FastQCFastQC Report
Tue 10 May 2022
EGAF00003646454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences225241
Sequences flagged as poor quality0
Sequence length140
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT6020.26726928045959664No Hit
TCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCAC5590.24817861756962542No Hit
CAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATGCGTTAACTAGGC5260.23352764372383356No Hit
CTTCTATCTTCTCCAAATTGTTTCATCCTACCACTCCCAATTAATCTTTC4750.2108852295985189No Hit
GGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTAGAT4690.20822141617201131No Hit
GACTTAAACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA4070.18069534409809937No Hit
GTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTT3790.1682642147743972No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT3690.16382452573021786No Hit
GTTTAAGTCACCTTCATTTTAATCTAAAAGCATTGCCCTTCTATTGGTAT3660.16249261901696405No Hit
GACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACA3370.14961752078884394No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA3120.1385182981783956No Hit
GTATTAATTCGGGGCTCTGTAGTCCTTTCTCTCAATTTTCTTTTAAATAC2980.13230273351654448No Hit
TCCTACCACTCCCAATTAATCTTTCCATTTTCGTCTGCGTTTAGTAAATG2900.12875098228120102No Hit
CCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCCAA2850.12653113775911137No Hit
TTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTTCTCC2820.12519923104585753No Hit
GATTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTG2810.12475526214143962No Hit
CTAAGATATTGCTTAGCGTTAAGTTTTTAACGTAATTTTAATAGCTTAAG2730.12120351090609614No Hit
GTACATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2700.11987160419284232No Hit
GAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGGAGG2670.11853969747958853No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA2670.11853969747958853No Hit
GGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAAT2630.11676382186191678No Hit
TATGAAGACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCT2590.11498794624424506No Hit
GATAAAAGGTTTCTAAAACATGACGGAGGTTGAGATGAAGCTTCTTCATG2570.1141000084354092No Hit
CTTTTAATCACCTTCGGTTTAATCTCTTTTTAAAAGATCGCCTTCAAATT2520.11188016391331951No Hit
GCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCA2510.11143619500890159No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC2490.1105482572000657No Hit
GAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACATGACGG2480.11010428829564776No Hit
TAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGCGTCAT2440.10832841267797604No Hit
GCTTTAAATGACGCAATTCTCCCTGCGTCATGGATTTCAAGGTCTTTTAA2370.1052206303470505No Hit
CTTTTTAAAAGATCGCCTTCAAATTATTTTAATCACCTACAACTTTTAAA2300.10211284801612495No Hit
TCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGAGAGAAAGGACTA2300.10211284801612495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)