FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length140
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA106.211180124223603Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42.484472049689441Clontech Universal Primer Mix Long (96% over 26bp)
TCCCTGAGAAGCCCTTTCCAGTAGGGTGGGCAATTCCCAACTTCCTTGCC31.8633540372670807No Hit
GAGTACATGGTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT31.8633540372670807Clontech Universal Primer Mix Long (96% over 26bp)
GTATTTGGGATCATATACTTGCCGCCCAAGCCCATACACACTCATTCCTT31.8633540372670807No Hit
GTACTGTTCAAAAAGGAATGCCCCACAAGTGTTACCATGGCAAAACTGGA21.2422360248447204No Hit
ATGGTAACCATACTCTATTCCATAACCCTCATGTGAATCCACTTCCAACT21.2422360248447204No Hit
GTCTTAAGCAAGCCATTCCTGCTTTCTGGGCCTGGCTCCCATGGGCCATT21.2422360248447204No Hit
CAGCAGGGATTCGATGTCGTCGCGGATCCAGCGGCTGTCCAGCGCCAGGC21.2422360248447204No Hit
GGGCTGGGATGCCCACACCACCTGCCAGGCCCTCGGGGTGTGTGGGACCA21.2422360248447204No Hit
CCCCTGACCAGCCCCACTGGGAAGGCAGCAGGATGGCAGTGGCCAGTGGG21.2422360248447204No Hit
GGTGAACAGCATGATCCTCTTATGACTCATCTTGAATTGGACATCACTAA21.2422360248447204No Hit
GTGGCGAGGTTGCGGATCAGGGCGCGGATGGGAACACACAAGAGGGGACG21.2422360248447204No Hit
TCCAGGAGGAGAATGAAGATCAGATTTTTGCAATCCTCAGATCGCTGTTT21.2422360248447204No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.2422360248447204Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTAATGTTAGCGGTTAGGCGTACGGCTAGGGCTATTGGTTGAATGAG21.2422360248447204No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA21.2422360248447204Clontech SMART CDS Primer II A (100% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
ATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGC21.2422360248447204No Hit
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG21.2422360248447204Clontech Universal Primer Mix Long (96% over 27bp)
GACCTGAACTTCGGCGGCAACGTCAACGACTACTACGACCCCGACAACAG21.2422360248447204No Hit
TCCTGGAGAGGCAGGCGCAAGGGCTTGTCAGTTGGACGAGTTGGTGGTAG21.2422360248447204No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
GTTGTCCATTCATTTAAAAAGCAAAGTCACTAGGATGGTAGAAAAGTGAT21.2422360248447204No Hit
GCTTTTGCACATGCACGTTTGCAATCCTCCCTGCTAACAAAGTTATTGTC10.6211180124223602No Hit
CGTCAGATGTGTATAAGAGACAGGCTTCACAATGATAGGAAGAGCCGACA10.6211180124223602No Hit
CAATGTTACTATCAGCATGATCTCTCAGTTCTTTCAGCCATCGCTCTACA10.6211180124223602No Hit
GTACATATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTAAC10.6211180124223602No Hit
CCCGACCTTCGCCCAGCTCGCTGACCCGGGGCAGCTGGCGGGCGAGCTGC10.6211180124223602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGGTGAAAAGA10.6211180124223602No Hit
GTACATGGGGTGCCCTGCGCGTGAGTAGCTGCAGCGGCAGAGGCAGCATC10.6211180124223602No Hit
GGGAAATATCTCCTATCTTGGAAGATAATTGGGGTTTGCTAGGGAATGAG10.6211180124223602No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTCATCTAACCTTCCAATGTCTGATGTTCCTGCCAAATTCCTGCCTGA10.6211180124223602No Hit
TCCCAGTGCTTGAGGAGCGGCTGGTCCAAGCCCCAGTGCTCCACCCTGCA10.6211180124223602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTCGGTGTAAATA10.6211180124223602No Hit
GTGCCCTTCGTCCTGGGAAACGGGGCGCGGCCGGAAAGGCGGCCGCCCCC10.6211180124223602No Hit
GTATCAACGCAGAGTACATCGGACAGCCTAACGCTCTTCGCTGTCGTTTG10.6211180124223602No Hit
CTTGGCATACTTCTCCTTCACTGCAATGTAATCCTGCAGGGAAATGTCAT10.6211180124223602No Hit
GAAGGGCAAAAGCTCGCTTGATCTTGATTTTCAGTACGAATACAGACCGT10.6211180124223602No Hit
TACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCG10.6211180124223602No Hit
TATTAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTCTAAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCTGGATGTTGTA10.6211180124223602No Hit
GTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGGTA10.6211180124223602Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA10.6211180124223602No Hit
GCCATTCTCCTGTCGGCGACCTTGGGCCAGCGCTGTCATCCATGGAGCAC10.6211180124223602No Hit
CTCCGTGACAATGAATTCTTCTGTGAAGTGGATGAAGACTACATCCAGGA10.6211180124223602No Hit
TGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCG10.6211180124223602No Hit
CATCTAGATATTAGAAAATCATCTAGGGTCGCTACAATTTTTTTATATAC10.6211180124223602No Hit
TTTCAAACAGGAGCCAATGGTGCATCCAGCTCTCAAAGACCTTCCAGTCT10.6211180124223602No Hit
CCACAAACCATTAATGCTTAGTCAGCCCCCTGATAATGGAATTATCCTGA10.6211180124223602No Hit
TCTCAGCGAAGTACACCTTCTTGCTGCAGCGCGGGCACGTGTTGGGCTCC10.6211180124223602No Hit
GTACATGGTAAGCAGTGGTATCAACGCAGAGTACATGGAAGCCTGTCTCT10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG10.6211180124223602No Hit
CCATTTTACTGATGTCACCCAACCAGTATGTGACGTTAGGGACAGCGACA10.6211180124223602No Hit
GTCTCGCGCTGCGGCCATGGCGGCGGGTGAGCCAGGAGACCGCCGAGCGC10.6211180124223602No Hit
CTACAAGACAGCGCAAATGTGGCTTTTGTAAGTCAAATAGAGACAAGGAA10.6211180124223602No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAGCGCAGAGTAC10.6211180124223602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAG10.6211180124223602No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GTTTTTCATCATTCAGCTAGAAAGTGAGAAGTTTTATCTTCCATAGTCCT10.6211180124223602No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
CTCCAGCACTACTGCCAGGGTCATCACAAACCAGTACAACAACCCAGCTG10.6211180124223602No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGCGTCACACAAAAACGGAACAGGGCTGACGCCGCTACAAATATAG10.6211180124223602No Hit
CCTCGAAAGAGTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCG10.6211180124223602No Hit
CTACACATGCAATGAGGACAATATTCTGCTAATACAATTGACTTGCTGCT10.6211180124223602No Hit
ACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATAACCGATTAAA10.6211180124223602No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT10.6211180124223602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATGAA10.6211180124223602No Hit
TCATAGTAATACCCATCATAATCGGAGGCTTTGGCAACTGACTAGTTCCC10.6211180124223602No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC10.6211180124223602Clontech Universal Primer Mix Long (96% over 25bp)
TTGCATAGGGGGAGGAGCCAATGTCTCTGACGTCTCACCACCCTCCAACA10.6211180124223602No Hit
TCCCCCATTATTCCTAGAACCAGGCGACCTGCGACTCCTTGACGTTGACA10.6211180124223602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTATAAAA10.6211180124223602No Hit
CAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGC10.6211180124223602No Hit
CTCCCTGCTTCAGGCGGAAGAGCTGGAGCACGAAGATGAAGAAGGCGATG10.6211180124223602No Hit
ACCAATAGCAGAAGGAAATTTGGGAAATTCACAAATAAGTGAAATTTAAC10.6211180124223602No Hit
GAACAGCTCAAGCGCGTCGAGGAACGGGGCCACGGCCTCGGGGCCGCAGG10.6211180124223602No Hit
CACGCAGCAATGCAGCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGA10.6211180124223602No Hit
CACTTCTGGGAGCCCTCTATGAGGTGGTTCACGGGGATGCCCAGGCTGCT10.6211180124223602No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.6211180124223602Clontech Universal Primer Mix Long (100% over 23bp)
ACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGATACCACTG10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GAGAGGGGCTGACCGGGTTGGTTTTGATCTGATAAATGCACGCATCCCCC10.6211180124223602No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GTGACTGTGGTTCCCATTATCGAACGCAATGTCAATCTGCACTGTACACG10.6211180124223602No Hit
GTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACG10.6211180124223602No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GGTGCGATCTTGACTCACCACAACCTCCGTCTCCCAGGTCCAAGAGATTC10.6211180124223602No Hit
TCCTTCTCCAGCTCCTGCAGCTGCCTCTGTTCTTCTTCAACCAGGAAGTT10.6211180124223602No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTAC10.6211180124223602Clontech Universal Primer Mix Long (96% over 28bp)
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTAAA10.6211180124223602No Hit
GTGTTGGGCCTCCCCTTAGGCATGTCCGATTGCTCGAAGTGCTCCAGCTC10.6211180124223602No Hit
GCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAG10.6211180124223602No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGCTGTCTCTTATACACATCTCC10.6211180124223602No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10.6211180124223602No Hit
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGAATCAACGCAGAGTACA10.6211180124223602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TCTTAGTACAATAACTAAATTGACTGTTCAGGAAACAAAGAGTTTTTTTT10.6211180124223602No Hit
AATATGTTCTGAGAGTGGACCACTTCAGGTCTCTTCCTATTTCAAGTATA10.6211180124223602No Hit
TTCCACTTCTGGCATGACAGCATGAGGAGCTCCATGGACATGATCCCCAA10.6211180124223602No Hit
ATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAACAT10.6211180124223602No Hit
TATTAAGGTTACGTGAAAGACTTGCTCAGTGTGATCTGGGGCTCCCTCTT10.6211180124223602No Hit
TTCCCAGACTCTGCGTTGATACCACTGCTTCCCAGACTCTGCGTTGATAC10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT10.6211180124223602No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATTTACTCTGCGTT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGA10.6211180124223602No Hit
GTCTCAACGATGCATGGATAAACATCAACAGCAAAGGAAAAGTAATGCCA10.6211180124223602No Hit
CGTAGTTGGATCTTGGGAGCGGGCGGGCGGTCCGCCGCGAGGCGAGCCAC10.6211180124223602No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGAGCTG10.6211180124223602No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGACTGGAGCCAGCA10.6211180124223602No Hit
CCCAAATAATTATTAGAACCTCCTCCCCATGTGCCAGCTCCAACCTCTGC10.6211180124223602No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCTGGCGCAGACCGGCACGCCACAGCGGCGGGTGCACCTTCATGCTGT10.6211180124223602No Hit
CACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGCCGGAGAGGGGCTGA10.6211180124223602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCCTGTCT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GGGCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCC10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6211180124223602No Hit
GCTTCCCATGTACTCTGCGTTGATACAACTGCTTCCCATGTACTCTGCGT10.6211180124223602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGGAATAGGTTCCAGCAGCTCAGGCTCCTTCCCATTGGTTCTCACAAAGT10.6211180124223602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)