FastQCFastQC Report
Tue 10 May 2022
EGAF00003644937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13170
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT1321.0022779043280183No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA820.6226271829916477Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC730.5542900531511009No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT570.43280182232346237No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT560.42520880789673504Clontech Universal Primer Mix Long (96% over 26bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA560.42520880789673504No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA510.38724373576309795No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA490.3720577069096431No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG480.36446469248291574No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG470.3568716780561883No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG420.31890660592255127No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG410.3113135914958238No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG380.2885345482156416Clontech Universal Primer Mix Long (96% over 26bp)
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT370.2809415337889142No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT360.2733485193621868No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC320.24297646165527714No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT300.22779043280182232No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG300.22779043280182232No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC290.2201974183750949No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT280.21260440394836752No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT260.19741837509491267Clontech Universal Primer Mix Long (96% over 26bp)
CAGTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTAT260.19741837509491267No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG250.18982536066818526No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG250.18982536066818526No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT250.18982536066818526No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA250.18982536066818526No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA240.18223234624145787No Hit
GTCCTTACAAGTCCGCTCCTCGGGGAGCTTGATTGATAATTCTGTATAAG240.18223234624145787No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC240.18223234624145787No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG230.17463933181473046No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA230.17463933181473046No Hit
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT230.17463933181473046No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT220.16704631738800305No Hit
GTAAACTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTA220.16704631738800305No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG220.16704631738800305No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG210.15945330296127563No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC200.15186028853454822No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT200.15186028853454822No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT200.15186028853454822No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG190.1442672741078208No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC190.1442672741078208No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG190.1442672741078208No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG190.1442672741078208No Hit
TCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGC190.1442672741078208No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT190.1442672741078208No Hit
CCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGACA190.1442672741078208No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC180.1366742596810934Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC180.1366742596810934Clontech Universal Primer Mix Long (96% over 26bp)
GCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCT180.1366742596810934No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA170.12908124525436598No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG170.12908124525436598No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT170.12908124525436598No Hit
TCCCTATACGCGATGGATCGAGGTCATGACTGGACACTGCATCGGAAGAC170.12908124525436598No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG170.12908124525436598No Hit
TACTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTAT160.12148823082763857No Hit
ATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACATTCAAA160.12148823082763857No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC160.12148823082763857No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT160.12148823082763857No Hit
TCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGC160.12148823082763857No Hit
CTAGTAGCATCCCGATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGC160.12148823082763857No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC160.12148823082763857No Hit
TATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCA160.12148823082763857No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT160.12148823082763857No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC160.12148823082763857Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG160.12148823082763857Clontech Universal Primer Mix Long (96% over 26bp)
CTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGG150.11389521640091116No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG150.11389521640091116No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC150.11389521640091116Clontech Universal Primer Mix Long (96% over 26bp)
ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC150.11389521640091116No Hit
GGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCG150.11389521640091116No Hit
GCATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTA150.11389521640091116No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA150.11389521640091116No Hit
CTTTAGGTGGGCCTTGGCTTCCTTCCGCAGTCAAAACCGCGCAATTATCC150.11389521640091116No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC150.11389521640091116No Hit
TGATAATTCTGTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAG150.11389521640091116No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT150.11389521640091116No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT150.11389521640091116No Hit
ATCTTTTCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTG150.11389521640091116No Hit
GTACATGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAA150.11389521640091116No Hit
GCTATAACCCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTC140.10630220197418376No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA140.10630220197418376No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC140.10630220197418376No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC140.10630220197418376No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG140.10630220197418376No Hit
GTACATGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCA140.10630220197418376No Hit
ATCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGA140.10630220197418376No Hit
AACATGCACAGCGGTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTG140.10630220197418376No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG140.10630220197418376No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC140.10630220197418376Clontech Universal Primer Mix Long (96% over 26bp)
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA140.10630220197418376No Hit
TCCTAGTCACCGCGTTTAAAATGAACCTGCCGGCTGATCGTTTTTAGGAT140.10630220197418376No Hit
TCCCTATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGG140.10630220197418376No Hit
GTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATCTTTTCCCTAT140.10630220197418376No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC140.10630220197418376No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG140.10630220197418376No Hit
AACCAGATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGAC140.10630220197418376No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG140.10630220197418376Clontech Universal Primer Mix Long (96% over 26bp)
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA140.10630220197418376No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)