FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT128.21917808219178Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64.10958904109589Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG32.054794520547945Clontech Universal Primer Mix Long (96% over 26bp)
CTCCACGAGTGTGACGGACATTACTGGAATGTTCAGGAAGATGATATGGA32.054794520547945No Hit
TGGTAGAGGAGAGCAACGTGCAGAGGGTGGACTCGCCAGTCACAGTGTGC32.054794520547945No Hit
GACACAACATACCAGAATCTCTGGGACACATTCAAAGCAGTATGTAGAGG32.054794520547945No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT32.054794520547945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCGTGTTGGGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGCCC21.36986301369863No Hit
TCGTTGAACAAACGAACCTTTAATAGCGGCTGCACCATTGGGATGTCCTG21.36986301369863No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
AAATGTAAAGGGTGGGGTTATGCTGTGATATTAATTTTCCTCAATAATGT21.36986301369863No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA21.36986301369863No Hit
CGCCTCCCGTGAGGGCCACATGCGCCTTGGCCGGGCCCGGGGCCACGCAG21.36986301369863No Hit
GATAACTGGCTTGTGGCGGCCAAGCGTTCATAGCGACGTCGCTTTTTGAT21.36986301369863No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
TAGTGAAACCCCGTCTGTATTAAAAATACAAAAGTTAGCTGGGTGTGGTG21.36986301369863No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGA21.36986301369863No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCT21.36986301369863Clontech Universal Primer Mix Long (96% over 25bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC21.36986301369863Clontech Universal Primer Mix Long (96% over 25bp)
ATCCAGAAAATACAAACAACTAGTACAACTCAACAATAAAAGATAACATA21.36986301369863No Hit
CGCGGTAGAAACGGAAGCGATCGCGCGCAGCGGCACGATCGGCATCGTCA21.36986301369863No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC21.36986301369863Clontech Universal Primer Mix Long (96% over 26bp)
GCGGTAGCGCGGCCTGCGCCGCCATCGTCGCCGCGCGGCCGTCCGCCATG10.684931506849315No Hit
CTTCCTCCTCTTCCTCCTGAGCCAGGGAGCTCTGACTGGAATCTGGAGAG10.684931506849315No Hit
CCACAGGGTCTCTGTGAAGGCCTCCACGAGGTGAGGGCCAGAAGCAGCCA10.684931506849315No Hit
GCTTGCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GAAAAAATATGAGTCCTGGTTCTTGTCTGAAACCTTCTACTGAGAAATTT10.684931506849315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATAACATGGGAAGCAGTGGTAACAACGCAGAGTACATGGGAAGCAGTGG10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTTTGGGAAGGCTGCAAATTGTAAGCTGGGTGTTGGGGCAAAGAAGAAA10.684931506849315No Hit
GAGCAGGATACGGTGCCCTTCCACCCCTATTATACCGCCAAGGATGCGGT10.684931506849315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCTAACAGATAGGCCCCTCAGCTGCAGGTCTGTTGGAGTTTGCTGGA10.684931506849315No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTAAAAAA10.684931506849315No Hit
GACTTATCAAAGACGTTAAAATGTCTTCTTAAAATATCTTGAAGGACTTA10.684931506849315No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAACACAAA10.684931506849315No Hit
CCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGG10.684931506849315No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
AGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGG10.684931506849315No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTA10.684931506849315No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
ACTACAACCTGAGCAAGCGCACCAAGGTCTACGGCTACTTCACCAAGATC10.684931506849315No Hit
ATTTCATTTGATTCTTACAACAAGCAGAAAAAACAGTGGGAAAGATTTTT10.684931506849315No Hit
ATCAACGCAGAGTACAGGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGTGACTTAAACAGCTTAAAGTTTAGTTTA10.684931506849315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGGACTCTGCGTTGAT10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
AGTAGTGAGGTAGCTAGAGGAGACATACAAAGGAAGAATTTTCTCGGGAT10.684931506849315No Hit
GTTTTTTCATGATCTTATTTAAGTAATTTGTTACTGTCAGGGATGCTAAC10.684931506849315No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACGCAGTGGTATCAACGCAG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTAAAGTAATATGAATATTATGTATTTGAGTAGCTCAGAATACCAATATC10.684931506849315No Hit
GGAGAAGAAGAGACGATGAGAAAAGTAACTGCTGCTCTGCCCAGCCCAAA10.684931506849315No Hit
GAGGTAAGTTAAATCACTTTTTTCTTGCTCATATGTCTTTTACATTACAT10.684931506849315No Hit
CCTTTTAATGTGTTGTTGAATTTGGACAGGCAGTATTTCATTGAGAATTT10.684931506849315No Hit
TCCATCAAGTGTGGATTTTTGTCGTCGGCATGATCGTCGGCTTTGCGGCG10.684931506849315No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
ATATAATTTTGTGAGAATTTTTTTAGCTCATTTCATCATGGACCTCTGAA10.684931506849315No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTTGTACCCTTTGCTCCCTTTCTTTCTTTCCATCTTTCTTTCTCTCTT10.684931506849315No Hit
GCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
TTCAAGCAGTTCTCCCTACCTCAGCCTCCCGAGTAGCTGGAATTACAGGT10.684931506849315No Hit
CATGGGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.684931506849315Clontech Universal Primer Mix Long (96% over 25bp)
CCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGTC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCCATTTACTCTGCGTTGAAACCAAAGCTTCCAAT10.684931506849315No Hit
GAGTACATGGGAAGCAGTGGTATCAACATAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
GGCGACCACGGGCCCGCGCCTTGCTGTTCTTGTCGCAATCGCCGACCGCC10.684931506849315No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACAGGGCAAGCAGTGGTATCAACG10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTCATGAGGATGTAAATTTCATTCAATAATAAGATAAATGGCCGGG10.684931506849315No Hit
GGGAAGCAGTGGTATCAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.684931506849315No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.684931506849315No Hit
ATATTACTGTGTTGCCCAGGCGGTCTCAAATCCCTGATCTCAAGCAATCT10.684931506849315No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10.684931506849315Clontech SMART CDS Primer II A (96% over 26bp)
GTCCTCGCAGGGCTCCTCAATCCCCAGGCCATGGACCGGTACTGGTCCAT10.684931506849315No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA10.684931506849315No Hit
GTTCTGCACATAATCATGATTGTAGCAGTCAGGAAAGAGGTAGAAGCCCC10.684931506849315No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 23bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTACGCAGAGTACATGGGAAGCAGTGGTAT10.684931506849315Clontech Universal Primer Mix Long (95% over 22bp)
CCTTTAAGTTTCAGCTTTGCAACCATACTCCCCCCGGAACCCAAAGACTT10.684931506849315No Hit
GTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATAAT10.684931506849315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10.684931506849315No Hit
ACCACAGACCAGGTAATTTGTAAAGAATAGAAATCTACTTTTCATAGTTA10.684931506849315No Hit
GGACTTGCCCGCGCCGGGCGGTCCCGCCAGCGCGACGAGGCGGCGGCCCG10.684931506849315No Hit
GGCACACACCAGTACACCTGGCTAAGTTTTATGTATTTTTAATAGAGACG10.684931506849315No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTGTAAAGGCCTCTTTGAGGAAGAACCTGAGCGAATATCCATGAGAG10.684931506849315No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCTTCCCATGT10.684931506849315No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTAA10.684931506849315No Hit
GAGTACAGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTCTCTCCTGCGGCCCAGCCTCCCTCTCCCTCCTCCATTCCTCTCTCCC10.684931506849315No Hit
GTGAGAGACACATCAGAGCCCTGGGCACTGTCACTGCTTGCAGCCTGAGA10.684931506849315No Hit
CGGTAGCGACGCGGCCCTGCCCGGCCTTTGACCATGACCATCGACCGCGA10.684931506849315No Hit
GCTTCCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTACTCTGCGT10.684931506849315Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATTGGGACGCAGTGGTAACA10.684931506849315Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGGGCGGGCGGT10.684931506849315No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAC10.684931506849315Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph