FastQCFastQC Report
Tue 10 May 2022
EGAF00003645466

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645466
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413401
Sequences flagged as poor quality0
Sequence length140
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT7990.19327481065599744No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT6440.15578094876403298No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5620.1359454863437679No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5180.1253020674841135No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC4920.11901277452159043No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT4880.11804519098889456No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA4790.11586812804032888No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4770.11538433627398094No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4680.11320727332541528No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4630.11199779390954545No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC4620.11175589802637148No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT4570.11054641861050166No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC4450.10764366801241408No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC4380.10595039683019634No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG4240.10256385446576084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)