FastQCFastQC Report
Tue 10 May 2022
EGAF00003645711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences920984
Sequences flagged as poor quality0
Sequence length140
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42680.4634173883585383No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT42520.46168011604979026No Hit
GCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTC30940.3359450327041512No Hit
CTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCT27060.29381617921701136No Hit
GTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGGCCTGGATCCG24960.2710144801646934No Hit
CTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCC24220.26297959573673374No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCT23740.2577677788104896No Hit
GTCTGGGACAGACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATT23070.25049295101760727No Hit
CATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTTCAGTG21230.23051431946700487No Hit
GTCATACAGTTGACAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGG20800.22584540013724452No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG20240.2197649470566264No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT19000.20630108666382913No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18840.2045638143550811No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC18770.2038037577200038No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17740.19262006723243835No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC17550.19055705636580006No Hit
CCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACCAG17190.18664819367111696No Hit
GTCTATTATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGG16000.17372723087480346No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15640.1698183681801204No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA14870.16145774519427047No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA14730.15993763192411595No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14610.15863467769255493No Hit
AGTTACTACTGGGCCTGGATCCGCCAGCCCCCAGGGAAGGGGCTTGAGTG14560.15809178009607117No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14490.1573317234609939No Hit
GTATTAACAAGAATTACTTAGCCTGGTACCAGCAGAGACCTGGCCAGGCT14060.15266280413123354No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGT13660.14831962335936347No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC13600.14766814624358296No Hit
CATCTGCACTGTGTCTGCTTACTCCATCAGTCGGACCAGTTACTACTGGG13480.14636519201202192No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13450.14603945345413166No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13230.14365070402960312No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12790.13887320518054602No Hit
GTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGGACCAAGCTGG12740.13833030758406226No Hit
CTCCTGTAGGCTCAGTCAGAGTATTAACAAGAATTACTTAGCCTGGTACC12540.13615871719812722No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12540.13615871719812722No Hit
ACCTTCTACAGTCCGTCCCTCAGGGGTCGCGTCACCATATCCGTGGACAC12240.13290133161922466No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12220.13268417258063117No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA11950.12975252555961883No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT11760.12768951469298054No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11600.1259522423842325No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC11480.1246492881526715No Hit
GTCTTTGTCTCCAGGGGAAAGAGCCTCCCTCTCCTGTAGGCTCAGTCAGA11350.12323775440181371No Hit
CAATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGT11030.11976320978431763No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA10950.11889457362994361No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10920.11856883507205336No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC10690.11607150612822807No Hit
ATTGTCAACTGTATGACAGGTCACCTCCGATGTACAGCTTTGGCCAGGGG10670.11585434708963457No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAGCTG10600.11509429045455731No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA10500.11400849526158978No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG10480.11379133622299628No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10450.11346559766510601No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT10440.11335701814580927No Hit
GACTTCACTCTCACCATCACCAGACTGGAGCCTGAAGATTTTGCAGTCTA10430.11324843862651252No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG10390.1128141205493255No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10380.11270554103002875No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT10280.11161974583706123No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC10210.11085968920198395No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC10160.1103167916055002No Hit
CTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCAGACAGGTT10120.1098824735283132No Hit
ATAATAGACTGCAAAATCTTCAGGCTCCAGTCTGGTGATGGTGAGAGTGA10020.10879667833534566No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA10010.1086880988160489No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9920.10771088314237816No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9670.10499639515995934No Hit
CTCATAAGCAGCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTCTCTGCT9650.10477923612136585No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9600.10423633852488208No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9510.1032591228512113No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG9450.1026076457354308No Hit
CTCCCAGGCTCCTCATCTATGCTGCTTATGAGAGGGCCACTGGCATCCCA9400.10206474813894703No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9380.10184758910035353No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG9340.10141327102316652No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9320.10119611198457301No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9270.10065321438808925No Hit
GCAGAGACCTGGCCAGGCTCCCAGGCTCCTCATCTATGCTGCTTATGAGA9240.10032747583019899No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9230.10021889631090225No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)