FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003645904

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003645904
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2766
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT160.5784526391901663Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160.5784526391901663No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG150.5422993492407809Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA140.5061460592913956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.43383947939262474No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA100.3615328994938539No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70.2530730296456978Clontech Universal Primer Mix Long (96% over 26bp)
GCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAG60.21691973969631237No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.21691973969631237Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG60.21691973969631237Clontech Universal Primer Mix Long (96% over 26bp)
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA60.21691973969631237No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG60.21691973969631237Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50.18076644974692696Clontech Universal Primer Mix Long (100% over 23bp)
CCATTGGCGTGAAGGTAGCGGATGATTCAGCCATAATTTACGTCTCGAGT50.18076644974692696No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG50.18076644974692696No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG50.18076644974692696No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG50.18076644974692696No Hit
GGGCTATTGGTTGAATGAGTAGGCTGATGGTTTCGATAATAACTAGTATG40.14461315979754158No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA40.14461315979754158No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG40.14461315979754158No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA40.14461315979754158No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT40.14461315979754158No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC40.14461315979754158No Hit
CTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTC40.14461315979754158No Hit
CATTACTGCAGGCCACCTACTCATGCACCTAATTGGAAGCGCCACCCTAG40.14461315979754158No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC40.14461315979754158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA40.14461315979754158No Hit
TATCCGGAATGCCCCGACGTTACTCGGACTACCCCGATGCATACACCACA40.14461315979754158No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC40.14461315979754158No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.14461315979754158Clontech SMART CDS Primer II A (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.14461315979754158Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA40.14461315979754158No Hit
ATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTGTATGAGTTGG40.14461315979754158No Hit
ACGCAGAGTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAG40.14461315979754158No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.14461315979754158Clontech Universal Primer Mix Long (96% over 26bp)
GTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGACGTCTAAACCA40.14461315979754158No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.14461315979754158Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGCTGTATTTGCCATCATAGGAGGCTTCATTCACTGATTTCCCCTATTC40.14461315979754158No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA40.14461315979754158Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.14461315979754158No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA40.14461315979754158No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG40.14461315979754158No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT40.14461315979754158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.14461315979754158No Hit
CTCCTTGGCGCCTGCCTGATCCTCCAAATCACCACAGGACTATTCCTAGC30.10845986984815618No Hit
GTGTAGAGGGAAGGTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATT30.10845986984815618No Hit
CATATAAGTAATGCTAGGGTGAGTGGTAGGAAGTTTTTTCATAGGAGGTG30.10845986984815618No Hit
GTTTTTAGGATATTGTGAGTAATATAGATTGGCGCTAGTAGATCACAGAA30.10845986984815618No Hit
GTATCAACGCAGAGTACATGGGTGCCCTCAACAAACCAGTTCCTGTAGTT30.10845986984815618No Hit
GTGCTATAGGGTAAATACGGGTCCTATTTCAAAGATTTTTAGGGGAATTA30.10845986984815618No Hit
ACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAA30.10845986984815618No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT30.10845986984815618Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.10845986984815618Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.10845986984815618Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGATGTAGAGGGTGATGGCAGATGTGGCGGGTTTTAGGGGCTCTTTGGT30.10845986984815618No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.10845986984815618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATATCATTCTGAGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCC30.10845986984815618No Hit
CATTTGAAGAATCAATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCC30.10845986984815618No Hit
GTCTAATAGAAAACAACCGAAACCAAATAATTCAAGCACTGCTTATTACA30.10845986984815618No Hit
GTACATGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG30.10845986984815618No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT30.10845986984815618No Hit
GGTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAA30.10845986984815618No Hit
ATTAAACACAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAA30.10845986984815618No Hit
TGCATACACCACATGAAACATCCTATCATCTGTAGGCTCATTCATTTCTC30.10845986984815618No Hit
CTGACATCCGGCCTGCTTCTTCTCACATGACAAAAACTAGCCCCCATCTC30.10845986984815618No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAA30.10845986984815618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG30.10845986984815618No Hit
GAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA30.10845986984815618No Hit
TAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTG30.10845986984815618No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA30.10845986984815618No Hit
GGTTAGAATGAGGAGGTCTGCGGCTAGGAGTCAATAAAGTGATTGGCTTA30.10845986984815618No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC30.10845986984815618No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAG30.10845986984815618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTAGGTG30.10845986984815618No Hit
GATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCATTTGG30.10845986984815618No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG30.10845986984815618No Hit
CAAACTACCACCTACCTCCCTCACCAAAGCCCATAAAAATAAAAAATTAT30.10845986984815618No Hit
GTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGTCATTGTTGGG30.10845986984815618No Hit
CCCCGGGACCTCTTGCCCCAATTCCTCCGCCTTGCCGGGAAAGGTTGATC30.10845986984815618No Hit
ATTGAGGAGTATGCTAAGATTTTGCGTAGCTGGGTTTGGTTTAATCCACC30.10845986984815618No Hit
GGTCTGCGGGTTATAGCTTTTCAGTCTCGACGGGCTAGCACACATCTGGT30.10845986984815618No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC30.10845986984815618No Hit
CGCTGGACCCGATGACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTT30.10845986984815618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT30.10845986984815618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG30.10845986984815618No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT30.10845986984815618Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCATGAGCTGTCCCCACATTAGGCTTAAAAACAGATGCAATTCCCGGAC30.10845986984815618No Hit
CCGTAGTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACG30.10845986984815618No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG30.10845986984815618No Hit
TTGTTAATGTGGTGGGTGAGTGAGCCCCATTGTGTTGTGGTAAATATGTA30.10845986984815618No Hit
GTGTGGTGGTAGGTACCCGTAGTTCCAGCTACTCGGGAGGCTGAGGCATG30.10845986984815618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA30.10845986984815618No Hit
TCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA30.10845986984815618No Hit
GTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTC30.10845986984815618No Hit
CACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTA30.10845986984815618No Hit
CTTTAGTGTTGTGTATGGTTATCATTTGTTTTGAGGTTAGTTTGATTAGT30.10845986984815618No Hit
TATCAACGCAGAGTACATGGGAGAGTACTTCGAGTCTCCCTTCACCATTT30.10845986984815618No Hit
CGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCT30.10845986984815618No Hit
CCGCTACATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTG30.10845986984815618No Hit
CTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCCATGCATT30.10845986984815618No Hit
GGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10845986984815618No Hit
GAATTAATTCTAGGACGATGGGCATGAAACTGTGGTTTGCTCCACAGATT30.10845986984815618No Hit
GAGTACATGGGGGAGTGATTTCTGCTTACTGGGAGGAGCACCTGACTCAT30.10845986984815618No Hit
CTTATACTAGTATCCTTAATCATTTTTATTGCCACAACTAACCTCCTCGG30.10845986984815618No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT30.10845986984815618No Hit
GTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCCCAACCGAAATTT30.10845986984815618No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA30.10845986984815618No Hit
ATTTGGAGGTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCG30.10845986984815618No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)