FastQCFastQC Report
Tue 10 May 2022
EGAF00003646723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722172
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC24900.34479320715840545No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT20920.289681682480074No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG17200.23817040815761345No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT16130.22335399323152932No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT15650.2167073771899215No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT15320.21213782866131614No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG13230.18319735464681544No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT13010.1801509889610785No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT12380.17142730540646828No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT11870.16436527586225996No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG11380.15758018865311865No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC11290.15633394814531718No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC10880.15065663027644383No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA10730.1485795627634414No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG10720.1484410915959079No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT10520.14567166824523797No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC10390.14387154306730254No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT10180.1409636485490991No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC9380.1298859551464194No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG9310.12891665697368493No Hit
ATCCTGTTCTTGCCCCGCAAGCGCTCTCAGGCAGAGCTATGTGCAGACCC8900.12323933910481161No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG8880.1229623967697446No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG8630.1195006175814072No Hit
CTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTT8530.11811590590607224No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC8480.11742355006840477No Hit
GTACATGGGACAGACCCCCAACTTGCAGCTGCCCACCTCACCCTCAGCTC8450.11700813656580426No Hit
ACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCA8040.11133081869693093No Hit
CCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCT7780.10773056834106003No Hit
CAAGAGGACTGAGCGGTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGC7550.10454573148778962No Hit
TACTCACCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCT7380.10219172163972018No Hit
GCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGGGAATGCAGATGGGGT7270.10066853879685171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)