FastQCFastQC Report
Tue 10 May 2022
EGAF00003646029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences607428
Sequences flagged as poor quality0
Sequence length140
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT30880.5083730088175059No Hit
GTACATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGG23780.3914867276450872No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC23170.38144438517816104No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC21270.3501649578221616No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG20810.3425920438307091No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT19450.32020255898641486No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA16200.2666982753511527No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA16000.2634057040505212No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG15070.24809524750258466No Hit
GTACATGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGG15010.24710747611239522No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG14990.24677821898233207No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG14540.23936993355591119No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT14310.2355834765601849No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA13850.22801056256873242No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT13810.22735204830860614No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGCAGAGAGCATTCCATCTGG13570.22340096274784832No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG13510.22241319135765883No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT13290.21879136292696416No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG13280.2186267343619326No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC13110.21582804875639583No Hit
GTACATGGGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTG12930.21286473458582747No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT12920.21270010602079586No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT12890.21220622032570116No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT11900.19590799238757514No Hit
GAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG11670.19212153539184892No Hit
GTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGC11540.18998136404643842No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG11370.18718267844090164No Hit
GGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCA11070.18224382148995438No Hit
GGCCTGCAGAGAGCATTCCATCTGGAAGTTGTGGACTTTTCTAGTGAGAG10990.18092679296970177No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG10840.1784573644942281No Hit
GGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAG10720.1764818217138492No Hit
GGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCA10590.1743416503684387No Hit
TCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCA10140.16693336494201783No Hit
GCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGC10140.16693336494201783No Hit
AGATGGATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTAC10130.16676873637698625No Hit
GCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCT10060.1656163364217652No Hit
GCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCC9710.15985433664566007No Hit
GTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCACTCCCA9680.15936045095056534No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC9490.1562325082149654No Hit
GAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTC8930.14701330857319714No Hit
TTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGTCGTGGTGATTGTGGGA8330.1371355946713026No Hit
ACTCCGCAGCTCCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCAC8250.13581856615105No Hit
GTGTGGGCAGAAGCAGCTTCTTGCCCGTCTGCAAAAGCTGCAAAAGACCC8110.13351376624060793No Hit
GCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCACAA7900.13005656637494484No Hit
AGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCC7890.12989193780991326No Hit
CCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGC7750.1275871378994712No Hit
GTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAGGA7690.12659936650928177No Hit
TCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCAT7580.12478845229393443No Hit
GCCTGGCCCTGAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGC7570.12462382372890285No Hit
CCTACAAGCCAGCCCCTGGCACCTGCTGCTACATCATGAAGATAGCTCCA7490.12330679520865026No Hit
CCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGT7470.12297753807858709No Hit
GGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCAT7470.12297753807858709No Hit
GACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCA7390.12166050955833448No Hit
GACCCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGT7340.1208373667331766No Hit
GTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGGGGCCGATT7320.12050810960311346No Hit
ATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTGCCCAC7310.12034348103808189No Hit
GCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGCAGCTCCCCGG7060.11622776691229247No Hit
CCCCGGGGCCGATTTGGCATTCCCTGCTGCCCAGTGCACCTGAAACGCCT7050.1160631383472609No Hit
ATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAG6990.11507536695707146No Hit
CATGGGAGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAG6970.11474610982700831No Hit
GTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATT6880.11326445274172413No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA6820.11227668135153468No Hit
GTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGAT6750.11112428139631364No Hit
CGTCTAAGCTGGGCCAGGCAGAGGGGCGAGATGCAGGCTCAGCACCCTCC6750.11112428139631364No Hit
GAGTGAGCACCTGGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCC6580.10832559579077684No Hit
AGCCAGAAACACACGGAGATGGTTCTGGAGATGAGCATTGGGGCGCCGGA6560.10799633866071369No Hit
GCAAAAGACCCTTTGCCCGGGGCGTTTCCTTTCCCGTTGGGGCTTCCACT6560.10799633866071369No Hit
GATTGTGGGAGCCCTGCTCATGGGTCTCCACATGAGCCAGAAACACACGG6500.10700856727052424No Hit
ACCCTGCTCACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCAC6450.10618542444536636No Hit
GATGTGGGCAGCAAAGAGGTCCTGATGGAGAGCCCGCCGGACTACTCCGC6360.10470376736008219No Hit
GGGGAGAGGAGAGCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAG6300.10371599596989273No Hit
CCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGTGT6130.10091731036435594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)