FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT42.484472049689441Clontech Universal Primer Mix Long (96% over 26bp)
CAGATGGGCACTAATAAAGGAGCCAGCCAGGCAGGGATGTTAGCACCAGG31.8633540372670807No Hit
GTCCATACCCACCAGACACAGAACTGAACACCCACACACCAGTTTTCAAA31.8633540372670807No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.2422360248447204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTGCTTCC21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
TCATGACCGGGCGCGTTGCGCCCGTTCGCGTTGGGGGACGCGATGCTGTT21.2422360248447204No Hit
ATCACCACCTGCCCCTTGGGCAGCGTGACCTTGAGCATGAAGTGCCCCGG21.2422360248447204No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA21.2422360248447204No Hit
CTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGGACTCCT21.2422360248447204No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.2422360248447204Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGTAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.2422360248447204Clontech Universal Primer Mix Long (95% over 21bp)
TCTTTGATATCAGTGATGAAAGTAGTGGAGAAGGAAGTGGAAGTGGCTGT21.2422360248447204No Hit
GACCCAAGCTGGCCACATGGCGGCAGCGCGTGGAGGCAGCAGTGGGGGAG21.2422360248447204No Hit
AGACAGGGGCGAGGGCCCTGTGGAGCTGGCCCATCTGGCCGGGCCCGGGA21.2422360248447204No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
GCGGTGAGGAGGGCGGTGTGGGAGCTGAGGCTTCTTTTCAGCCTGGCAGC21.2422360248447204No Hit
CTTTGGCAAAGCCACCTTCGGGTGGCTTTTTTATTGCTCCGCCATCGTTC21.2422360248447204No Hit
CTGTGGTGTTCTATGGTACCGAGAAAGACAAAAATTCAGTGAATTTTAAA21.2422360248447204No Hit
GCCCAAATATCCTCGGAAGAGCGCTCCCAGGAGAAACAAGCTTGACCACT21.2422360248447204No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT21.2422360248447204Clontech Universal Primer Mix Long (100% over 23bp)
TCGTTGTCCTTGCAAATCATCAGCTGAGCAAGGAGGAAGCGATTTGCCCC21.2422360248447204No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
ATTTAAGCCATCTCCTGCCACAAGCAAAGGGATGGGAGACCAGTATACCA21.2422360248447204No Hit
GTCCTGATTGTTGCTGCTGGTGTTGGTGAATTTGAAGCTGGTATCTCCAA21.2422360248447204No Hit
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG21.2422360248447204Clontech Universal Primer Mix Long (96% over 26bp)
GGGAATAGGTTCCAGCAGCTCAGGCTCCTTCCCATTGGTTCTCACAAAGT21.2422360248447204No Hit
GTACATGGTAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAATAAAAAA10.6211180124223602Clontech Universal Primer Mix Long (95% over 21bp)
GAACTCCTGAGCTCAGGCAGTCTGCCTGCCTTGGCCTCCCAAAGTGCTAG10.6211180124223602No Hit
GTCCCTCTTGAGGAGGCTGCACAGGAGTACCAGGAGAAGCTCCAGGTGGC10.6211180124223602No Hit
TGCGCAGCTCGTGCGCGGCATCGGCGATCAGGCTGCGCTCGCGCGAGGTG10.6211180124223602No Hit
CCGCGGTCCTAATCCATTATACCTAGCTGCGGTATCCAGGCGGCTCGGGC10.6211180124223602No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10.6211180124223602No Hit
CATGTACTCTGCGTTGATACCACCGCTTCCCATGTACTCTGCGTTGATAC10.6211180124223602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCAGTCACTCTCTTCGCTCTTTTCCCTCGATCTCTCCCTGGTCAGCCAGC10.6211180124223602No Hit
GATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGAT10.6211180124223602No Hit
TAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGG10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACAAA10.6211180124223602No Hit
CTCTTAGCTGAGTGTCCCGCGGGGCCCGAAGCGTTTACTTTGAAAAAATT10.6211180124223602No Hit
ATGTTGAGTCTGGCAGTCTTAAGTCAACTTTGACAGGAAATAAAGTGTTT10.6211180124223602No Hit
CCAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG10.6211180124223602Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
TCTTTAAAGTTGTCCTTATTGGAGATTCTGGTGTTGGAAAGAGTAATCTC10.6211180124223602No Hit
GCTTCACCTGGGATGTCTGAAAGGCCAATGGGAAGCATTGGCTGGTTCTC10.6211180124223602No Hit
GGCAGCTGGAGGGAAGTCCCTGAAAGTGCCTCTCTACCCAGCAGAGGGGC10.6211180124223602No Hit
CTCCCACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGG10.6211180124223602No Hit
ATCTGTGACCGGTCAAACATGGCAAACACATTGGATGTTGCACACTGAGG10.6211180124223602No Hit
GCAGTGGTATCAACGCAGAGTCTGGGAAGCAGTGGTATCAACGCAGAGTC10.6211180124223602Clontech Universal Primer Mix Long (100% over 23bp)
CCATGACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GGCTCACTCTTGTCCAGGTGGGAGTGCAGTGGCATGATCATAGTTCACTG10.6211180124223602No Hit
GCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCCCCCGG10.6211180124223602No Hit
AGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGAT10.6211180124223602No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTTTACCCAATCGTACCC10.6211180124223602No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT10.6211180124223602Clontech SMART CDS Primer II A (96% over 26bp)
GTATGCACCTGGGCCTGTGCAAATCCCGGCAGCCAGAGCCAGAGCAGGAG10.6211180124223602No Hit
CGGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGC10.6211180124223602No Hit
GTAATCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.6211180124223602Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GACTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTGCTCCGTCTTAG10.6211180124223602No Hit
GGATAAGAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGG10.6211180124223602No Hit
GTCTACGACTCACTGCAGGATGCAGCTCTGGCCCCCGAGTTCCTGGCCGT10.6211180124223602No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT10.6211180124223602Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGA10.6211180124223602No Hit
GCCACAAGCCGTGCTACGCCACCCTGTTCGGACCCAAAGGCGTGAACATC10.6211180124223602No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTTTAGTTCCCTCTAGCAGACTGGATCTTTGACTACCAACCTCAAATAG10.6211180124223602No Hit
GTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACAG10.6211180124223602No Hit
GCATGACTATGAGACGATAAAATGTCCATCCTTATATATTTTCGTATGCC10.6211180124223602No Hit
AATCGTGTGACCGCGGTGGCTGGCACGAAATTGACCAACCCTGGGGTTAG10.6211180124223602No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GACGTGGACTGTTTCGGGGGCTCATTCAACTCCTGTTTCTCCTGAAGCAT10.6211180124223602No Hit
CTGGAGAACCTGCGCATGAAGCTTCCCAAGCCTCCCAAGCCTGTGAGCAG10.6211180124223602No Hit
GTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTGGTGGTGC10.6211180124223602No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGCTTCC10.6211180124223602Clontech Universal Primer Mix Long (95% over 22bp)
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC10.6211180124223602No Hit
GCACTGCTCTGTTTATAATTGTTAATATTAGACAAACAGTAGACAAATGC10.6211180124223602No Hit
CCTAGAATGAAAAAAGGGCAGGATGTAGACCTCAGCTCTCCATAAGACAA10.6211180124223602No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGC10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGGCCACCCCCTGCTTCCCAGAGTGAAACCTTGTGCCTGGTGACCA10.6211180124223602No Hit
CCCTATTAGTGGGTGAACAATCCAACGCTTGGTGAATTCTGCTTCACAAT10.6211180124223602No Hit
GACAAGAAGGAGACCGTCTGGCATCTGGAGGAGTTTGGCCAAGCCTTTTC10.6211180124223602No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGTAAAAAAAAAAAAA10.6211180124223602No Hit
CCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAA10.6211180124223602No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG10.6211180124223602No Hit
ACGCAGAGTAATGGGAAGCAGTGGTATCAACGCAGAGTAATGGGAAGCAG10.6211180124223602Clontech SMART CDS Primer II A (96% over 26bp)
CCCCCTCGCCCGGCACGCACCGCACGTTCGTGGGGAACCTGGCGCTAAAC10.6211180124223602No Hit
GTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGGGGCGTGC10.6211180124223602No Hit
TCTCTAACAGCAGTAATATTAATAATTTTCATGATTTGAGAAGCCTTCGC10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATAAAAA10.6211180124223602No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
ATCCTTGCATCAGCCGCTGGAGCAAAAGCTGGAGTTAGGCCCCATATCAA10.6211180124223602No Hit
CGAATACAGACCGTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGG10.6211180124223602No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTTGTA10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGAGGTTTCCCGTGTTGAGTCAAATTAAGCCGCAGGCTCCACTCCTG10.6211180124223602No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAA10.6211180124223602No Hit
GGGTAGTAGCCGGTGAGGAGCAGCTCGCCCGCCAGCTGCCCCGGGTCAGC10.6211180124223602No Hit
GGGAGCCTGAGAAACGGCTACCACATCCAAGTAAGGCAGCAGGCGCGCAA10.6211180124223602No Hit
ACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCTTC10.6211180124223602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTGCAAAACATCTACTCTGCGAT10.6211180124223602Clontech SMART CDS Primer II A (96% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGA10.6211180124223602Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACATGGGGCTCTTCCTGTCTGTACCAGGGCGGCGC10.6211180124223602No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTACCATGTACCTGTCTCT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GTCACCTCCGTGGCAGCCCTTTCTACTGTCTTGCAGTCCTTCTATATCGA10.6211180124223602No Hit
TGGGAGAGCTTCAAAACAGCCCGGCGGCCTCGCCTCGCACCCCCAGCCAG10.6211180124223602No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.6211180124223602Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAGACGTTCG10.6211180124223602No Hit
GTGGGGTTTCTGAGTCCCTTCAACATGATCCTGGGAGGAATCGTGGTGGT10.6211180124223602No Hit
GTACATGGGGTGGTTATTGAGTTAAGCCTGGCGAGTCGAGGTGACCCGGG10.6211180124223602No Hit
GGATTGAGAACAGGTTTCCAGATGAAGCTACTTGTATGGGCTTCTGCGCA10.6211180124223602No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGCCATGTACTCTGCGTTGAT10.6211180124223602Clontech Universal Primer Mix Long (96% over 28bp)
TTTCCTGTGTTGTCACAGTGACAAACTTAAGTAGATACAAAATACAAAAG10.6211180124223602No Hit
GCCTTGGCTTCTGGAAGTCTAGAAATATAAATGTCTCGCTTGGTGCCTGT10.6211180124223602No Hit
GATCCGTAATATAGGCCTCGCCCGATGTGTAGGAAGAGGCAGATAAAGAA10.6211180124223602No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAGTAC10.6211180124223602Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACTTTTTTTTTTTAGGTGATGAGGGCTCCAGTTCATGTTCATTAAA10.6211180124223602No Hit
CAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGAAGCTCGCCGCCCTC10.6211180124223602No Hit
TCCCAGCGCGGCGCTGGCCGCCCGTGACTTCCCGGACGGCGTCGGCTCGG10.6211180124223602No Hit
CTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAA10.6211180124223602No Hit
GCTTCCCCAGTGTACATATTGTATCTGTGTAACATTTTGTATATTCCAGG10.6211180124223602No Hit
GACCCAACGCCTCTAGCGCAAGATGCTGGAGACCCTGCGTGGCTCGGACC10.6211180124223602No Hit
TAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGCTC10.6211180124223602No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCT10.6211180124223602Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACACATCTGACGC10.6211180124223602No Hit
ACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGAATGGGGTC10.6211180124223602No Hit
GACTTAAGCAATTCTCCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGG10.6211180124223602No Hit
GAATTAATGTGCGTATTGAGCACATTAAGCACTCTAAGAGCCGAGATAGC10.6211180124223602No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)