FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA149.090909090909092Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT138.441558441558442Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA53.2467532467532463Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC42.5974025974025974Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG42.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC42.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG42.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA42.5974025974025974Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG31.948051948051948Clontech SMART CDS Primer II A (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG31.948051948051948Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTAT21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.2987012987012987Clontech Universal Primer Mix Long (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGGCCTCTAGGGTGACCTAGAGCCGCCTTCAGATGTGACCCGAGTA21.2987012987012987No Hit
ACGCAGAGTACATGGAAGCAGTGGTACTCTGCGTTGATACCACTGCTTCC21.2987012987012987Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGTGCTCCACACACCTCTGATTATCAAGGAGCTCTCCAAGGTG21.2987012987012987No Hit
TTACTGTGGGCTGTGGGATTAATTTCAACGAGAAGGCGATCCATAAGGAG21.2987012987012987No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21.2987012987012987Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC21.2987012987012987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTGGCTGAGCAGGGCCTCCAGATTCTCGGCCAGGGCCGGCGGCGCGGT21.2987012987012987No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21.2987012987012987Clontech Universal Primer Mix Long (96% over 25bp)
GGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCCGGAGGGGACC10.6493506493506493No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCAACGCAGAGT10.6493506493506493Clontech Universal Primer Mix Long (95% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAACCCTGTGT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
AAAGTTTACTCCGAAATCAGTACCTTTCTGAGATGCATCTGATCCGAAGT10.6493506493506493No Hit
ATCTCGACCGCGAGCCTCAAGGCCGTCGTGTCGTGCGTCAACCGCGCGAT10.6493506493506493No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAACAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTC10.6493506493506493No Hit
GCAGTGGTATCAACGCAGAGTACATGGGCTAAGCAGTGGTATCAACGCAG10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTAC10.6493506493506493No Hit
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAATTAC10.6493506493506493No Hit
ATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATAA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
TGTTAAAGTTACGGGAGGTCAGCTCCGGAATGTAACGGCGACGATGCATC10.6493506493506493No Hit
AGTCAGCCATCTTTGCTGATATCATTCTCAACACAAATTATTTGAAAAAC10.6493506493506493No Hit
GGTATCAACGCAGAGTACCACTGCTTCCCATGTACTCTGCGTTGATACCA10.6493506493506493No Hit
GGTATCAACGCAGAGTACCACTGCTTCCCATGTACTCTGCGTTGATAGCA10.6493506493506493No Hit
GCTTACCCTGTACTCTGCGTTGATACCACTGCTTACCCTGTACTCTGCGT10.6493506493506493Clontech Universal Primer Mix Long (96% over 28bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAGAAACTTGT10.6493506493506493No Hit
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACTCTGCGT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG10.6493506493506493No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAAGGGTTTATAAA10.6493506493506493No Hit
GGTCAGAAGGATTCCTATGTGGGCGACGAGGCCCAGAGCAAGAGAGGCAT10.6493506493506493No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATCACTCTCCTTCCCAAGTC10.6493506493506493No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCTGTCTC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GTTGATGATGCCCCCGGCGTGAACCGTTCGGGGACGAGATGGACAATCTC10.6493506493506493No Hit
GCGACATGCCGTTTGAATACCTCAGAGAAATCGCGCAGGCGCAGCTTCCG10.6493506493506493No Hit
CAGCATCACAACCGGCGCCGGCGGTCGGTTCTCGGCCGCTTCCCACGCGC10.6493506493506493No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT10.6493506493506493No Hit
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT10.6493506493506493Clontech Universal Primer Mix Long (96% over 27bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGAT10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTCCCATGTACTCTGCGTT10.6493506493506493Clontech SMART CDS Primer II A (96% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTATCA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6493506493506493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
ATATAATTGATTGTTCTTCAGTTTTTGAACTGTATCAAATTTTGCAAAAA10.6493506493506493No Hit
ACCCCAGTCTCTGTCAGTTCCAGCACTGTCTGGTGCTGCATCGCAGAAAC10.6493506493506493No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT10.6493506493506493No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAAAAAA10.6493506493506493No Hit
GAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGG10.6493506493506493Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA10.6493506493506493Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGCGTTGAT10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 28bp)
CCCCTGGGCGCCACCTCTGCCCACTCCCATCCTCTCTCCAAGCTCCCATT10.6493506493506493No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC10.6493506493506493No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTATACCAAAGCTTCCCATGTAA10.6493506493506493No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTGGGGTTATAACACTGCATACCATG10.6493506493506493No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCAAAGTACTCTGCGATGAAAC10.6493506493506493Clontech SMART CDS Primer II A (96% over 26bp)
ACATCCAACCAGGTCCCTCATCCTCCACCATCACCTGGTGACGTCTGATC10.6493506493506493No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC10.6493506493506493Clontech Universal Primer Mix Long (96% over 26bp)
CATGTGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA10.6493506493506493Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGAAGACCTTGGCGAAAGCCTGACGGGTTAAGAAGGTCATGGAAGAGT10.6493506493506493No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)