FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTGGTCTGCGGCCTGTCAAACAGGTTCGGGTTCAGTTCTGTCCCTTC25.405405405405405No Hit
GATCAGTCTAGGTGCAGCTGCCAGATCCTTCAGCGTCTGCATCTCGGCGT12.7027027027027026No Hit
GCCCTGGTCCAATGGCCCCATACCCACCTTATCCACCACAACCAATGGGT12.7027027027027026No Hit
GGGGAGCCGAGGACCAGGTCGCCCTCCGTGTCACTGCTGTCTCTTATACA12.7027027027027026No Hit
GGACTAGACTCTTCTGGCAGGCAGAGAGCTCCTCCTCGGCCAGTGTTCGA12.7027027027027026No Hit
CTGGAGTACAGTGGAGCAATCTCGGCTCACTGCAACCTCCGCCTCCTAGG12.7027027027027026No Hit
TATCAGGGATGCGGGCAGCACCCCTCCTCTGTGATGTGATGTTGGGGACC12.7027027027027026No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12.7027027027027026No Hit
GGCGAGGCGCGACCGGAGGACGCCGGCGCCCCGTTCGAGCAGGCCGGTCA12.7027027027027026No Hit
AAGGAGATGACCCCGATGCCCGATGCCAGGAGGCCATTGCGGAACCAGGA12.7027027027027026No Hit
CTCCAGGATGATGTTCTTCTTGTCCTCACTCAGGCAGAAGAGCACCGCCT12.7027027027027026No Hit
GCTCTCGCAGGTCGGGCTGGATCTCATCCATGGCGCGGATCTCCAGGCGC12.7027027027027026No Hit
GCAGGCACACAGGGCACGGCGCTCTGCCTGCAGCAGTAGGGTCTCCGTCC12.7027027027027026No Hit
ACCTCCACATGCTCCGCCTCCAGGTCCCGCTGCAGCCTGTCTCTTATACA12.7027027027027026No Hit
GTACATGGGGCGTCGAGGGGAGAGGCAGCAGCCGCGATGGACGTGTTCCT12.7027027027027026No Hit
CGCCACAATGGAGTAGGCACCAAAGTACCACTGGGTGAAGCGCCCAGCTG12.7027027027027026No Hit
TTGCCAATGGTGATGACCTGGCCGTCAGGCAGCTCGTAGCTCTTCTCCAG12.7027027027027026No Hit
GGCCAGAGGCACGTGCGGCGGCGGCGGGAGGCAGAGGTCCTGGCGGAGGT12.7027027027027026No Hit
GCGCGCACCTCAGCCCTGAGCCCGGCGGCGGCAGGCGTCGCTGTCTTTTC12.7027027027027026No Hit
ATATCATCCAGGCCAGGCACGATGTCTCAACGCCTGTAATCCCAGTGCTT12.7027027027027026No Hit
GTGTTCACTCAGGGAGCATTCCCACGCTGTGTTCATTCACCGAGCACGCG12.7027027027027026No Hit
CTCCTGACCTCAAGTGATCTGTCCTGGCCTCCCAAAGTGTTGGGACTGTC12.7027027027027026No Hit
ATACAGCTCAGTGAAGGTGGCGAGGTCCAGATCGCTGGTGCGGATGATGC12.7027027027027026No Hit
GCCTTCCTCTCCTGGTTCCGCAATGGCCTCCTGGCATCGGGCATCGGGGT12.7027027027027026No Hit
ACCCTGCGCCGCACCAAGGAGGAGATCAATGAGCTGAACCGCATGATCCA12.7027027027027026No Hit
GCCCGGGATGTTGGACTTCACGGGCAAGGCCAAGTGGGATGCCTGGAATG12.7027027027027026No Hit
GAGGTGTACTTCGCCGAGAGGGTGACCTCTCTGGGCAAGGACTGGCATCG12.7027027027027026No Hit
ATCTCTCACTTCTTCCACTCGTTCTTTTCGTAGTCCCACTTGGAGGCTAA12.7027027027027026No Hit
ATCCTCACGAAGGCCTCCAGCCGGTCGATCCTGCTGTAGATGCGCCGCAT12.7027027027027026No Hit
TCTAGGTGGAGCGAGGGGAGTGGCCAAAAACCATGCCAACATGAATGGTT12.7027027027027026No Hit
CAGTGGCACGATCTCGGCTCACTGCAACCTCCACCTCCTGAGTTCAAGCT12.7027027027027026No Hit
CTCGATACGGTGCACTCTGCCTGTCTTCCACTTCCTTTCCATCTGCATAC12.7027027027027026No Hit
CACCTACGGCCTCTACTCCTTCCACCGGGGCAACAGCCAGCGCTCTCAGC12.7027027027027026No Hit
CCTGGACCTGCGGAGATGCTGCCTAGCTGCAGCGCGAACCCTGGGGAGAA12.7027027027027026No Hit
GTATCAACGCAGAGTACATGGGGCTCTTTCCCTCGGAGCGGGCGGCGGCG12.7027027027027026No Hit
GTCCAGGAGCGCAGGTCCTCGTTCAGGGCGATGTAATCCTTGCCGTCGTA12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)