FastQCFastQC Report
Tue 10 May 2022
EGAF00003646108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286116
Sequences flagged as poor quality0
Sequence length140
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAAT7360.2572383229179773No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT6380.22298648100770319No Hit
ATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTCATCAA6370.22263697241678201No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC5550.19397726796124648No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT5490.1918802164157195No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT5390.18838513050650785No Hit
GTATAGTACGGATGCTACTTGTCCAATGATGGTAAAAGGGTAGCTTACTG5340.18663758755190205No Hit
GGCCTATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCAT5040.1761523298242671No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC4780.16706510646031678No Hit
CACCATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTTCAC4630.16182247759649931No Hit
CATTGGGACAGACCTAGTTCAATGAATCTGAGGAGGCTACTCAGTAGACA4430.15483230577807602No Hit
CTATACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCCGCC4410.1541332885962337No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC4390.15343427141439137No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT4360.15238574564162788No Hit
GTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCC4350.1520362370507067No Hit
GTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAGGTCTG4300.1502886940961009No Hit
GTATTAGGATTAGGATTGTTGTGAAGTATAGTACGGATGCTACTTGTCCA4250.14854115114149505No Hit
CCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCATTC4210.1471431167778104No Hit
GTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT4140.14469655664136225No Hit
GTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAATGT4100.1432985222776776No Hit
ATCTTAGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCC4010.1401529449593871No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC4000.13980343636846596No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTT3930.13735687623201778No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG3890.13595884186833312No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC3870.13525982468649078No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3800.13281326455004264Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCTATTGATAGGACATAGTGGAAGTGAGCTACAACGTAGTACGTGTCGTG3760.13141523018635798No Hit
ACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCACAGCAGT3750.1310657215954368No Hit
GAATATGATAGTGAAATGGATTTTGGCGTAGGTTTGGTCTAGGGTGTAGC3630.12687161850438283No Hit
GTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGCCACCTACGGTGA3590.12547358414069817No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT3580.125124075549777No Hit
GGTATGGTCTGAGCTATGATATCAATTGGCTTCCTAGGGTTTATCGTGTG3550.12407554977701353No Hit
ATATAAAACCCCCTGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGA3510.12267751541332886No Hit
GGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCAACCGCCTTTTC3500.1223280068224077No Hit
GTCCTAATCACAGCAGTCCTACTTCTCCTATCTCTCCCAGTCCTAGCTGC3490.12197849823148653No Hit
GGTGAGAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCC3430.11988144668595953No Hit
ATATTATGCTTTGTTGTTTGGATATATGGAGGATGGGGATTATTGCTAGG3420.11953193809503837No Hit
GTGTAGTAAGGGTGGAAGGTGATTTTATCGGAATGGGAGGTGATTCCTAG3420.11953193809503837No Hit
GAATAGTGTTAATGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGG3400.11883292091319606No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT3310.11568734359490557No Hit
GTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGTACTACGT3300.1153378350039844No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA3270.1142893092312209No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT3200.11184274909477275No Hit
GTCCTAGCTGCTGGCATCACTATACTACTAACAGACCGCAACCTCAACAC3140.10974569754924575No Hit
GTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCCTCAGAATG3080.10764864600371878No Hit
GTAAAGAATCGTGTGAGGGTGGGACTGTCTACTGAGTAGCCTCCTCAGAT2980.10415356009450712No Hit
GCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATCAC2950.10310503432174362No Hit
ATATTACGGATCATTTCTCTACTCAGAAACCTGAAACATCGGCATTATCC2890.10100798277621664No Hit
GATATATGGAGGATGGGGATTATTGCTAGGATGAGGATGGATAGTAATAG2890.10100798277621664No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)