FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645079

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645079
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA149.58904109589041Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42.73972602739726Clontech Universal Primer Mix Long (96% over 26bp)
GGCACTCATCGTAGAAGCCATAGACCTGCGTGATCTGGCGACTCTCATGG32.054794520547945No Hit
ATTAGTAGAGACAGTGTTTCACCATGTTGGCCAGGCTGGTCTCGAACCCT32.054794520547945No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32.054794520547945Clontech SMART CDS Primer II A (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32.054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG32.054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC32.054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
GTCATAATCCCACAGATGGTAGCTTCGCCCCATTGGCTCCTCAGCCAAGC21.36986301369863No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTGGAATGGAGTGGCAGGATCATGGCTCAGTGCAGCCTTGAACCCCTGGG21.36986301369863No Hit
TGGGAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTC21.36986301369863No Hit
ACACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAAAC21.36986301369863No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCT21.36986301369863Clontech SMART CDS Primer II A (96% over 26bp)
GCACCAGGGCCCGCCCAGGGCGCCGCGCAGCACGGCCTTGGGGGTTCTGC21.36986301369863No Hit
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGC21.36986301369863Clontech Universal Primer Mix Long (96% over 27bp)
GTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGGGACGGC21.36986301369863No Hit
TTGTATTTCTGTGGGATCGGTGGTGATATCCCCTTTGTCATTTTTTATTG21.36986301369863No Hit
ACCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCG21.36986301369863No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG21.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.36986301369863No Hit
ATCTTAACCTGGATAAGTGCTATGTGATCCCTCTGAACACTTCCATTGTT21.36986301369863No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA21.36986301369863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGAAATCGTGGGCGATGACGATGCCGATCGTGCCGCTGCGCGCGATCGC21.36986301369863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGAT10.684931506849315No Hit
GTACAACTTTAAGGCCGCGACTCATCCACACCCAGAAGACGATGACAACA10.684931506849315No Hit
GGCTGACAGACTCCTCATACTGACAGGTGACCCTCTGGGACAAAGCTTCC10.684931506849315No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.684931506849315No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTTCGTTTATACCACTTC10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
AAATATTTGAATGGAGATGGGCAACAAAAGGGTATAAAGAGCTGGTGTTT10.684931506849315No Hit
TCCTTGATGCGCCCGCGCATCTCGGCGACGAGGCGCGCCTTCAGACCCTC10.684931506849315No Hit
TTATTTGTCTCCAAAGAAATGAGGAGGAAACTTTCCCAGGGAAAAGCTCC10.684931506849315No Hit
ATGTCTCCCCTTCATGAACAGTTTGCCTCAGCAGCAATTCTGTCTGCCCT10.684931506849315No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC10.684931506849315No Hit
ACCTAATGTAGATGATGGGTTGATGGGTGCAGCAAACCACCATGGCACAT10.684931506849315No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCTAAGAACTGGGGCTTTAACCTTGGAGCAAACCCTGGAGGTATTACACC10.684931506849315No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTAGCGTTTATACCAAAGCTACCCCA10.684931506849315No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTG10.684931506849315No Hit
CTTGCAAACTCATTCTATGAAAACCAGACAAGGACACAATGATAAAAGAA10.684931506849315No Hit
GTACATGGGAAGCAGTGGTATCAACGCATAGTACAAGGGAATCAGTGGAA10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAA10.684931506849315No Hit
AACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACTTAAGTCTAAGGCACTGAGCGTATCATGTTAAAGATGAGCGGGTGGC10.684931506849315No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCAACTGGGCTCCGACACCAGGCAGGCCGGCGTGCGCGCCGTGCCCTC10.684931506849315No Hit
GTGGTATCAACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTACACA10.684931506849315No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGCTGTC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCCAAAATGCTGGGATTACAGGCGTGAGCCACTGCGCCCGGCCTGTAGGG10.684931506849315No Hit
CTCTCACTATGAATTCTACATGCCTAAGCCTAAATTTGTCTCCTATATTC10.684931506849315No Hit
ATCCATAGAATGGGAGAAAATATTTTCAATGTATCTGCTATGGTTTGAAT10.684931506849315No Hit
GCTTGTAACCAGTGCCCTCTACTGAGGGTGGATCAACTGAGGTCAGGAGT10.684931506849315No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.684931506849315Clontech Universal Primer Mix Long (100% over 23bp)
CCTGCGCGCAGGATCGCGTAGAAGGGCCAGAAATACCATTCGGGCACGAT10.684931506849315No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTCTCTGAGTCTTTCAATTATGATTTCATCTGCCTGGAATCGACCCTTCA10.684931506849315No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCTCCCCCAACCCCCAATCTCCTGAGGGAGGAGAATTCCTAGGGACAAGA10.684931506849315No Hit
GGGAAGTATATGTGTAATAAATTGGCTAATGTCTTCCTAAAACTACTTCA10.684931506849315No Hit
TTGTATTTCTGTGGGATCGGTGGTGATATCCCCTTTGTCATTTTTTATCG10.684931506849315No Hit
TCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGT10.684931506849315No Hit
ACCCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.684931506849315Clontech Universal Primer Mix Long (95% over 23bp)
CCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTG10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
AAGTGAAATACCAATGATAGAAAATAGATGAAGAACTTACAAAAGTATCA10.684931506849315No Hit
CAGGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG10.684931506849315No Hit
GGTATCAACGCAGAGTACATTGGAAGCAGTGGTATCAACGCAGAGTACAT10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
ACACAAGGCAGCTGTCTCACACTTTACAAGCTGGTGCCTTCTGGACAGGA10.684931506849315No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGTTTTTAACACTG10.684931506849315No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
CTGAGGCTGAGATCATACTGATAGTTTTCCAGCCTGAAATTCAGATGTCT10.684931506849315No Hit
CAGTTATCCAAGTGGGAGAGGAGCGAGCGACCAAAGGAACCATAACTGAT10.684931506849315No Hit
TCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATTTGATCATTTATATTGGTCTTCAAGTTCACTGACCCTTTCTTATGAT10.684931506849315No Hit
GGACTACAGGCACCTGCCACCAGGCCCGGCTAATTTTTGTATTTTTAGTA10.684931506849315No Hit
GTCTATACATATATATATATGATAATTTGGTTCACTTGGGAAAATCAAAT10.684931506849315No Hit
GACCAGCCTGGACAACATGATGAAACCCCGTCTCTATTAAAAATACATAA10.684931506849315No Hit
GCACTCCAGCGTGGGTGACAGAGCAAGACCATGTCTCATTTTAAAAAAAT10.684931506849315No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATAATCCAGGGCTGTTGCGACAGAGACGCGGTCGTCGGCGTTCCAGA10.684931506849315No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGGGTTTAAAAC10.684931506849315No Hit
GCTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAA10.684931506849315No Hit
CCGCAGGCCGGTGTGACTTCTGCACCCTCGGTTCTGAGGGTACGGTGACC10.684931506849315No Hit
GCCCGAGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCC10.684931506849315No Hit
ATCGCGCAAGGTGCTGGACCTGATCCTCGACGTGCGGGCGCGCGGGCTGC10.684931506849315No Hit
CGCCTCAAGGTGCCACTGGACCTGAATGCCTTTGATGTGCAGGCCTCACC10.684931506849315No Hit
GTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGG10.684931506849315No Hit
GCGTTGATACCACTGCTTGCCCTGTACTCTGCGTTGATACCACTGCTTCC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph