FastQCFastQC Report
Tue 10 May 2022
EGAF00003646725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003646725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences747186
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTTAGGCTGCTCCATCCCAGCTATCCTGTTCTTGCCCCGCAAGCGCTC25980.34770458761272294No Hit
GCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATTCCCGCCAAGGT22100.29577641979373276No Hit
CTCTGGACCTGGCCTGCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGG17530.23461360357394279No Hit
GTCCTGCTGCCTCCTCTCATGCTCCCTGGGAGACTCTCCCTCCTCGGTCT17360.2323384003447602No Hit
TCCTTATCCTGGTTCTGGCCTTTGGCATCCCCAGGACCCAAGGCAGTGAT16610.22230073903954303No Hit
GGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAGAGTGT16260.21761649709710834No Hit
GTACTGGGGAGCCGTATCAGGTCCAGGGTCCTGATGATTCTCCTTCAAGG14390.19258926157610018No Hit
CTTTAGGGGTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTT12850.1719785970293876No Hit
CCCCAGGACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGT12750.17064024218869198No Hit
CCCTGGGAGACTCTCCCTCCTCGGTCTCTCTGGACCTGGCCTGCTGTGGG12300.16461764540556167No Hit
GTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTTGCCAGTCTTGGAGGC12020.1608702518516139No Hit
GTCTGTGACCGCTCAGTCCTCTTGCAGCCTTTGGAGCCCTTTCCTTTCTT11960.16006723894719654No Hit
GTCTTGGAGGCCCCCCTGTCCTTCCTGCAGCCCTGGGCTGGTTTCTGTGG11820.1581935421702227No Hit
ACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTTCT11210.15002957764197938No Hit
CTCTCAGGCAGAGCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGC11120.14882505828535333No Hit
GTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCAAAGGAAGATT11090.14842355183314462No Hit
GCTATGTGCAGACCCAAAGGAGCTCTGGGTGCAGCAGCTGATGCAGCATC10610.14199944859780564No Hit
CCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGGAGACCCCA10340.13838589052792744No Hit
GGTTAAAGCAGGAGAAAGAGTGTGGCAAGGCCAGCATGGGGTGGCTGCTC9790.13102493890410152No Hit
GTCCTGATGATTCTCCTTCAAGGGGACAGTCCTGCTGCCTCCTCTCATGC9510.1272775453501538No Hit
CTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGG9350.12513617760504078No Hit
GTCACAGACCCCTAAAGGGCCATAGCCCAGTGAGCAGCCTGGAGCCCTGG9230.12353015179620602No Hit
CTCCAAGACTGGCAAGAAAGGAAAGGGCTCCAAAGGCTGCAAGAGGACTG9200.12312864534399734No Hit
CCCTCTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCT9150.12245946792364952No Hit
GTACATGGGACAGACCCCCAACTTGCAGCTGCCCACCTCACCCTCAGCTC8510.11389399694319755No Hit
CTACCACAGACATGGCTCAGTCACTGGCTCTGAGCCTCCTTATCCTGGTT8390.11228797113436279No Hit
ATCCTGTTCTTGCCCCGCAAGCGCTCTCAGGCAGAGCTATGTGCAGACCC8380.11215413565029324No Hit
GCTGTGGGCAGCTCAGCCATGCAGGGTAGAGCTGGGAATGCAGATGGGGT7840.10492701951053686No Hit
ACCCAAGGCAGTGATGGAGGGGCTCAGGACTGTTGCCTCAAGTACAGCCA7680.10278565176542387No Hit
ATGCAGGGTAGAGCTGGGAATGCAGATGGGGTGGTTAAAGCAGGAGAAAG7620.1019826388610065No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)