FastQCFastQC Report
Tue 10 May 2022
EGAF00003646136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293589
Sequences flagged as poor quality0
Sequence length140
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC5840.19891753437628795No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5130.17473406701204744No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA5090.17337161814645644Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4180.14237590645426088No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG3940.1342012132607148No Hit
TCGCTATACGGTGCACAAGTGCACACTTTGAAACGGAACTTCCCTATAGC3780.12875141779835075No Hit
GTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTGGGGTTT3730.127048356716362No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA3690.125685907850771No Hit
GTATTTATTGTCACTACCTCCCCGAATCGGGATTGGGTAATTTGCGCGCC3650.12432345898517995No Hit
GTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAATGGATGGCGCT3540.12057672460480467No Hit
GTATAATACCTTTATGGGGACCGAGGTTCGCGCTTCGGCAAGGATGCTGG3460.11785182687362265No Hit
GTATTATACAATAGGCTATAACACTCCCGAGGGAGCCACATTCCTACTGC3430.11682999022442939No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT3310.11274264362765635No Hit
CAATAAAGAGCATGCGGCTCCCCAGAGGGAAAGGCCCGGCCGAACCAGTG3220.10967713368007657No Hit
ATATTGCCTTCATCCATTCTCAAACTTTAAATATGTAAGAAGCCCTTGTT3200.10899590924728106No Hit
GTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCAAGATGT3180.10831468481448557No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3100.10558978708330354No Hit
GCTAAGGACTGTGTAACAACTCACCTGCCGAATGTACTAGCCCTGAAAAT3090.10524917486690577No Hit
GGGTAGAAACGATGCCCTGAGGAGTAGGCGGCCGTGGGGGTCAGTGACGA3080.10490856265050802No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC3020.1028648893521215No Hit
CTTGTGCACCGTATAGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGG3000.10218366491932601No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)