FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATGCATTTATCTCCACTAGACTATAAGCTATTTGAGGAGAAGAATAA34.477611940298507No Hit
GCACCTGCGTGAGCCGGCCCGCGTCGTCCCACGCCAGCAGGACGTCTTTG34.477611940298507No Hit
CTATCACATAAGGCATCATTATAACTAAAAATGGGATATATTCCTTATAA34.477611940298507No Hit
TTGTTTGGAGACAGTGTAGACTCTGATTATTTAAGTAATCGTAATGATTA22.9850746268656714No Hit
GCGGAAGGATATGGTAATCGTGGTTCTTTGCGTGCGCCATGAGCGGTCCC22.9850746268656714No Hit
GCCTTGGGGTTGAAACAACCTTAACCTATTCTCAAACTTTAAATATGTAA22.9850746268656714No Hit
TTCATGTGCAGCGGCAGCGGGTTGCGCGAAACCTCTGCAACCTTTAGCCG22.9850746268656714No Hit
ATCCACAAGATGGAAGAAGGCGTCCTTCGCCGAGGAATCACGGAAGATAC22.9850746268656714No Hit
GGACACTAAGGTGTAGGGGAGGAAAGGGAGGTGTGTGTGCAGAGGTGTGT22.9850746268656714No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA22.9850746268656714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA22.9850746268656714Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC22.9850746268656714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTTGATGTCGCGCATCGCGAACGCCGTGGTCTGGCTCCAGGCCAGTGC22.9850746268656714No Hit
ATATCATTAGTAAGGTGTCACTCTCCTCCCTCACTACCATGCAAGCTGAA11.4925373134328357No Hit
TCAAGACATAGGCTGTCTGCTTCGGCCGCAGCACGACATCGATCGTGCCT11.4925373134328357No Hit
CTCCTCTAGGTCTTTTTCTTCGCAAGTCAAAACCTAAGCGAGACTGGGCG11.4925373134328357No Hit
CGGGTGGCGTGCGCCTCCCACGCGGCGGCGCGGGCGGCGTCGCCGCGGGC11.4925373134328357No Hit
TTTTTGCAGTGTTGGCATAGCTCGAATGACAAGCTAACACTGCTACCGTT11.4925373134328357No Hit
TCGNTGGTCGCCGTATCATTCCCATGTACTCTGCGTTGATACCTGTCTCT11.4925373134328357No Hit
GTCGGCTGATCGTGGTTTTACTAGGCAAGACTAGCGTACGAGCACTAAGG11.4925373134328357No Hit
GTGACTGTGAGATGCCGTCTACGAGTGGCACGAATCGCACGGATGTTTGG11.4925373134328357No Hit
CGGCGGAGTCGTACGACGCAGTGGCCGAGGCCATCGGCGCAAAGCTCGCG11.4925373134328357No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CTACATCACCTCGCAATATCCCTACGGCTTCCGCGCCCAGCAGCTCCGCA11.4925373134328357No Hit
GCAAACAGGAGGTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAA11.4925373134328357No Hit
CCCGTAGACGGCGGCTTCTCCATTTCGGGCTGAAGCCCGCGGGCCGTGTA11.4925373134328357No Hit
GAAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTGTCTCT11.4925373134328357Clontech SMARTer II A Oligonucleotide (95% over 23bp)
GTTCCGAAATCGGTGAAGTAGTCGGTGGTGTCTTTCTCGAGAATGTAGAT11.4925373134328357No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CAGCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAA11.4925373134328357No Hit
CCGCATGGAGGACGCCGGGCGGTTCAAGAAATGGCTCTTTGACCACTACA11.4925373134328357No Hit
GAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCG11.4925373134328357No Hit
GTACTTTTTTTTTTTTTTTTAGGGTTAGTCGTATTTGGGTTAGCGGTATT11.4925373134328357No Hit
GTTTAACCTCTGCCGCGATCTCCGGCGTCGACGGGCGTCTGACGCCGGAG11.4925373134328357No Hit
GGTAAGAAATGCCATGTTTGCAACAGCAAGGATAACTACCAAAACTCGCC11.4925373134328357No Hit
GTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCTTGTCGGCTAT11.4925373134328357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTT11.4925373134328357Clontech SMART CDS Primer II A (96% over 26bp)
GTTTTTGTGTGACGCTGGTCAGTACTTTTCCGTCTGCTGTTTTCGTCAGA11.4925373134328357No Hit
GCAAGGTAGTGATGCTGGTAGCGCTCCCAGGCTCGGTCGAACAGCGCTCG11.4925373134328357No Hit
GTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGG11.4925373134328357No Hit
GGAAGCAGTGGTATCAACGCAAAGTACATGGGAAGCAGTGGTATCAACGC11.4925373134328357Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTATTGTCTTGTTCAAACTTGCGAAAAATCCGGTACATGTTAAAAAATA11.4925373134328357No Hit
GCTTAAAAGACCGCTACATCGATAGTCTGTCTGGTGGGCAGCTCCAACGT11.4925373134328357No Hit
CTATTTATCAATGATACAGAAGTTGCTCATGGTCGCATTGAAAAAACCGT11.4925373134328357No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTATAC11.4925373134328357No Hit
CGACAGCGCCCGTGCGCGGCTGCGCGTTCTGGTAAAACGCATCCTGCGCA11.4925373134328357No Hit
TTTACTGGACTCGCAACGTGGGTCCATCAGTTGTCCGTATACCAAGACGT11.4925373134328357No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC11.4925373134328357Clontech Universal Primer Mix Long (96% over 25bp)
GTGGCGATGGACGTAATCGAGGCACGGCTGCGCGCGAGTTTCGCGCGGCA11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)