FastQCFastQC Report
Tue 10 May 2022
EGAF00003644945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003644945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353809
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8600.2430690005059227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8180.23119818885330787Clontech Universal Primer Mix Long (96% over 26bp)
GATTAAACTGCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTA4480.12662199096122484No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC3790.10711994324621475No Hit
GCATTACAGCCTGCAGGACACCTTGGGCCAGCTTGGTTTTACTCTAGATT3730.10542411301012694No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3570.10090189904722605No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)