FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGG34.477611940298507No Hit
TGCGACCACATCGAGCACCTGTCGGCCGAGGGCATCGAGGCGATGCGCCA34.477611940298507No Hit
GGTACATGCCTGTAGTCCTAGCTACTTGGGAGGCTCTCTTGAACCTAGGA34.477611940298507No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA34.477611940298507Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCTACTCGAGCCTGGGCCTCAAGGCCAAGATGGATGCTGGGTAAGCTG22.9850746268656714No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT22.9850746268656714Clontech Universal Primer Mix Long (96% over 26bp)
GCCACGATCCGTTCGACGTCGCGCTGGACCAGATGCGGGTTCTCGTCGGC22.9850746268656714No Hit
GTCTTTACATTGCCTGTCTTGTATGCCCTTGAAGAGGATAGTCATGCGGG22.9850746268656714No Hit
TAACTAAGTTTTGTTTGTACAGGTTCAATTACTTCGCCAACATGTGCTTC22.9850746268656714No Hit
CCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAAC22.9850746268656714No Hit
GCCCTCCACCATCACCGTGAAGGTGAAAGGCACCGACGGCGCGATGACGC22.9850746268656714No Hit
TACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACT22.9850746268656714Clontech Universal Primer Mix Long (96% over 26bp)
GACCAGCATGGAACTGAAGATCGGCGAAAACGGCCTCGCCTTCTACGAGG22.9850746268656714No Hit
CTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGA11.4925373134328357No Hit
TAATTATTCGGCGGCAGGTAATTATCTTCAGCTGTGACCATATCTTCGAA11.4925373134328357No Hit
CCGTAATACTTCAGATCAGTTAAGTAGGGCCATATGCCTCGGGAATAAGC11.4925373134328357No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA11.4925373134328357No Hit
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTTCCTGTCTCT11.4925373134328357Clontech SMARTer II A Oligonucleotide (95% over 23bp)
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA11.4925373134328357No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG11.4925373134328357No Hit
CTCGGATGAAAAAGCAGAATTATCGCGCATCGTTAGAAGCTTTCAAACAA11.4925373134328357No Hit
CGTCGATCTCGACGGCACCATGGTCGACACGCTGGGCGACTTCGCCGCCT11.4925373134328357No Hit
GGGTGATGAACACCGTCGCCTCGGTCTGGCCGTAGAGCTGCTTCAGGTTG11.4925373134328357No Hit
TGTAAGGAATGCTAGTTTATTAATCAATGAAAAGTTGATTAATAAATCTA11.4925373134328357No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GACCAAGACCTCGCGCGACGATTTCCTTCATGATTTCATTCGATCCGGCA11.4925373134328357No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CGAGCGGACCGGCCCGCACGGGTTGCCGCTGATCCTCGGCGGCGACTGGA11.4925373134328357No Hit
GCGGGGGCCAGCAGGTTGATCTTCATCTCGACCGTGACCACCTCGGCCCC11.4925373134328357No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT11.4925373134328357No Hit
GCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAA11.4925373134328357No Hit
TTTCTTCATCGCTCCGAGAAAGTTGAGTTTCCTAAAGTCCAACGTGCTGA11.4925373134328357No Hit
CTGCGCAGTAAATAATACACCCAATTCGTCTCCACACTAGAAGTCGACGA11.4925373134328357No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAA11.4925373134328357No Hit
CAGCATGCCGTCGCTGGGCGAGAACGAAGGGCGGATCTACTTCTATCGCA11.4925373134328357No Hit
GCATCGGTACACGGCGACAGGTCCACGGTCTGCAACTTGTCGAGGGTGCG11.4925373134328357No Hit
GTGCGCGAGGTCGCGGTAGGGCAGCAGCGTGGCGACGGGTCCGAACACTT11.4925373134328357No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
GATGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CGCCGGCGCGTTCGGCGGCGGCCGCCGTGCGGGCGGCCAATTGGTGGGCG11.4925373134328357No Hit
GTGAGGCTCAACAGGAGAGTTACGAAGATAGCCCAAGGAAATATCGACTA11.4925373134328357No Hit
GACACGTTGGAGCTGCCCACCAGACAGACTATCGATGTAGCGGTCTTTTA11.4925373134328357No Hit
ATCACGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCT11.4925373134328357No Hit
CTTCAGATCGTAGCTTCCGTCACTGTCCGATTTTCGGGGGGTAGCTCACT11.4925373134328357No Hit
AACTAAAACTGATTAAGGTTAAAGAACCCAAAGATAATTTTGTCGGTATA11.4925373134328357No Hit
CCCAAGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT11.4925373134328357Clontech Universal Primer Mix Long (96% over 26bp)
CTTTAACACCACGACTATGCAAAAACTCCATTAACTCAGGCAAGTCAGCT11.4925373134328357No Hit
GAAGAGCGAGGAAACGATCTGCCTGGGCGAGAACGGATCGTCGTGCCACC11.4925373134328357No Hit
GTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGACTGTCTCT11.4925373134328357Illumina DpnII expression PCR Primer 2 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)