FastQCFastQC Report
Tue 10 May 2022
EGAF00003646070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375585
Sequences flagged as poor quality0
Sequence length140
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT11160.29713646711130637No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC9200.24495120944659662No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC9190.2446849581319808No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC9070.2414899423565904No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT9060.24122369104197453No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC8300.2209885911311687No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG8050.21433230826577204No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC7890.2100722872319182No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC7600.2023509991080581No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC7290.19409720835496624No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC7090.18877218206264892No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6910.18397965839956334No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA6480.1725308518710811No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC6480.1725308518710811No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT6440.17146584661261766No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG6310.16800457952261139No Hit
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT6270.16693957426414793No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT6210.16534206637645274No Hit
TCACTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC6110.16267955323029407No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC5950.15841953219644023No Hit
GCCTACAGATGATAGGATGTTTCATGTGGTGTATGCATCGGGGTAGTCCG5850.15575701905028155No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG5800.15442576247720224No Hit
CTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAG5790.15415951116258636No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA5700.15176324933104357No Hit
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG5590.14883448487026907No Hit
CTCCTACTCCTGCTCGCATCTGCTATAGTGGAGGCCGGAGCAGGAACAGG5520.146970725667958No Hit
TAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTACGATGTCTAG5500.14643822303872625No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT5490.1461719717241104No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT5390.14350945857795175No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC5250.13978194017332962No Hit
ACCATAATCATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGC5200.13845068360025026No Hit
GTACTACGTTGTAGCTCACTTCCACTATGTCCTATCAATAGGAGCTGTAT5180.13791818097101854No Hit
CATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAG5090.13552191913947575No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA5070.13498941651024401No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT5070.13498941651024401No Hit
GTTTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTC5050.13445691388101227No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT5040.13419066256639642No Hit
ACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCCGGAG4980.13259315467870122No Hit
CCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCACCT4950.13179440073485363No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC4940.13152814942023774No Hit
CCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATC4940.13152814942023774No Hit
GAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATC4910.13072939547639015No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA4830.12859938495946324No Hit
GGCAAATACAGCTCCTATTGATAGGACATAGTGGAAGTGAGCTACAACGT4820.12833313364484736No Hit
GTCTACCCTCCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGA4800.12780063101561565No Hit
CATAGGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCG4750.1264693744425363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA4630.12327435866714591Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCAC4610.12274185603791418No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA4560.12141059946483486No Hit
GTTTATATTCTTATCCTACCAGGCTTCGGAATAATCTCCCATATTGTAAC4530.12061184552098726No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT4440.11821558368944446No Hit
TCACTAGACATCGTACTACACGACACGTACTACGTTGTAGCTCACTTCCA4410.11741682974559686No Hit
ATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGATTTACGCC4380.11661807580174927No Hit
GTACTACACGACACGTACTACGTTGTAGCTCACTTCCACTATGTCCTATC4270.11368931134097475No Hit
GTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGCTGGCATCACTATACT4270.11368931134097475No Hit
GATTATGGTAGCGGAGGTGAAATATGCTCGTGTGTCTACGTCTATTCCTA4220.11235805476789541No Hit
GACGTAGACACACGAGCATATTTCACCTCCGCTACCATAATCATCGCTAT4200.11182555213866369No Hit
TCCTTGTACTATCCCTATGAGGCATAATTATAACAAGCTCCATCTGCCTA4190.11155930082404782No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG4180.11129304950943195No Hit
TCCTAGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAGAC4140.1102280442509685No Hit
GAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCAT4130.10996179293635262No Hit
CCCTTAGCAGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCAT4110.1094292903071209No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT4080.1086305363632733No Hit
ATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCCCTCTATT4070.10836428504865743No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT4050.1078317824194257No Hit
CTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAGGCTTCTCAAATCATG4050.1078317824194257No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC4040.10756553110480983No Hit
GCTCTAAGCCTCCTTATTCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCT4010.10676677716096224No Hit
CTCCACGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGG3950.10516926927326704No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG3940.10490301795865117No Hit
GGTTTATGGAGGGTTCTTCTACTATTAGGACTTTTCGCTTCGAAGCGAAG3880.10330551007095598No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG3860.10277300744172424No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG3820.10170800218326079No Hit
GTCTACGTCTATTCCTACTGTAAATATATGGTGTGCTCACACGATAAACC3810.10144175086864492No Hit
TGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTGTAGTA3810.10144175086864492No Hit
TTCATGTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCG3800.10117549955402905No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA3770.10037674561018145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)