FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA108.849557522123893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT97.964601769911504Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC32.6548672566371683Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG32.6548672566371683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG32.6548672566371683Clontech Universal Primer Mix Long (96% over 26bp)
GTATCGAGCAAGTCGTATTGCGTACGCTTCTGGCGCGAGCGGCGCGCTGG32.6548672566371683No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21.7699115044247788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.7699115044247788No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG21.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
GTCTGCCTTCGCTACGGAGACACTGCGGAGAGCATTTATCCGGCAAGCCG21.7699115044247788No Hit
GCCGTATCCTGTCCAAGCTACATACTGTCCAACCCAAGGGCAAGATCACC21.7699115044247788No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.7699115044247788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCGCCCCGTGTCGCGGCGGCACAGCCAGTCGCACGACGTCAGCGAAGAC21.7699115044247788No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC21.7699115044247788Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA21.7699115044247788Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAAGAGGTGGTAAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCC21.7699115044247788No Hit
TTCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTATA10.8849557522123894No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
GCCTCATGGCGCAGAAACCTCTCAGCACCGCGGCGGCTGAACGCATGAAC10.8849557522123894No Hit
TCGGAACGCAATATCGGCAACACTGGCATCCACTCGCCGAGCTTGATTTC10.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGAA10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCTCTGCAACCTTGCGCTTTGCCGGCGGCCGCGAGCTCTCGCTGGGCG10.8849557522123894No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA10.8849557522123894Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTAACAACGCAGAGAACAT10.8849557522123894Clontech Universal Primer Mix Long (95% over 22bp)
GCAACCTCCGCTTCCTGGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGA10.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGAAGCAGTGGTA10.8849557522123894Clontech SMART CDS Primer II A (100% over 26bp)
CAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGT10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CCGTAGCTAGCGTCTGGCCTGAGAACCTCGGCGCTCCGGCGGCGCGGGCA10.8849557522123894No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC10.8849557522123894Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCATACAGGCTGTCCCTTTGCTTCTTGGACATCCTCCCAAACTTCACAGC10.8849557522123894No Hit
GGTGAGGCTGTCAATGAGGGAATCCCGCATGCTGGTGGCAATGGTGCTAG10.8849557522123894No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTT10.8849557522123894No Hit
GGTAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8849557522123894Clontech Universal Primer Mix Long (95% over 22bp)
TCCCAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.8849557522123894Clontech Universal Primer Mix Long (96% over 27bp)
AGGGTGGGCTGTCTCTCCACCTCCTCACATTATGCTAACAGGGACGCAGA10.8849557522123894No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCGACGGAAGGCGACGAGGAGGACCAACTACCCCTCGCCCACCGCCGC10.8849557522123894No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGGTCCTGGTCATCGCGTATG10.8849557522123894No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCTTATAC10.8849557522123894No Hit
AAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
GGGGCGCCGCGCACCGAATCCATGATCCGCGCGAAGCCGGCGATCGAGTC10.8849557522123894No Hit
GCATGGCTTTCACCGGCAAGTTCGAGATGGAGAGTGAGAAGAATTATGAT10.8849557522123894No Hit
GCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8849557522123894No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTGCGTTGTTACCACCCCAAGT10.8849557522123894No Hit
GCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCTTTC10.8849557522123894Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTA10.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
CGCTCTGGCGTGAGCAGCACGCGGCGCGCCGCGTCGTGCGACATGGCCGT10.8849557522123894No Hit
GCGCAAGGCCTGATATGCCGCGCCGCCCCTGGCTGACCGGCATGGCCGTG10.8849557522123894No Hit
CGTCTGTTTCTTTCACTAGGAGATGGAGAATTTTCTGAAATGGTTCCAGG10.8849557522123894No Hit
GATTCAGGCTCTGACTCTAACATTGACTTTTTGGGTGATTTTGAGCAAGT10.8849557522123894No Hit
ACGCAGAGAACGCGCCACATATCTTCTTCTGGGCTTTTCGGCTCGGCTTT10.8849557522123894No Hit
CATGGGAAGCAGTGGTATCAACGCTAAAAAAAAAAAAAAAAAAAAAAAAG10.8849557522123894Clontech Universal Primer Mix Long (95% over 21bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGCACCGAAGTGCGACGCGTCAATCAGGCGATGGGGAATCAGGAAA10.8849557522123894No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTGGGCAGAGGTATCA10.8849557522123894Clontech SMART CDS Primer II A (100% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
AGCAGATACTACCACCCCTACACCTACAGCCATCTTGGCTCCTGGTTCTC10.8849557522123894No Hit
ACGCAGAGTACATGGGAGCAGTGGTATCCAAAAAAAAAAAAAAAAAAAAA10.8849557522123894No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8849557522123894No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.8849557522123894Clontech Universal Primer Mix Long (96% over 26bp)
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAATGCAAAAAAAAAAAAAAAAAAAAAAA10.8849557522123894No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTCTAA10.8849557522123894Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph