FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA36.521739130434782Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTCGGCGAGGAGGAGTTCTTCTCGGGCGGCCTGTCGATCCGGGCCACGG36.521739130434782No Hit
GTATCAACGCAGAGTACATGGGTGCGGGACATGAAAAAACAGTGCTTCGG24.3478260869565215No Hit
CTGTCGGCCTGCTCGACGTCGACGCGAACGAATTGGCCCTGGTCTACCGG24.3478260869565215No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTT24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
CTCGTGCGCCATCGGGTCGGGCATGGGCGCGGGCGTGCTGTCGACGCGGC24.3478260869565215No Hit
ATATCATTAGTAAGGTGTCACTCTCCTCCCTCACTACCATGCAAGCTGAA12.1739130434782608No Hit
CATCACAGGGCTGCTGCTGGCCGGCGGCCGTGGCTCGCGCATGGGCGGCG12.1739130434782608No Hit
TGTCGCTCTACACCAAGCGTCTCGAGGCCGGGAAGTTCATCTGGCCGACC12.1739130434782608No Hit
CATTGAGGTCGAGTATAATTCAAAATCAATTATTTCTTATCTCTTCTTTT12.1739130434782608No Hit
TACCAGACCACCGCCTGGCCCGCGACCGCGCCGAACAGGGCGAGGAGCAC12.1739130434782608No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGTTTATACCACC12.1739130434782608No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CGGCAGGGCCTCGGGCTCGAGACCCACGTCTTCTTCACCTTCTACTGGCC12.1739130434782608No Hit
ACCGAGGCTCGCGACGCGAGCGGGTCGTCGCGCCGGCACAGCAGCATGAA12.1739130434782608No Hit
CCCATGGCACGCGCCTCGCGGAACACGCGGATCCACTCCGCGGTGCCGAG12.1739130434782608No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG12.1739130434782608No Hit
CGCCAGAAGACCGTGCGCACCGTTCCGATCGGCCACGCCGCAACCCGCGC12.1739130434782608No Hit
GCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGT12.1739130434782608No Hit
GGGCGATGATGCTCAGCTGCGTCCCCAACTTCGCCTTCACGATCGGCTAC12.1739130434782608No Hit
GTCCCAGCCGCCATCGAGCCCCGCCGCGAACCCGAGCGCCGCGGGCGCCA12.1739130434782608No Hit
ATCTACAACCGGCTGGCCAGGATTCGGTCGGTTTTGGCCGCGGAACCGCT12.1739130434782608No Hit
GCGGTCGGTTTCCAGTTTCGTTTCCCCGGGACCTCTTGCCCCAATTCCTC12.1739130434782608No Hit
CTAGCAAAATTAATTTGAAAAAGTTTTGCGTCCGGTTGAAGTAATTACAT12.1739130434782608No Hit
GTTGAGAAGACAGCACCAAGAGTAGTTTCCGTGGGAATTCGAGAAAATAG12.1739130434782608No Hit
GCATTAAAACAATATGGTCATATGATTAATTTTGAAGAAGTGATGCTTGA12.1739130434782608No Hit
GATGTAGAGTCTGCCCCACCTGAAGGAGCAGACGAATGAGGAAAAGCCGT12.1739130434782608No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TACAGTAACCAGGCAAGAGTTCAATCGCTTAGTTTCGTGGCGGGATTTGA12.1739130434782608No Hit
CCCGTAGACGGCGGCTTCTCCATTTCGGGCTGAAGCCCGCGGGCCGTGTA12.1739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACAACAGATTCCCATGAACTCTGCGT12.1739130434782608No Hit
ATGTAATACTGATAATCCTCAGTTGTTAATGATACCCAATCCGGCAAAAG12.1739130434782608No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAT12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GGTCTTTGCTTACCTTCCGGTTCTAACCTTACAGATACAGACTTAAAACG12.1739130434782608No Hit
CCGCGACCACGCCCGCACCCATCTCGGCTGGGCGACCGCGGTCAGCTACA12.1739130434782608No Hit
TTCTGTACCTTCACCCAAAAATGAGTTGCAACTTAACTTACCTCCATGTT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)