FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length140
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1811.688311688311687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC106.493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG85.194805194805195Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT63.896103896103896Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC42.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT31.948051948051948Clontech Universal Primer Mix Long (100% over 23bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31.948051948051948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA31.948051948051948Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GGCGGAGGTGGCCCATGAGCACCGCGTCGGCTCGGCCGAGCTGGCGCTCG21.2987012987012987No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TCTCTTTTCGGTCTGAAAAAATAATCCGTTTAATTGAAAAACCTGGAGGA21.2987012987012987No Hit
CGGTGTCCCAAAGCAGGCGCCGGGCCACGGGGTCCATGCCAGTGGACGGC21.2987012987012987No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21.2987012987012987Clontech Universal Primer Mix Long (96% over 25bp)
TCCTAAGACCTGGCACGTGATAATTTTAATGAAATATCTGGGTGAATGGA21.2987012987012987No Hit
GCGTTGATACCACTGCTTGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
CATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGAAACAAA10.6493506493506493No Hit
GTGGTATCAACGCAGAGTACCTGGGAAGCAGTGGTATCAACGCAGAGTAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGAGTTGATAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG10.6493506493506493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GAAGGTGACTGGGGAGAAATTGAGCGTGATTTCGACTTCGGATCAGATGC10.6493506493506493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCAGCGT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TTCTGGTACCCCAGAACCTGAAACATCGTCTTGAAGACATGGAACAGGCT10.6493506493506493No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG10.6493506493506493No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAACCAGCCCCTGGCAC10.6493506493506493No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAGAAACGT10.6493506493506493No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGGGATAGCACAGCCTGGATAGCAACGTACATGGCTGGGGTGTTGA10.6493506493506493No Hit
GTACATGGGAAGGGCGAAGAAAAGAGGCCTTCCTCAGTTGGGGACCCTGG10.6493506493506493No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGAACATG10.6493506493506493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACAGGGGTAAGCAGTGGTATCAACGCAGAGTACAGGGGTAAGCAGTGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 27bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493Clontech Universal Primer Mix Long (95% over 22bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACACTGCGTTG10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACGTGGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAAGCAGTGGTATCAACGCAGAGTACAGGGTAAGCAGTGGTATCAAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 27bp)
ACATGGGAAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA10.6493506493506493Clontech Universal Primer Mix Long (95% over 23bp)
CACTAACGACATGGACATCCACACGAAAACCGCGATGGACGTCTTTCACG10.6493506493506493No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTC10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC10.6493506493506493No Hit
CTATAAGGTTTCCATTCACTGTGAAAGACATACTTTCTTCTATTTGTCCT10.6493506493506493No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCAGTAATATGCGGTGATA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGAAG10.6493506493506493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGAAGCAGTGGTATC10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TGTAAAAAACAAATTATCTTAAATAGCCAAGACCATTTGAATAATATGTG10.6493506493506493No Hit
GCTTCCATTACTCTGCGTTGATACCACTGCTTCCATTACTCTGCGTTGAT10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATTACTCTGCGTTGATACCACTGCTTCCAATACTCTGCGATGAT10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGTG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTCAAAAGCTGCCGGCTCTCATGGACGGTCTGACCGCCGTAGGCCGGATC10.6493506493506493No Hit
AACCAGGTGCTCACTCAGGGCCAGGCGTTGCTGGGCTTCCGGCGCCCCAA10.6493506493506493No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG10.6493506493506493No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTATGGTGCAGAGTCCCAGCAAGCAGAGGTCTTTGTATCTGTTGAAT10.6493506493506493No Hit
GTCGAGGAGCGAGGTGCGGGTGTCGAGCTCCAGTTCCCGGCGCTCGCCGT10.6493506493506493No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC10.6493506493506493No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGAACATGGGAAGCAGTGGTA10.6493506493506493Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCAGCGTAGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.6493506493506493Clontech SMART CDS Primer II A (95% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GGAACGCTTTCTGGAGATCACCGGGACGCCTGCGGCATCAAGAGCCGTGG10.6493506493506493No Hit
CGGTCGCGTTCGACATCTCGCTGCCCGAGCAGCGCGCGCTGTACCGTCGC10.6493506493506493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)