FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003646181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length140
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCATCCCACGTTTTACCCTCAGCACTACGATATCGTGCTTTCGATGA37.317073170731707No Hit
CCATGTCGTAAGCCTTGGCGATGCACTTGCCCGGATCGGTTTCCAGGTAT24.878048780487805No Hit
GTGTTGGTCAGCGAGATTTCCGAGGTCGAGATCAGGTACTGCTCGCCCTG24.878048780487805No Hit
ACCCGCGGCTGTCGGCGCGCGAGAACATCGTCTACTTCGGCCGCCTGCAG24.878048780487805No Hit
ATCCACGTGCAGATGCAGGGCACGGCCGGCCAGTCGTTCGGCGCGTTCCT24.878048780487805No Hit
GGTTCTCGAACCAGCTGTGCGCCTTGTTGGTCGCCAGCGAGTTGACGGCG24.878048780487805No Hit
GAGATGTACGCCCGCGGCCAGTCGCGGCAGGCGCGGGCGTACATCTCGGC24.878048780487805No Hit
GCCAATACCGATTCCTGGCGCATGTCGCTGCTGATGATGCTGGGCGTCTT24.878048780487805No Hit
CACCCGGGGCGGTGCGCCGCTTCCTGCGCCACTGGCAGCCGCGGCTCGGC12.4390243902439024No Hit
GATAAATCTTATCCTTTTATCTTCCTGAGTGGCGATACCCACCCGCGTCT12.4390243902439024No Hit
ATGTGGTGACGACGGCGGTGCGGATTGCCGCGCTGGATGCGCAGATCGCC12.4390243902439024No Hit
AATAAGAGCTATCTATGACAAACCCACAGCCAATATCATACTGAATGGGC12.4390243902439024No Hit
GGACGTTGCTCGACCCAACAGCAATGGTCCGCCACCCCATTTCACAAATA12.4390243902439024No Hit
GGCTATCCCCGTCTTGGTTGCACGCGAAGGCATCGATCACCAGCTGGTAC12.4390243902439024No Hit
CCAAGGAGAAGCACATGTTGAAGAAGCTGTTGGCTGCTTCGTTGATGTCG12.4390243902439024No Hit
GTCGAGCACATGCCAGAAGCGGTCGAGCACGATGGCCGGATACGGTGCCT12.4390243902439024No Hit
GCACCGCGGTGCTCGACGGCAAGGTCTACGCCCGCGACGAGGACTACACC12.4390243902439024No Hit
GTTTTGTTCTGTTATGTTTTGAGATTGCCCAGGCTGGAGTGCAGTGGCAC12.4390243902439024No Hit
GGTGAATTCTCGACGTAGGCCGAACAGATGCGGCACGACTTCATGCCACC12.4390243902439024No Hit
CTGGCATCCCACGCTCCAACTGAGGCATTGCCCGGCATCTCACTCAGCAT12.4390243902439024No Hit
AGCGAAAGGAGGTCTTCGCGGCGGGCACGCTCGTAGCGGCGCAGCAACCT12.4390243902439024No Hit
GTTGCCAGGCTGGAGTGCAATGGCACGATCTCGGCTCACTGCAACCTCCT12.4390243902439024No Hit
CGATGAATGCGACATCGCGGCGGCCGTCGGCCCGGCGGAGCTGGACGAAC12.4390243902439024No Hit
GGGCAGCAGCTCCTCGGTGTTGGAGAACATCTTCTTCTCGATGACGGTGC12.4390243902439024No Hit
GTCTATCGACAACACCAGCACGTGGCGCATGACGGGCAGCTCCGTGCTGA12.4390243902439024No Hit
GTCTAATAGTTGGGTGAATAGATTCATGTGTCTGAAGCCATACAACACTA12.4390243902439024No Hit
AGAGATATGGACCTCAATACAAGGCGCCACCAACTGGACGAGGGCATCGG12.4390243902439024No Hit
GAGCAGCAGCAACCGCGGGCCGTTTTCGCTGGAACAGAGAGAGCATGCGG12.4390243902439024No Hit
CTCCAGCGCAATCGCCCGCGCCACCATCTGCGCGCTCAGGCGCTCGGCCG12.4390243902439024No Hit
GGTTCACATAGGCGCCACCGCGGCCGAGGAAGGACGCGTAGCTGCGGTCC12.4390243902439024No Hit
ACGGCATCGGCGAGCAGGACGACATGCTGGCCGTGCTCGACTGTATGCGT12.4390243902439024No Hit
CATCAAATGATGCTTACGCTGAAGATTCGGGATGCTCGAGTGGAAGCTGC12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)