FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003644840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1085
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT70.6451612903225806No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG50.4608294930875576No Hit
CTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTG50.4608294930875576No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA50.4608294930875576No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC50.4608294930875576No Hit
CTTATGTATTTGGGGTTTACCGTGGGCTCAATGCCGGCAATCCACAGATA50.4608294930875576No Hit
CTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATA50.4608294930875576No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG40.3686635944700461No Hit
GAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATA40.3686635944700461No Hit
GTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGG40.3686635944700461No Hit
AGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACC40.3686635944700461No Hit
ACGCAGAGTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCT40.3686635944700461No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA40.3686635944700461No Hit
GTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT40.3686635944700461No Hit
TCTATGATAATGTTGCATTTGGCCCAAGAATTCATGGAATTAAGGATAAA40.3686635944700461No Hit
ATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCT40.3686635944700461No Hit
ATGCGGGACATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAGCA40.3686635944700461No Hit
GTATATGATGTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTC40.3686635944700461No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC40.3686635944700461No Hit
CCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTC40.3686635944700461No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA40.3686635944700461No Hit
GGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCA30.2764976958525346No Hit
TGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACAT30.2764976958525346No Hit
CCTCCGCCTTGCCGGAAAGGTTGATCCCTTGTCCCGTCAGACGCGGCAGT30.2764976958525346No Hit
TTCTTAGCTGTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGT30.2764976958525346No Hit
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT30.2764976958525346No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT30.2764976958525346No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG30.2764976958525346No Hit
CTGTACCACAACGCTCCCGGTTCTTCGACGCTCAGTTCGGTACCCAAGAT30.2764976958525346No Hit
CTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTG30.2764976958525346No Hit
GTTACACTATAGTCATCGTCCATAACACGATCTAGTGAAACCTTCAAGCA30.2764976958525346No Hit
CTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCA30.2764976958525346No Hit
GTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAA30.2764976958525346No Hit
GTCGTCTACCAGCTCGATAATTGGCGCAAGTTGTAGGTCGGGGGCACCAC30.2764976958525346No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC30.2764976958525346No Hit
TCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGC30.2764976958525346No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCC30.2764976958525346No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA30.2764976958525346No Hit
GGCCAGGAAACATTTAATATCCCTAACAAGCACGGTCCACCAACGGCATT30.2764976958525346No Hit
ACGCAGAGTACATGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAA30.2764976958525346No Hit
CGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGC30.2764976958525346No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT30.2764976958525346No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA30.2764976958525346No Hit
GCTAAGACAGCTAAGAAAGCTCCAATTGGTAGAGTTCCAACATCTCCTGG30.2764976958525346No Hit
AGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGC30.2764976958525346No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG30.2764976958525346No Hit
TGTTGGAACTCTACCAATTGGAGCTTTCTTAGCTGTCTTAGCAGTAGTTT30.2764976958525346No Hit
GTCATCACATGGATCTCATAGTCACCACATAGATCGCGACATATCAGCAT30.2764976958525346No Hit
GTACATGGGATCGATGACGCGAATTGGGGACATTCAAATGAGCATCCTAG30.2764976958525346No Hit
AAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGA30.2764976958525346No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT30.2764976958525346No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC30.2764976958525346No Hit
GTTCTTTATGGGGTATCGCTCTTAAGCAGCGCACTTCTGCATAACTATGC30.2764976958525346No Hit
GTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGC30.2764976958525346No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT30.2764976958525346No Hit
CATCTGCTTCAAGTTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCC20.18433179723502305No Hit
TAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCCCCG20.18433179723502305No Hit
GAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTT20.18433179723502305No Hit
AGTAAAAGAAGCGGATTTAATTCTGCATTTAATTGATTCTTCAAATGAGG20.18433179723502305No Hit
ACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCT20.18433179723502305No Hit
TTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGAG20.18433179723502305No Hit
ATTAACCATTTCCTTAAATTCTTCTGGGTCTGCTGACAAAGCATGATCAG20.18433179723502305No Hit
ATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTG20.18433179723502305No Hit
TAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCCT20.18433179723502305No Hit
CTTCTGGGTCTGCTGACAAAGCATGATCAGGACCTTCCATATTTTTATCT20.18433179723502305No Hit
CCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGGA20.18433179723502305No Hit
CGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGT20.18433179723502305No Hit
GTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTC20.18433179723502305No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT20.18433179723502305No Hit
CTAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGG20.18433179723502305No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC20.18433179723502305No Hit
GCTGGTAGACGACCAGCGCTGACGAGCTGGCGCAATGACGACCTAATTGG20.18433179723502305No Hit
GGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCAC20.18433179723502305No Hit
GCAAAAATATCTAATATCAATTGCGTGCGGTCAATCATCTTCACTTCAAT20.18433179723502305No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG20.18433179723502305No Hit
CCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGC20.18433179723502305No Hit
TCAACGCAGAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAG20.18433179723502305No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA20.18433179723502305No Hit
CCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGAC20.18433179723502305No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT20.18433179723502305No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG20.18433179723502305No Hit
GTACATGGGAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGC20.18433179723502305No Hit
ATCACCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAAT20.18433179723502305No Hit
TATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACT20.18433179723502305No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG20.18433179723502305No Hit
GCTGATAGCATCCGAGCTAATGTATCAGCCTATACGCTAATGTCAGAGTC20.18433179723502305No Hit
GTTTGCCTTCTCTCGTTCTCGCCCGCTTTGCAAAAATATCTAATATCAAT20.18433179723502305No Hit
ATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAAT20.18433179723502305No Hit
GGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCTCTTTACAG20.18433179723502305No Hit
AAGCAGCCTCAATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCG20.18433179723502305No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA20.18433179723502305No Hit
TTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACG20.18433179723502305No Hit
GTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCA20.18433179723502305No Hit
CTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTG20.18433179723502305No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA20.18433179723502305No Hit
GCGCTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCA20.18433179723502305No Hit
ATTTCCGGCATCAAGCGTTTCTCCCTACTAATATCCTTCCTTATGTCCCT20.18433179723502305No Hit
CCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGGAAGATA20.18433179723502305No Hit
CTTCGTGCCGACACGAAGCAGCCTCAATATCTAACACGAGGATGTTCCTG20.18433179723502305No Hit
GGCTTACGGTAGGCCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA20.18433179723502305No Hit
GGTATGGTCTTGGGCATACCCGATACATGCAACACAGCTCGGGATGTTCA20.18433179723502305No Hit
GCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA20.18433179723502305No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT20.18433179723502305No Hit
GACTATAGTGTAACAGGTGCAGAGCTTAACTTTGGACACATGACACTCAG20.18433179723502305No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC20.18433179723502305No Hit
TTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAG20.18433179723502305No Hit
TTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCGCGGTTTTGACTGCG20.18433179723502305No Hit
GTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGG20.18433179723502305No Hit
CCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTT20.18433179723502305No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT20.18433179723502305No Hit
CATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCGTATCTTCCGGGA20.18433179723502305No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG20.18433179723502305No Hit
ATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA20.18433179723502305No Hit
TTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTAT20.18433179723502305No Hit
AATCTATATTACTCACAATATCCTAAAAACGATCAGCCGGCAGGTTCATT20.18433179723502305No Hit
AGACAGCCAGTTTAGGCGTAGCACTCGCTGTTATAGCCGAGCACACTCCG20.18433179723502305No Hit
TTTATAATTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATATCT20.18433179723502305No Hit
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCC20.18433179723502305No Hit
GTCAAAACCGCGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTG20.18433179723502305No Hit
TATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTT20.18433179723502305No Hit
CCACGCTCGCTTGGCAGTAACGCGAGCAGGGGGCGTCCCTATACGCGATG20.18433179723502305No Hit
GCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAAC20.18433179723502305No Hit
GTATGCCATGTATGCTGATATGTCGCGATCTATGTGGTGACTATGAGATC20.18433179723502305No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA20.18433179723502305No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA20.18433179723502305No Hit
GTCCCATCTAAACCGCCGTGCCAATAGCTTTAAAGGCCAGGAAACATTTA20.18433179723502305No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20.18433179723502305No Hit
TATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTC20.18433179723502305No Hit
ATATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTT20.18433179723502305No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG20.18433179723502305No Hit
CGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC20.18433179723502305No Hit
AAACAGCAGACGGAAAAGTACTGACCCTGTCTCTTATACACATCTGACGC20.18433179723502305No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT20.18433179723502305No Hit
GTCTGATATTGGGGAAATTTGGGAAGCAGTTAAAGTTTTAGAAAATAATG20.18433179723502305No Hit
GCTTCCATCAGTCCAAACATCAAGTGTAAGTTTATCATAGTTTGCAACTT20.18433179723502305No Hit
GTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGGTTTTTACTAT20.18433179723502305No Hit
CATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAAT20.18433179723502305No Hit
GTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATT20.18433179723502305No Hit
ATCTAACACGAGGATGTTCCTGTTGATACCGTCTGCGATAGGCTAGTTCA20.18433179723502305No Hit
GACTCAACGCCCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGA20.18433179723502305No Hit
GATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCG20.18433179723502305No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC20.18433179723502305No Hit
ATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAG20.18433179723502305No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG20.18433179723502305No Hit
AAAAATGGCACTCTGATTAAACTGCATTACAGCCTGCAGGACACCTTGGG20.18433179723502305No Hit
CTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTAC20.18433179723502305No Hit
GCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTT20.18433179723502305No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT20.18433179723502305No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT20.18433179723502305No Hit
AAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAA20.18433179723502305No Hit
GAGCTCGAATTCCCCCATGTACTCTGCGTATCAACGCAGAGTACATGGGG20.18433179723502305No Hit
ATGCCCGGGCTCAGAATCCAAGGAGCAAAACTGCGTCGGTTTAGCTGAGA20.18433179723502305No Hit
CCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATCCCTTGTCCCGTCAGA20.18433179723502305No Hit
ATTTTTATCTAAGGTAAAGTGCTTCTCAATAACATCCGCTCCTAAGGCAA20.18433179723502305No Hit
GGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTT20.18433179723502305No Hit
CGGAAAAGTACTGACCAGCGTCACACAAAAACGGAACAGGACTGACGCCG20.18433179723502305No Hit
AAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGA20.18433179723502305No Hit
CAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCG20.18433179723502305No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA20.18433179723502305No Hit
GGATCGAGGTCATGACTGGACACTGCATCGGAAGACACTTCGTGCCGACA20.18433179723502305No Hit
AGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCG20.18433179723502305No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.18433179723502305No Hit
GTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGA20.18433179723502305No Hit
CACACACACACACACACACACACACACACACACACACACACACACACACA20.18433179723502305No Hit
GTACATGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCAC20.18433179723502305No Hit
ATTAAGCCGTAGTTGAATTTATGGAGTGGTGCCCCGGGAAAAGGTATAAA20.18433179723502305No Hit
GTATATTCAATATCCCTGTGGGATATTCTGACCATACATTGGGAATACTC20.18433179723502305No Hit
GATAAGAACCCTCCAGCTCGGGGCCTCAAACCGATATTCCATGTGGGCCA20.18433179723502305No Hit
CGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTAC20.18433179723502305No Hit
ACGGAGTGTGCTCGGCTATAACAGCGAGTGCTACGCCTAAACTGGCTGTC20.18433179723502305No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)