FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4288
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT912.122201492537313Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA691.6091417910447763Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC481.1194029850746268No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG320.7462686567164178Clontech Universal Primer Mix Long (96% over 26bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT280.6529850746268656No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC240.5597014925373134Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC240.5597014925373134Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT240.5597014925373134Clontech Universal Primer Mix Long (96% over 26bp)
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG230.5363805970149254No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC220.5130597014925373Clontech SMART CDS Primer II A (96% over 26bp)
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC180.4197761194029851No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC170.396455223880597Clontech Universal Primer Mix Long (96% over 26bp)
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG150.3498134328358209No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG150.3498134328358209No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA150.3498134328358209No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG140.3264925373134328Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC140.3264925373134328No Hit
CGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTC130.3031716417910448No Hit
CGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGG130.3031716417910448No Hit
GTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGG120.2798507462686567No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC120.2798507462686567No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG120.2798507462686567No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA120.2798507462686567No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT110.25652985074626866No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG110.25652985074626866Clontech Universal Primer Mix Long (96% over 26bp)
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC100.23320895522388058No Hit
GTATAAGGATGCTCTGTGAACTGTCATCGGTCCGATCAATTAGTCTAGTG100.23320895522388058No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC100.23320895522388058Clontech Universal Primer Mix Long (96% over 25bp)
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG100.23320895522388058No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT100.23320895522388058No Hit
GCCTAGTACCGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC100.23320895522388058No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
CAACAGGAACATCCTCGTGTTAGATATTGAGGCTGCTTCGTGTCGGCACG100.23320895522388058No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC100.23320895522388058Clontech Universal Primer Mix Long (96% over 26bp)
GTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCG90.20988805970149255No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT90.20988805970149255No Hit
GCCCTATTACGAGGGTTGTAGTCGTGTGGCACCAGCGCGGCTACTCCGAT90.20988805970149255No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG90.20988805970149255No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA90.20988805970149255No Hit
ATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATATTGAGGCTGC80.18656716417910446No Hit
GTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACT80.18656716417910446No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG80.18656716417910446Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC80.18656716417910446No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT80.18656716417910446No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA80.18656716417910446No Hit
CTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTACG80.18656716417910446No Hit
CATATAGGCTGCACACCTTCTGAATGAGTCACACAGCTATCGACAGAGTC80.18656716417910446No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA80.18656716417910446No Hit
AGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGC80.18656716417910446No Hit
CCGATGCAGTGTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCC80.18656716417910446No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT70.1632462686567164Clontech Universal Primer Mix Long (100% over 23bp)
CTATAAGGGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGCAGATAAGA70.1632462686567164No Hit
GTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGCTGGTC70.1632462686567164No Hit
ATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAG70.1632462686567164No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG70.1632462686567164No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC70.1632462686567164Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC70.1632462686567164Clontech Universal Primer Mix Long (96% over 26bp)
GCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTG70.1632462686567164No Hit
GGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCC70.1632462686567164No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA70.1632462686567164Clontech Universal Primer Mix Long (96% over 26bp)
ACTCGAGCTAATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATA70.1632462686567164No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG70.1632462686567164No Hit
GTACAGAACTGAGTGTCATGTGTCCAAAGTTAAGCTCTGCACCTGTTACA70.1632462686567164No Hit
GCTTAGAGCGTATCTTCCGGGATCGGGCAAAGGGGCAACTCGAGCTAATC70.1632462686567164No Hit
AGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACC70.1632462686567164No Hit
GTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGAT70.1632462686567164No Hit
CCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGA70.1632462686567164No Hit
GCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGC70.1632462686567164No Hit
TCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGAC70.1632462686567164No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG60.13992537313432835No Hit
CTTCACCACCGTGCCATCGACGCCGAGCGCGCTCAAGCGCTATGACCTGG60.13992537313432835No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG60.13992537313432835No Hit
GAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTTAGATA60.13992537313432835No Hit
CTATAAGCGCTGTCACGGATGTATAAATCGCTCGAGAATCACACACAGTC60.13992537313432835No Hit
GCCATAGCCCAGATGCGAGGTGGAAAAGTCACACTAGAGCGACACCAACA60.13992537313432835No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC60.13992537313432835Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTATGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTCGTGTT60.13992537313432835No Hit
GTTGTAGGTCGGGGGCACCACACCACGCTCGCTTGGCAGTAACGCGAGCA60.13992537313432835No Hit
GAGTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGG60.13992537313432835No Hit
TTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCT60.13992537313432835No Hit
GATGTGTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAG60.13992537313432835No Hit
CATGTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCAT60.13992537313432835No Hit
CGTTACTGCCAAGCGAGCGTGGTGTGGTGCCCCCGACCTACAACTTGCGC60.13992537313432835No Hit
CGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCTCA60.13992537313432835No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT60.13992537313432835No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT60.13992537313432835No Hit
TCGGAAGACACTTCGTGCCGACACGAAGCAGCCTCAATATCTAACACGAG60.13992537313432835No Hit
ATCCCGCACTGGCCGGCGCGGGTCCCTACAAGGGCCTGAACGGCATCGCC60.13992537313432835No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC60.13992537313432835No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT60.13992537313432835No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA60.13992537313432835No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA60.13992537313432835Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTCCCGCCCCACCGCAATTTCCATGCGCATATGAGTGCTATA60.13992537313432835No Hit
CCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGC60.13992537313432835No Hit
GTCTACGTGAGCCTCGTGGCGGCGGCGCCCTACGTGCTGCGGATCGGCCT60.13992537313432835No Hit
GTACTAGGCCCTTATAGGGGACGAACCCGGGGCTGCGCGTGACGAACCAC50.11660447761194029No Hit
CCGTAGGGGCCCTCGCCGAAGGCATTGCTCTCGAACAGCACGAGCCGCCC50.11660447761194029No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACAAGGGCCTGAACGGCATCGCCATCGACGGCGGGCGCGATGTCTA50.11660447761194029No Hit
GTCAAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCC50.11660447761194029No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA50.11660447761194029Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGATGTATGCCCCATAGTGCTCAGTGGAGTCTCTCTTAGCATAGTG50.11660447761194029No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC50.11660447761194029No Hit
GCCCTACGTGCTGCGGATCGGCCTCGGCGCGGACGGCAAGGCCGGCGAGG50.11660447761194029No Hit
GATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTT50.11660447761194029No Hit
CCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATAGTGTA50.11660447761194029No Hit
GTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTAT50.11660447761194029No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG50.11660447761194029No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT50.11660447761194029No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50.11660447761194029Clontech Universal Primer Mix Long (96% over 25bp)
GGATGACGATGCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGC50.11660447761194029No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT50.11660447761194029No Hit
AACCTGCGCCGTCTTGTTAACTTGTCATATCGCGCACGTAGTAGCCTAGA50.11660447761194029No Hit
GTGTAGTGCGCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGG50.11660447761194029No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAA50.11660447761194029No Hit
AATCTCCCCAGCGGCTAGCATGTGTAGTGCGCCATATCATATCCAAGATA50.11660447761194029No Hit
CGCTCTTGCCGTGCGAGATGCTAGCAAAAAGGTGTACTTCTCAGCGGAGC50.11660447761194029No Hit
GCCTAGTACGCGCGGGGCTCGAAAGGTACTAGGCCCTTATAGGGGACGAA50.11660447761194029No Hit
GCCCGGGTCGATTATAACAACGGTGCAATCTCAGCTAAACCGACGCAGTT50.11660447761194029No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.11660447761194029Clontech Universal Primer Mix Long (96% over 26bp)
ATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCA50.11660447761194029No Hit
CTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTC50.11660447761194029No Hit
GTACATGGGCATCGCGTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAG50.11660447761194029No Hit
CGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGG50.11660447761194029No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)