FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107
Sequences flagged as poor quality0
Sequence length140
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGAAGCTGCCGATCCACATCGATTTCGACTGCCTCAAGAAAAATGCC32.803738317757009No Hit
CCATACTCCCCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGG32.803738317757009No Hit
GTCTGCAGGCTGTGCAATGGCGGGCTGTCCTGGCACACGCCCTGCTGTGC21.8691588785046727No Hit
AGCCGGCACCGCCTTCGATCTGGATCTCGGCGTTGGCGAAGAAGAAGGCC21.8691588785046727No Hit
GTTATTAGTCAACCACTCAGCTAACCAAACAGAAACTTCAGTGGCCACAC21.8691588785046727No Hit
GAATGCATGCACTTGAAACAAATAGATCTGACTAATGCAAGCTACTACTA21.8691588785046727No Hit
GAGTGAGACTCTGTCAAAAAAAAAAAAAAGAGGTAGACAGTAAATATTTT21.8691588785046727No Hit
CAGGTGATCTGCCCGGCTCAGCCTCCCAAAGTGCTGGGATTACAGGCGTG21.8691588785046727No Hit
AGTCGCCTTTCACTCTCCGTCACTTAATTTTGGACTGAACAGCACAGTAA21.8691588785046727No Hit
AAACTAGACAGAGTGATTCTCAGAAACTCCTTTGTGATGTCTGCGTTCAA21.8691588785046727No Hit
GTCGACACCTATTGTTGAACAGGCAACATCACACCAAGTCAGCTAAATAG21.8691588785046727No Hit
ATCTTCAACCCATCACCACGATCATTGCGGTCGCGCATCACGGTGGTGGT21.8691588785046727No Hit
CACAAATGCAGACCAGCCACTGGACCTTGCGACCAAGAAAAAGATCCACG21.8691588785046727No Hit
GGGCAGGGCCAAGCAGTCCAGGGCAGAACAGCTGACCTGTTGCTGCGAAG21.8691588785046727No Hit
TATAGTTTTTGTTCTTTATTTTGTTGACATGACATATCACATTAATTAAT21.8691588785046727No Hit
GGCGTGATCTTGGCTCACTGCAACCTCCGCCTCCCAAGTTCAAGTGAGTC21.8691588785046727No Hit
GCGGGATGCCGTGCTTGTGCCAGAGCGTCATGAAGCTCGACCACCAGCGC21.8691588785046727No Hit
TGCCCTGGATTTTTCCCTCAACCCCTTCCTCTCCGTTGTTTCCAAGCATC21.8691588785046727No Hit
CAGGGACATGACGATGACGTTGTCGAGAAGGCCATGGCGACGACCGGGGT10.9345794392523363No Hit
GCATCGAAGCCACCGCGGGCGCGGCGATCGTGCACCGTTGGGTGCCCGGG10.9345794392523363No Hit
GTCTGTCCTTGATGGGCATTTAGGTAGACTGGATAAAGAAAACTGTACCA10.9345794392523363No Hit
GTCCATGGCGGCCTCCGCAAAGAAACCAATCTGGGCCGCGGTTGCGGGAC10.9345794392523363No Hit
AGGATTTCAAGACCAGCCTGGCCAACGTGGTAAAATCCTGTCTCTACTAA10.9345794392523363No Hit
ATCCTAGATGGTAGCAGAAAATATAAAATAAGTCAGAAAAAATATAAAAT10.9345794392523363No Hit
GTAAAACTTTGAGGCAAAGGAGTGGAAAGGTCTCAGCAATATGTAAGGCT10.9345794392523363No Hit
GGCCTAAACTGGTGCAGGTCTTGGTAGGGCGCAACAACAAGCAGAATGAT10.9345794392523363No Hit
CCTCCCTCCTGTCCCTGCTGCTCTTTGCTGGGATGCAGATGTACAGCCGT10.9345794392523363No Hit
ATCTTAGAGATCTACTTCTTAGAGTAGAATGAAGAGATACTATCTGGAAC10.9345794392523363No Hit
GCCATGGTGCTTCCTGGATGCCACAGTGCTCTAAGAGCTTTATATGCTCA10.9345794392523363No Hit
ATATGTAGAAAGCTGAAATTGAATCCCTTCCTTACACCTTATACAAAAAT10.9345794392523363No Hit
GACTATGCTACAGTAGCAAACCAACATCTGAAAAATTCTATAAATATGCC10.9345794392523363No Hit
GGTGTATATGCCACCAAAACCTTTTGCCACCTTAAAAATTTCCTTCAAAG10.9345794392523363No Hit
GTTTGTTTGTTTCTTGGAGACAGAGTCTCACTCTGTCACCCAGGCTGGAG10.9345794392523363No Hit
TCAGGAAAGGTTGTTAACATCTTTGTTTCAAAGTTAAACTCCAAACTAAG10.9345794392523363No Hit
GCCATGTGCGGCCCCACGAGCCCCACGAATCCGATGATCCCGACGAAGGC10.9345794392523363No Hit
GTCTGCACCGATACGAAAAGCCGAGACCGAGCCGGCCTCGAACTCGTTGG10.9345794392523363No Hit
AGCAAGCTGCATGCACCTTTTCGCTGCTGTTTGTCAGCTTTTGGCGAGGC10.9345794392523363No Hit
ATGCTACATAGCACAGCCAAAAGTGAAAGTGTCAGCTTGAGAAATGAAGA10.9345794392523363No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC10.9345794392523363No Hit
GGTGAAAATATGAAAAATAAAAAAGAATGAGAAAGTGAAGAAGAAAAAAA10.9345794392523363No Hit
GAATAAATATTTAAAAAATATGAAGATGTGAAATCCATAGTGTTACTTTT10.9345794392523363No Hit
ACACAAGTGCAGCTGTCCCCAAGGGTACAGGACCCTACTGGTGGGTAGCG10.9345794392523363No Hit
ATTAGGAATAGAGAGACTAATTTTGATTGTTGGTTATGAAAGCCTTCTCT10.9345794392523363No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTACG10.9345794392523363Clontech Universal Primer Mix Long (95% over 22bp)
ATTTTAAGCAATTAATCTTCCTGTGGATGAATTAAACCCATTAGATGCTG10.9345794392523363No Hit
ATGTTGATTTGAGTTGAAAAGTATGGCAGTGATCCTCTGACAACAAGGTA10.9345794392523363No Hit
CGGCGTAAGTGAGATGTTCGATCACGTGGAGCGGCTCGGCCAGCCCTGCT10.9345794392523363No Hit
GAGTGAGACTCTGTCAAAAAAAAAAAAAAGAGGTAGAAAGTAAATATTTT10.9345794392523363No Hit
CCCTGGTCGTCCTTCGTCGCGTCCGGGTACTTGATGCGCTTGGAGATGTC10.9345794392523363No Hit
ACCCTCTGCTGAATTCGGTTCTCTTTTCTGCCTCCACGCTTAATGATTCC10.9345794392523363No Hit
GTGTATACTGCTGGGGTTATGGTTGCACCAAAATCTCACAAATCACTGCT10.9345794392523363No Hit
CTAAATAGTCTTTTGGTAAACAGAAATTCTGAATTTTAACGCAGTCCAAA10.9345794392523363No Hit
AGAACCAAGTGAAGGAAGTGTCTGAAGCTGTGCAGAAAGAGGGGATGACT10.9345794392523363No Hit
CTCCCCCTCCTTGGTTCAAGCAGTTCTCCCGCTTCAGCCTCCCAAGTAGC10.9345794392523363No Hit
GATCTGTTCCGCGCCGGCGTCAACGTGGTGCGCCTGAACTTCAGCCATGG10.9345794392523363No Hit
CAACTGCTTCACTCAAGCAATCTACCCACCTCGGCCTCCCAAAATGCTGG10.9345794392523363No Hit
GAAGGCACAAAAGACTCACTCACCTGAAGCTGAATCTATTTCAAACACTG10.9345794392523363No Hit
GCGATGGTCCAGTCACTGTAGTCCATTTGCCATGTTTCGTTGGGCTGATC10.9345794392523363No Hit
GTACAAGGACTAAAACTGGGACATTTGTGAGATTTATCAACAAGCTTTTC10.9345794392523363No Hit
TATTTGAGCAATACAGAGGATTAAGAGATCAAGATACTTCACTGCCTATT10.9345794392523363No Hit
AGATATGGCTGTGCTGGTGCTGGAAGTAATCACCAAACTTGATGGTCTTC10.9345794392523363No Hit
GTCATAAATAGCTCTTATTATTTTGAGATACATCCCATCAATACCTAGTT10.9345794392523363No Hit
GAAGGATGGGATGTGGTGAAAGCCTGGGTAGCAGTAGTTCTGCAGAGACA10.9345794392523363No Hit
CGATTGCAAAGGAATGGAATAGAATGGAATGGAATGGAATGGAATGGAAT10.9345794392523363No Hit
AGCCAGCTGCGTGACCTGCGTGTATTTCGTGCGCCTACAGCATTCTCTGG10.9345794392523363No Hit
GAACAATATACTCATTTAGGGGTTGAAGTTTGAAAGGAAATCAGATGATG10.9345794392523363No Hit
GATTTTAACGTTTCTTTTCTAACTTGAATGTTTTTTAGCTCTTTTTCTTG10.9345794392523363No Hit
GAGAAGCCAGAGAAAGATGGGGGCCCTGAAGCTGTTTCCACATGGTATGT10.9345794392523363No Hit
CCATTGCCTGGGCGATGCCGCGCGCCGCGAAGTCGCGCACCCCCTGCACC10.9345794392523363No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAATTAAA10.9345794392523363Clontech Universal Primer Mix Long (95% over 22bp)
ATATTAGACAGATCAATGAGACAGACCCGCCCCCCGGCGCGGCCGGCCGG10.9345794392523363No Hit
AGTGGGTGCCCTACGCGCAGATCAACGACACGCTCAAGCGCGCGGTGATC10.9345794392523363No Hit
GTGTGATGTATGCGACACCATGCCAGGCGTCTTGGCAGCAAGACAAGCCA10.9345794392523363No Hit
CCTGTGACAAGGAACGTCCACAAATAATGCACAAATATTTGGTGCTGACA10.9345794392523363No Hit
ATGCTGGACAATGGCCCTCCATACCCTGACTGGAACATGGTTGGACAATG10.9345794392523363No Hit
GTCTTGGAGCGTTTCCCCAAAGTTTTCTTTTGGTAGTTTCATAGTTTTGG10.9345794392523363No Hit
CGATGATTCCACTCGATTCCATTAGATGATTCCATTGGAGTCCATTTGAT10.9345794392523363No Hit
CCCATCAGCTGCGCGCGGCCTACCACGCGCTGCAAGCCCAAAGCCCGCAA10.9345794392523363No Hit
CCTCAATGGACGGCTACGCCGCCTTCGGTGCCTCGATTACCGAAAACGGT10.9345794392523363No Hit
CTGCTGATGGCTGAGATATTTCTGACTCTGGAATTTATCCTTGAGTATAG10.9345794392523363No Hit
ACTTTATAATTTGTCAAAACTTCCATAGCTGCTTGTGGTTCTTAACTTAG10.9345794392523363No Hit
TTATTGGTCTATTCAGGTTTTCAGTTTCTTCCTTCTTCAATCTTAGGAGG10.9345794392523363No Hit
ATGGAATGTTATTCATACATAAAATAGGGTGAAATCCTGTCATTTGCAGC10.9345794392523363No Hit
GTGACTTGAATGAAAACATCACAAAGCAGTTTCTGAGAATGCTTCCGTCT10.9345794392523363No Hit
GTTTATACGAATTCCCGCTTCCAACGAAATCCTCAAAGCAATCCAAATAT10.9345794392523363No Hit
GTCTTGGGATGCACCTGTAAGGAGCAAGAATCCAACACTCCCCAATGGCA10.9345794392523363No Hit
CAGCAGCAGGCACAGCAGCCGCAACCTTCGGAAGCCGCCGAATTCATCCT10.9345794392523363No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)