FastQCFastQC Report
Tue 10 May 2022
EGAF00003646724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722172
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC23370.32360711852578056No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT19250.26655699750198014No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT17910.24800186105249167No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG16670.2308314362783381No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT16320.22598494541466577No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT15630.21643043485485453No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT12790.1771046232753416No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT12280.1700425937311333No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG12100.16755011271553036No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG11870.16436527586225996No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC11490.1591033714959871No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG11460.1586879579933866No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT11060.15314911129204678No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC10340.14317918722963505No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT9930.1375018693607617No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC9860.13653257118802722No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT9710.1344555036750248No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA9700.13431703250749127No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC9500.13154760915682137No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC9450.13085525331915387No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG9020.12490099311521356No Hit
CTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTT8950.12393169494247908No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG8590.11894673291127321No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG8410.11645425189567028No Hit
GTACATGGGACAGACCCCCAACTTGCAGCTGCCCACCTCACCCTCAGCTC8380.1160388383930698No Hit
ATCCTGTTCTTGCCCCGCAAGCGCTCTCAGGCAGAGCTATGTGCAGACCC8340.11548495372293581No Hit
CCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCT7760.10745362600599302No Hit
TACTCACCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCT7430.10288407747738766No Hit
GCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGGGAATGCAGATGGGGT7370.10205325047218668No Hit
CAAGAGGACTGAGCGGTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGC7230.10011465412671774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)