FastQCFastQC Report
Tue 10 May 2022
EGAF00003645820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377682
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT8800.23300024888662949No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA7920.20970022399796656No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT6590.1744854136548737No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6000.15886380605906555No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC5540.1466842475945372No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT5420.1435069714733559No Hit
CCTTACACTATTCCTCATCACCCAACTAAAAATATTAAACACAAACTACC5320.14085924137237146No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT5210.1379467382612886No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG5170.13688764622089483No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA5120.1355637811704026No Hit
CTCTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTC5090.1347694621401073No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC5020.13291605106941817No Hit
GTGTATGAGTTGGTCGTAGCGGAATCGGGGGTATGCTGTTCGAATTCATA4940.13079786698863063No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4850.12841490989774468No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA4810.1273558178573509No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA4780.12656149882705556No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT4710.12470808775636648No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT4690.12417854173616957No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC4660.12338422270587425No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT4610.12206035765538203No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT4540.12020694658469294No Hit
CTATTGATCCCCACCTCCAAATATCTCATCAACAACCGACTAATCACCAC4480.1186183085241023No Hit
CCCCATACCCAACCCCCTGGTCAACCTCAACCTAGGCCTCCTATTTATTC4360.11544103240292097No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4330.11464671337262564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGCAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA4310.11411716735242876No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT4300.11385239434233033No Hit
ACCATACACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTT4260.11279330230193656No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4230.11199898327164122No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC4210.11146943725144434No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA4200.1112046642413459No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC3940.10432056597878639No Hit
CATTTACACCAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCC3920.10379101995858951No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT3920.10379101995858951No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT3910.10352624694849105No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG3880.10273192791819573No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG3850.1019376088879004No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC3830.10140806286770353No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT3820.10114328985760508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)