FastQCFastQC Report
Tue 10 May 2022
EGAF00003644941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169355
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA11280.6660565085176109Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT9550.5639042248531192Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3930.2320569218505506No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3830.2261521655693661Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3720.21965693366006317No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC3590.21198075049452333Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC3120.18422839597295623No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT3050.18009506657612706No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT2910.17182840778246877No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT2700.15942841959198134Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC2680.15824746833574443Clontech Universal Primer Mix Long (96% over 26bp)
ATATAAATGCGTAGGGGTTTTAGTTAAATGTCCTTTGAAGTATACTTGAG2650.1564760414513891No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG2630.1552950901951522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG2600.15352366331079687No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT2430.14348557763278322No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA2420.14289510200466476No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG2420.14289510200466476No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC2320.13699034572348026No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA2280.13462844321100648No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC2210.1304951138141773No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG2150.12695226004546661Clontech Universal Primer Mix Long (96% over 26bp)
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG2050.12104750376428212No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA2010.11868560125180834No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG1950.11514274748309763No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA1910.11278084497062382No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT1880.11100941808626849No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT1820.1074665643175578No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT1810.10687608868943935No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1780.105104661805084Clontech Universal Primer Mix Long (96% over 26bp)
GTATATAGGCTGAGCAAGGGGTGGTGAGGTTGATCGGGGTTTATCGATTA1770.10451418617696555No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC1750.10333323492072864No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA1740.10274275929261019No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC1740.10274275929261019No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC1730.10215228366449174No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG1720.10156180803637331No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC1710.10097133240825486No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC1710.10097133240825486No Hit
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC1700.1003808567801364No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)