FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37
Sequences flagged as poor quality0
Sequence length140
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGTCTGCAGGAAGGTCCTCGTCGATTCCACGTTTTTCTCGAAGGGACAG25.405405405405405No Hit
CTTGAACTCAGGAGGTGGAGGTTGCAGTGAGCCGAGATCGTGCCACTGCT12.7027027027027026No Hit
CAAACAAGTACACCGTCCTGTGGCCGACGCCTCCGCCATCCTCCTCAGTC12.7027027027027026No Hit
CTCCTTGGCGTCCGTGAAGATGGTGGTCTTGTGGCGCCGGATCATGAGGA12.7027027027027026No Hit
GCCTAAGGCAGGTGGTGAACGTGTATGCAGATGGAAAGGAAGTGGAAGAC12.7027027027027026No Hit
GTGCTGGACCAGATCTTTCCCCACAGCAAGACTGGGCCTGAGGGTCCCTC12.7027027027027026No Hit
GTCTCTGATGGTGTCATCAAGGTGTTCAACGACATGAAGGTGCGTAAGTC12.7027027027027026No Hit
ACTGAGGGCCGCCTCACCCTGCTGCTCAGACTGGGCCACCGCGGCCTCCA12.7027027027027026No Hit
AGCTTTGACAAAGAGTGGGTGGCCAAGCAGACCAAGAGGATGCTGGACAT12.7027027027027026No Hit
CAGTGACACGGAGGGCGACCTGGTCCTCGGCTCCCCCTGTCTCTTATACA12.7027027027027026No Hit
GCCCACGGCCGCGCCCCCCAGCGTCAGCTGCATGGGTCCTCGCAGCGCCG12.7027027027027026No Hit
CTCCTGAGGTCCACCCTGCCAGCCGTACTGTGGGTATCCTTGGTAGGGGT12.7027027027027026No Hit
GTGCCCACGTGGCGCGTGCTCGGTGAATGAACACAGCGTGGGAGAATCGT12.7027027027027026No Hit
CCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGA12.7027027027027026No Hit
CTGGAGAAGAGCTACGAGCTGCCTGACGGCCAGGTCATCACCATTGGCAA12.7027027027027026No Hit
GGCCATGTGGGCCAACGAGCAGGCGCTGGCGTCCGGCCTGATCCTCATCA12.7027027027027026No Hit
GCGTCTTCCCACATTTCTCGCACTTCAGGCAGGGCCGATGCCAGTCCTTG12.7027027027027026No Hit
CCCTGTGGCTGGTGCTGCCTGGAGTCCTTGTGCTTGATGCTGTGAAGCAC12.7027027027027026No Hit
CGCCTGTAGTCCCAGCTATTCGGGAGGCTGAGGCAGGAGAATTGCTTGAG12.7027027027027026No Hit
CCTCTGAGTCAGGCTGTGAGTCTAGGTCCCCAACATCACATCACAGAGGA12.7027027027027026No Hit
GCGGAGAGCCGGGCGACGGTACGCGGGGCGACGCCGAGATGCAGACGCTG12.7027027027027026No Hit
CCCGCCGGGAGTGCCGTCCCCGCGCAGAGCCGCCCATGCGTGGACTGCCA12.7027027027027026No Hit
CCTATCATCACTTCCGTCCAGGTTTCCAAACTCCGTGGTTCCTTCAACCA12.7027027027027026No Hit
ATCAACGCAGAGTACATGGGGGAACCTGCGCGGCTACTACAACCAGAGCG12.7027027027027026No Hit
AGCCTAGAGGACCTGCTTACCAGAGTGGACGAGTTCGTGGGCATGCTGGA12.7027027027027026No Hit
GGCTGCGACCGTGAAACCCTGGGCGGCGATCCGGGTGCGCATCATGAGCT12.7027027027027026No Hit
GAGCTGGATCGTGCGGACGCCTGGCTCCTCCGAAAAGCGCACGAGACAGC12.7027027027027026No Hit
TCCCAACACTTTGGGAGGCCAGGACAGATCACTTGAGGTCAGGAGCTGTC12.7027027027027026No Hit
CCACAAAGCACTGGGATTACAGGCGTTGAGACATCGTGCCTGGCCTGGAT12.7027027027027026No Hit
GCCATGGAGCGGATCAAGGAGGACCGGCCCATCACCATCAAGGACGACAA12.7027027027027026No Hit
CGTCTGCTCACCGTCATCACCGCTGAGATCAAGTGTGTAAGCATCATCCG12.7027027027027026No Hit
CAGCTGGGACAGCTCCACCTTCAGGACGTCCAGCTGTTTCAGCAGCTCCT12.7027027027027026No Hit
CGTCGTGCACGGCTTCCCGTCCTCCGTGGCCGCCCTTCGGTTTGAGTGGG12.7027027027027026No Hit
GCTGCAGCGGGACCTGGAGGCGGAGCATGTGGAGGTCTGTCTCTTATACA12.7027027027027026No Hit
GTAAGCTTTCATGGCATCTTCCTTGGAAGTCCCTTTCAGCTCATTCCAGG12.7027027027027026No Hit
CTCTGTGGCTGCGCCCTGATGTTGCTGATTTTCCGGATGAGCTCGTCGTT12.7027027027027026No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)