAGTCG10.819672131147541No Hit
CTCCAGATGTCTAACAAAATGACTCTTAATAGAAAGTAGCTGTCACATTG10.819672131147541No Hit
ACGCAGAGTACATGGGGAAAGTCGGGATCGTCGGTAAATACGGGACCCGC10.819672131147541No Hit
GTGCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATG10.819672131147541No Hit
GAGCAAAGCGCGAGTCCGCTTCAACTTGATGGAAAAGATGCTTCAGCCTT10.819672131147541No Hit
GAGGGCTGCTGGGTGGCCTACACGCTGGAGCAGCAGGACGGGATGGACCG10.819672131147541No Hit
ATCTTTAGCTTCCTCAAACAAAACAATTATCAGCCAAGAATTTTGTATCC10.819672131147541No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAAGTACTTT10.819672131147541Clontech Universal Primer Mix Long (95% over 24bp)
CTACAGTGCCTCGCCGCCTGGGCCCCAAAATAGCTAGCAGAATCCGCAAA10.819672131147541No Hit
TGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCAGATGATATCC10.819672131147541No Hit
CGGCCGGGGCCGGAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATG10.819672131147541No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGT10.819672131147541No Hit
GAGGTGTGGGTGTTCGTTTCTCAGGTAAAACATGGCTAAAAGCTTACGGA10.819672131147541No Hit
ATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACACTTTCTCGG10.819672131147541No Hit
GCCCACAGGTAGAGTCACCAAAAACATAGCCAACACATGCCTGGTCCACT10.819672131147541No Hit
GCTTCAAATTGCGCTTGTCGGCTATACAAACGCAGGGAAATCAACATGGT10.819672131147541No Hit
GGAGGAAATAGGACCAAGACCCCTGGACCTGGGGCCCAGTCGGCCCTCAG10.819672131147541No Hit
GATTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGAC10.819672131147541No Hit
CACATCCACCGATATCCCGCAGCGCGACGATCAGTTGCGCACATCCCCGG10.819672131147541No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG10.819672131147541No Hit
CGCAGGCGACGGGAAGTCCCACTTAATTCATTTGAGAGAATCTGGTGTGT10.819672131147541No Hit
GCCCCACACCACGCACAGGCCGCCCAGCAGGAAGAAGCCATATGCTGCTC10.819672131147541No Hit
TGACTGCAGGGCTTGAGAATGGCGGAGGGCTTAGGCAGTTTGGACAGGGT10.819672131147541No Hit
ATTTAGGGCTGGCGGATCCATGCTGTGGGAGGTGGAGGATGTGAAGAAGC10.819672131147541No Hit
GTTGAACGAAAGCTGGTCGCGGCGCGAATAGCGCAGCACATGCGCCAGCC10.819672131147541No Hit
GGGTTAAACCTGTGGAGGCAGGACCTGGGAAGGGGCACGGGGACAGATGA10.819672131147541No Hit
AGCCTGGCCAATATGGGAAAACCCGTCTCTACTAAAAATACAAAATTTAG10.819672131147541No Hit
CATCTGCGCCGCGATGCGGTCGGCCGTGGCCTGTGCGCGTTCGGGCGAAT10.819672131147541No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)