FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112
Sequences flagged as poor quality0
Sequence length140
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAGAATGGTTTTTAAGCAGTAATGAACAGGAATGCCTTACTCTAAA32.6785714285714284No Hit
GCAGAATGGTGAACAGTGTTCTTGTGTTCCTGGGCATTTGGGTTATAGGT32.6785714285714284No Hit
GGAAGAGAAATGCTTCCATATGCTGAGATGATTAACATCTACCATGATGG32.6785714285714284No Hit
GCAATAGAGTGAGACTGTCTCAAAACACGAACAAACAAAATAAACAAATA32.6785714285714284No Hit
CCTGTGTTTCTTGTGCCAGGTTCTATTCCAGGCGCAGGTGGGCATCAGTG21.7857142857142856No Hit
GCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAG21.7857142857142856No Hit
ATATAAAATGTAAGTTATAAGATGAATATGTTCTGAGGGTCTGATGTAAA21.7857142857142856No Hit
GTTATGGAATAATTTGAGTTTGGATGAACATAGATCAAAAACATAACTTC21.7857142857142856No Hit
GAGTTTCGCTCTTGTTGCCCAAGCTGGAGTGCAATGGCAATAGCATGATC21.7857142857142856No Hit
ACCCCAGAGGTCAGGGCCTCTCGCCCAGCTCTGGCTCTGATGTCCTGGAG21.7857142857142856No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA21.7857142857142856Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CACCTGCCTTGGTCTCCCAAAGTGCTAGGATTACAGGCGTGAGCCAGCAC21.7857142857142856No Hit
GCATGAAGCAAACAGGATGAGGAAGAGGTGACATGAGGGACAAATGCACA21.7857142857142856No Hit
GACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCAAA21.7857142857142856No Hit
CATTGATTCCCTTCGATTCCATTCAATGATTCCATTTGATTCCAGTGGAT21.7857142857142856No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC21.7857142857142856Clontech Universal Primer Mix Long (96% over 25bp)
GTGCATGAGGCTATATAACTTGCTTCTGATCTTCAGGACTTAACAGGTGT21.7857142857142856No Hit
CATTTTGATGCACCACCAGTGTTAAGCACCTCTAAGATAGAGTCATGAAT21.7857142857142856No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
ATTTCAGGTGATTTTTACAACTATGTGATAACTTAAATAACTTATGTTAC10.8928571428571428No Hit
TATCAGACCAACTACCTTTTCACTAGAACTCTCTTCCTGCACTGTACCCC10.8928571428571428No Hit
TCCCAGAGATTCTTGCTTGTTTTATCTTTTTGTTATTTTCAAAGAATGTC10.8928571428571428No Hit
CTTGTAAGTTTTCTCTCCAGTGTGAATTCTTTTATGTCTTGCCAGCTGAG10.8928571428571428No Hit
GTATTCTACACTAATAGCAATAGCTGTTTATGAGCACTTATGTGTATACT10.8928571428571428No Hit
AACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAACC10.8928571428571428No Hit
GAGTTTGCAGTGTTTCCAAAGAGCATTAGGAAGCTATAAGTAAACAGAGG10.8928571428571428No Hit
GTAACAACTAGGCAGAAGCATTCTCAGAAACTTATTTGAGATGTGTGGAC10.8928571428571428No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.8928571428571428No Hit
CTCCAATTTTAGTTCATCCTGACGACCTCACAGTGGAGGACCTTCCTATA10.8928571428571428No Hit
AAATTGTATAAAACTCTTTGGTTCTCTTACTTACTATAATAGATGGTAGA10.8928571428571428No Hit
GTAGCAAATTCTATTCATCTTAATGGATTCTTTTCTTGGAAGATGTCAGT10.8928571428571428No Hit
GAATGACTTGACAAGGATATTGTGGCCCTCATGACTAGAAGGGCATATGA10.8928571428571428No Hit
CTCCGCTTCCCAGGTCCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGC10.8928571428571428No Hit
GCCACACGGGTCAGCACTCCACCAAATTTGTTGGAAAAAAATAAACAATC10.8928571428571428No Hit
GGGCCTCGATGGCCCCCGGCCCCACGTCGTCGCTGGGGATGAGACGCGCG10.8928571428571428No Hit
GAAAGTCAAGGGAGCAGGACTTGCTGCAGATGATGTGTGATCCAGGGGCT10.8928571428571428No Hit
GCAAGGGAAAGATGAAAAAATATAACCAAGCATAATATAGCAAGGACTAA10.8928571428571428No Hit
GTTCAACTCTATGAGTTGAATGCAAACATCACAAAGAAGTTTCTGAGAAT10.8928571428571428No Hit
GGGATACAGGCGTGAGCCACTGCACTTGGCCTAATATGTAATTATTAAGA10.8928571428571428No Hit
CCACAGAACCACTAGTTGGTTTTCAGTGTTGTAACAAGACCTCCCCCTGA10.8928571428571428No Hit
TACCAGCACTGTTTGTTAAAAAGAGTATTCTTTCCCCATTGGCTGGTCTT10.8928571428571428No Hit
CCGAAGAAATGCTCGCGGGTATATGCGCCGCCGAAGGCCTTGCAGTTCTG10.8928571428571428No Hit
GATATGCTGAGAATGGAAACCAGACCGCCCATTGGTCTCCCCCTCCGGCC10.8928571428571428No Hit
GCTGGCAGACCATTAGGCTGTTTTAAGTCCAGCAGGGTTATGAAACAGGA10.8928571428571428No Hit
GGATCATGTAGCGGATCGCGCGGACCGCCTCCGTGTCGCCGTGGATGCCG10.8928571428571428No Hit
CCTGGGCGACAGAGCAAGACTGTGTCTCAAAAAGAAAAAAATTTGTTCAA10.8928571428571428No Hit
CTGTTACCTTAATTAGAAAGACTAAATAATGAAACAATCAAAAATAATAA10.8928571428571428No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG10.8928571428571428No Hit
GTCATGACAATACTTAAAAAAAAGCTAGAGTATTTTAAACCAAATCTAAG10.8928571428571428No Hit
GTGCCATTCTGGGTAAAAACATAGGAAACCCATCAAATTTTGCTTCTCCT10.8928571428571428No Hit
GCAGGCAAAAAGAAGAGTCACCATTCTGGCATCAGGGAAAGGTTAGATAG10.8928571428571428No Hit
CTTTCCTATTTGAAGGCTATGCAAATAAAATAAAATCGTGTTGATGGCTT10.8928571428571428No Hit
GTCATGACAATACTTAAAAAAATGCTAGAGTATTTTAAACCAAATCTAAG10.8928571428571428No Hit
GCCTTGGAAAGCGTCGCGGTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGC10.8928571428571428No Hit
GCCCGCGACATTGGCATGGACCTGCACCGGGTCGCGCGCGACAAGCGAGC10.8928571428571428No Hit
GTTCAGCTCTAGAGCATGCTTAATATGTCATAGCGTATTGAAATGTTGTG10.8928571428571428No Hit
CAGAAATCCACTTTAAATATAAAGAATTAAATAGTTTAACTAAAAGGATA10.8928571428571428No Hit
GTACTCTGCGTTGATACCACTGCTTCCCCATGTACTCTGCGTTGATACCA10.8928571428571428Clontech Universal Primer Mix Long (96% over 27bp)
TTTTGATGCTGACCGCGCGCGGCGAAGATATCGATCGCGTAGTCGGTTTG10.8928571428571428No Hit
CGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCA10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAATCATAAGGGACAGGAGGGAGATCTGCATGCCAGGAGGCAACCGAAGG10.8928571428571428No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.8928571428571428Clontech Universal Primer Mix Long (96% over 26bp)
CTAAGCAATTAAATATTCAGGACTTTTATAAAGGAAGAGGTCAGTGCTTT10.8928571428571428No Hit
ATTCCATTCGATGATGTTTCCATTTGATTCCGTTCAATGATGATTGCATT10.8928571428571428No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.8928571428571428Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAATCTTATGTGATTAAAGTAGCTTGTTTTCATGGCACTTTTTAAGG10.8928571428571428No Hit
GCATAGTTCAGGCCGAGAACCGCTTTCAGGCTAAAAGTCATAAAAACAAA10.8928571428571428No Hit
GTGTATGCATCATTATCATCTTCACTCTCTTCTCCTATATGACAAGTGCA10.8928571428571428No Hit
GCGTGAGGCGGTGGTAGCGATCGGCGTGCACCGCACCGTCCTGCGCCGTC10.8928571428571428No Hit
CATCTGGACAGTTAGGTGGGCACGGCAGGCGTTTTCCTTCTTTTAACGTA10.8928571428571428No Hit
TCTTTAATGATGAAATGTCGCCAAGTCAGCTGAAGTCATTGGCAACAGCA10.8928571428571428No Hit
GCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACC10.8928571428571428No Hit
ATTCTGCTTTTCCTGGGATTGGCAGAAGCCTGTACTCCTCGTTAAGGTAA10.8928571428571428No Hit
GGTTGATTTGGTGTTCAGCACAAAGGGCCTCCACCAACTTGACATACATA10.8928571428571428No Hit
GTTTTGAAACCGTCTTTTTGAGGAATCTTCCAGTGGGCATTTTGAGGGCT10.8928571428571428No Hit
GAATAAAACGGACTCAATAGTAATGGATTGCAATGTAATTGATTCGATTT10.8928571428571428No Hit
GATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACG10.8928571428571428No Hit
CCGAAGACCCGCGCGCCGAACTCGTGCGCCGCCTGCTCGAGTACGAGCAG10.8928571428571428No Hit
TCCCCTAGGAGAACAGTGAGGCAGAAAAGGCAGAAGCCTTTGGTATGGGG10.8928571428571428No Hit
CTACAGGCGCCCGCCACCACGGCCAGCTAATTTTTGTGTTTTTAGTAGAG10.8928571428571428No Hit
CATATTAACTCATTTCATGCACTGCCATTTCCACTTTCAGATCAGCAAAC10.8928571428571428No Hit
CTCTCACTGTGTGTGTCTGTCTTCTGTCTTACTCTCCTTCTCTGCCTGTC10.8928571428571428No Hit
GAGGTCAGGAGTTTGACACCAGCCTGGCCAACAAGGCGAAACCCCATCTC10.8928571428571428No Hit
TGGATGTTGCGCGCAAATACCCCGAGTTCCCGATTCTGCTGGAAACCGCC10.8928571428571428No Hit
CACAAATACAGTGAGACGAGATGCCTGAGAACTTATCTGATCACCCTTCA10.8928571428571428No Hit
CACTAATTCTTTTTATACATTTTTTTTTTTTTACATTCCAGAAATAAGCG10.8928571428571428No Hit
ACAGTGACTTTTTGGGTGAGCCGTGTGTGTCTGTCTGTTGTGTGTGTGCC10.8928571428571428No Hit
GATCGCAATGGCGCGGGATCCTCCAGATTCGGTCGACTGGATGTGGATGT10.8928571428571428No Hit
GGTTGCAGTGAGCTGAGATCATGTCACCACACTCCAACCTGAGTGACAGA10.8928571428571428No Hit
AGTCAAAGAAGCAGTTTATGACCTTCAAGCATTTAGCAAACCTAATATCT10.8928571428571428No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)