FastQCFastQC Report
Tue 10 May 2022
EGAF00003646453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225241
Sequences flagged as poor quality0
Sequence length140
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT6070.2694891249816863No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC5220.23175176810616183No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC4980.2210965144001314No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4850.21532491864269826No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC4380.19445838013505534No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT3950.17536771724508415No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT3900.17314787272299448No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA3900.17314787272299448No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT3530.1567210232595309No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA3480.1545011787374412No Hit
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG3120.1385182981783956No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA3100.13763036036955972No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA2880.12786304447236516No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG2880.12786304447236516No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC2810.12475526214143962No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC2800.12431129323702168No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG2690.1194276352884244No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATG2670.11853969747958853No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2640.11720779076633474No Hit
GCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAA2580.11454397733982713No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC2560.11365603953099124No Hit
GAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGG2520.11188016391331951No Hit
TATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT2470.10966031939122983No Hit
GCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAATTTGAAGGCG2460.1092163504868119No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA2450.10877238158239397No Hit
GAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGG2440.10832841267797604No Hit
ATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAGATTTT2440.10832841267797604No Hit
CTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT2410.10699650596472222No Hit
GCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCA2350.10433269253821462No Hit
TAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCAT2320.10300078582496082No Hit
ACTTTAAGCTGTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTT2270.10078094130287114No Hit
CTTTTTAAAAGATCGCCTTCAAATTATTTTAATCACCTACAACTTTTAAA2260.10033697239845321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)