FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003646182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length140
%GC62

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATTCTCAGCGGCCATCTGCTTCGCGATGTGGATGTCAATTGGGATAGT37.317073170731707No Hit
CCTTCCACCACCGTGGTGGCGCCGGAGTTGCGCACCGCGAAGCGCTCGCC24.878048780487805No Hit
GACGATGCACTTGCCGCCGCCCGCCGGGCTCTTGAGCCAGCGCAGGGCCT24.878048780487805No Hit
GTGAAGAACCTGCACGACGCGGCTATCGCCGAGATCCTCAAGCGCAGCGG24.878048780487805No Hit
CTCCCGCCGTGGCGTGCAGACCCGCACCGAGGAACTGCAGGCCCGCCGCA24.878048780487805No Hit
TCCCCGGGCTGCGCGCCGACTGGCAGAAGCTGCTCGCCTCGCTGCCGCGC24.878048780487805No Hit
GATTCATCATGCCCAGCGCGGCTAGCGCGGCAAACCACAGCAGCATCACC24.878048780487805No Hit
CTTTCGGGCCACCACTCCACGCTGCTGCGCGACACCGTGCGCACGCTGTT24.878048780487805No Hit
ATCCTGTTCGCCGTGGATGATGATGGTGTCGGCCGGCACCGGGGCGACAT12.4390243902439024No Hit
GGGCGCAGTGGCTCATGCCCATAATCTCAGCAATTTGGGAGGCCGAGGGG12.4390243902439024No Hit
GCGTAAGCACCTGATCATCTTTGCCAGACAAAAACAGGCGCACATACTCG12.4390243902439024No Hit
GATAAGCGCATTAAAGACGGGGAGGAAAGACCGGAGGATCATTCATGAGC12.4390243902439024No Hit
AAACTAGACTTTGCGCCGCGCACGACTTTTGCGCATCATGAATAGCATTG12.4390243902439024No Hit
TACTTCTTCTTCGCGTCCTTACCGTCCTTCTTGGCGGCGGCCGGTGCCGG12.4390243902439024No Hit
GCCACGTCGACGCGCGGCACGCCCATGGTCAGAAGGCGTTCGGCATCGGC12.4390243902439024No Hit
GAGTTGCGAGCGCAGGTACCAGCCGTTCGCGTCGCCTTGCCCGCCGCGAT12.4390243902439024No Hit
GTCGATCGCCATGTCCAGGGCCTGACGCACTTCGACCTTGGACAGCGCCG12.4390243902439024No Hit
CACGGCATATGAGCAGCTACATAGCGCCTCGGAGAATCTGTTCAAGCGCT12.4390243902439024No Hit
CCAAGAGCTACCAGGAGTACCGCGACTACGCCGGCGTCGACGAAGGCATG12.4390243902439024No Hit
CTTGCCACCGGCATCGACGCCGAGCGCCTGCCGGATGGGCTTGGGCAGCG12.4390243902439024No Hit
GGCGGCCGTCGTGCAGGACATTGCCGCCGACCATGGCGGCGAGGCCTTCG12.4390243902439024No Hit
AATTAAAGGAAAGTCTGACATGAACAAAGGTGATAAATAACTAAAGTTTC12.4390243902439024No Hit
CTTCCCGCAGTTGCTGCGCTTCTGGCGTACGCGGCGCGGGTATAGCCAGC12.4390243902439024No Hit
TCTGAACCCCAACAATGGAGTTCAAAACTCACCGATATGAAGTTCTGAAA12.4390243902439024No Hit
GATCACAAGGTCAGGAGATCGAGACAAGCCTGGCCAATATGGTGAAACCC12.4390243902439024No Hit
GACTTTGTTGAAGTTGCCTATCAGCTTAAGGAGATTTTGGACTGAGACAA12.4390243902439024No Hit
TGCCCGAGGCGTAGCAGGCTCCCAAAGATAGTCCCCCGAAGGCCGCAGGT12.4390243902439024No Hit
GGCCTACAGCACCGCTCAGCTGGACCTGGTCAACGGACTCAATAAGTGAT12.4390243902439024No Hit
GCCCGAGGCGCTGTGCCGCGCGGTCGAGGCCGCGGCGGGTGGCGCCATCG12.4390243902439024No Hit
CACCTGGCAGAGCAAGACCCAGTACGACATCGGCCAGTTCCCCGACACCA12.4390243902439024No Hit
TGCCGCTGCTGCGCAGCCTGTTGCATGAGCGGCGGCAACGTGGCGTCGGT12.4390243902439024No Hit
ACCAGGCCTTGATCTGCGCGGCACTCACGGGCGTGCTCTTCGACCACGTG12.4390243902439024No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)