FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA992.308768656716418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT641.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC511.189365671641791No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG340.792910447761194Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG310.7229477611940298Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT280.6529850746268656No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT250.5830223880597015No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT180.4197761194029851Clontech Universal Primer Mix Long (96% over 26bp)
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG170.396455223880597No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC160.3731343283582089Clontech Universal Primer Mix Long (96% over 26bp)
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT160.3731343283582089No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC150.3498134328358209Clontech SMART CDS Primer II A (96% over 26bp)
GCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGC140.3264925373134328No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC140.3264925373134328Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC140.3264925373134328No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG130.3031716417910448No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT130.3031716417910448No Hit
GCCCCACACCGCGCCGGAGGACGGGTCGTTGAGCCCCGAAACCACCGTCC130.3031716417910448No Hit
GTCTACGTGAGCCTCGTGGCGGCGGCGCCCTACGTGCTGCGGATCGGCCT130.3031716417910448No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG120.2798507462686567Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC120.2798507462686567Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG120.2798507462686567Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120.2798507462686567Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC120.2798507462686567Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG110.25652985074626866No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT110.25652985074626866No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC110.25652985074626866No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT100.23320895522388058Clontech Universal Primer Mix Long (100% over 23bp)
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGC100.23320895522388058No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG100.23320895522388058No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT100.23320895522388058No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC100.23320895522388058No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA100.23320895522388058No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT90.20988805970149255No Hit
GCCTAGTACCGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC90.20988805970149255No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG80.18656716417910446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAG80.18656716417910446No Hit
AGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAG80.18656716417910446No Hit
CGGCAAGAGCGATTGCCGAGCTTAGAGCGTATCTTCCGGGATCGGGCAAA80.18656716417910446No Hit
GTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTT80.18656716417910446No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG80.18656716417910446No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT80.18656716417910446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTACGTGAGCCT80.18656716417910446No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC80.18656716417910446No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG80.18656716417910446Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80.18656716417910446Clontech Universal Primer Mix Long (96% over 26bp)
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG70.1632462686567164No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG70.1632462686567164No Hit
ACCTACAACTTGCGCCAATTATCGAGCTGGTAGACGACCAGCGCTGACGA70.1632462686567164No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT70.1632462686567164No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA70.1632462686567164No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC70.1632462686567164No Hit
GTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTGAGCCC70.1632462686567164No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC70.1632462686567164No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT70.1632462686567164No Hit
CGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTC70.1632462686567164No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC70.1632462686567164No Hit
CTACGTGACCGACTCCCATCACCCGCGCGTGCTGAAGCTCGCCCCAGGTG70.1632462686567164No Hit
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT70.1632462686567164Clontech SMART CDS Primer II A (96% over 26bp)
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT70.1632462686567164No Hit
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG70.1632462686567164No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT70.1632462686567164Clontech Universal Primer Mix Long (96% over 26bp)
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC70.1632462686567164No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG70.1632462686567164No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC60.13992537313432835No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA60.13992537313432835No Hit
GTACTAGGCCCTTATAGGGGACGAACCCGGGGCTGCGCGTGACGAACCAC60.13992537313432835No Hit
GGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGA60.13992537313432835No Hit
CCGTAGGGGCCCTCGCCGAAGGCATTGCTCTCGAACAGCACGAGCCGCCC60.13992537313432835No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
GAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA60.13992537313432835No Hit
GTGTTAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTCCCGATGC60.13992537313432835No Hit
CACTAGAGCGACACCAACATCGTTACGCTTACACACCGGACGCTTGGATC60.13992537313432835No Hit
GAATATCGGTTTGAGGCCCCGAGCTGGAGGGTTCTTATCTGCCTAGTACG60.13992537313432835No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACCG60.13992537313432835No Hit
GTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGT60.13992537313432835No Hit
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG60.13992537313432835No Hit
GTTTTGCTCCTTGGATTCTGAGCCCGGGCATCGCCCCTCGTTTATGAACT60.13992537313432835No Hit
ACCTTATACAGAATTATCAATCAAGCTCCCCGAGGAGCGGACTTGTAAGG60.13992537313432835No Hit
GTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGG60.13992537313432835No Hit
GCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCG60.13992537313432835No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT60.13992537313432835No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT60.13992537313432835No Hit
GTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAG60.13992537313432835No Hit
GCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTC60.13992537313432835No Hit
ACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
AGTGTATTTAGGACGCACGCAGCCCTATTACGAGGGTTGTAGTCGTGTGG50.11660447761194029No Hit
CATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGC50.11660447761194029No Hit
GAAGTAGACGCGGTCGCCCCACACCGCGCCGGAGGACGGGTCGTTGAGCC50.11660447761194029No Hit
GTGCAGCACCTACCGCGGCAGCGCGCGCGACTGGATGTCGAAGGGCACGC50.11660447761194029No Hit
TGTATAGACAGTGCGCGTATCATATAGGATGCGGCGATCTCATACAGCGT50.11660447761194029No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA50.11660447761194029No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC50.11660447761194029No Hit
GGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCT50.11660447761194029No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG50.11660447761194029No Hit
AGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGC50.11660447761194029No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50.11660447761194029Clontech SMART CDS Primer II A (96% over 26bp)
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG50.11660447761194029No Hit
GGATGTCGAAGGGCACGCCGGTCGGCACCGGCCCGTCGCCCTCCTTGCGC50.11660447761194029No Hit
GCATTGGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCG50.11660447761194029No Hit
CCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGC50.11660447761194029No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC50.11660447761194029Clontech Universal Primer Mix Long (96% over 25bp)
GCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGA50.11660447761194029No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT50.11660447761194029No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC50.11660447761194029No Hit
CTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGT50.11660447761194029Clontech SMART CDS Primer II A (96% over 26bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA50.11660447761194029No Hit
GCCAATAGATGCCTAGTACGCTGACGGCATTCAGAGTACGCTGATCGGCT50.11660447761194029No Hit
TCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGG50.11660447761194029No Hit
CTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTACG50.11660447761194029No Hit
TCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGAAT50.11660447761194029No Hit
TCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTT50.11660447761194029No Hit
ACGCACACTAGACTAATTGATCGGACCGATGACAGTTCACAGAGCATCCT50.11660447761194029No Hit
TTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACG50.11660447761194029No Hit
TCACAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTA50.11660447761194029No Hit
GGTCCCTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGT50.11660447761194029No Hit
GAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGAC50.11660447761194029No Hit
ACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGA50.11660447761194029No Hit
CGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGC50.11660447761194029No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG50.11660447761194029No Hit
GTGTGGTGCCCCCGACCTACAACTTGCGCCAATTATCGAGCTGGTAGACG50.11660447761194029No Hit
TATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGA50.11660447761194029No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC50.11660447761194029No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
CAGAACAACCGCCGCATACGGCCGATTGTCGCAGCCCGGGTCGATTATAA50.11660447761194029No Hit
GTCTTCCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGA50.11660447761194029No Hit
CCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGC50.11660447761194029No Hit
GTACATGGGTCCCGCCCCACCGCAATTTCCATGCGCATATGAGTGCTATA50.11660447761194029No Hit

[WARN]Adapter Content

Adapter graph