FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length146
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATGCCCTCGATGCCGATCTGGGCGTCGGACTTGCCGAGCGACAGCACG36.521739130434782No Hit
GCTTCATAGTCGTCGACAACGTCCGCGGCGCCGAAGGTGCTGTTCGCGAG24.3478260869565215No Hit
AAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTT24.3478260869565215No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT24.3478260869565215Clontech Universal Primer Mix Long (96% over 26bp)
CGCATGATGCGGCCGAGCCGCTGCATGGCCTCGATGTCCAACTGCTTTCG24.3478260869565215No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GACCTGGCCGGTGCGCGGCTCTTCGACCGAGACACGCGGCATGTCGAACT12.1739130434782608No Hit
ACGCAGAGTACATGGGATGGCGCCACCGCGTCTACACTCTTTGCCTACAC12.1739130434782608No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA12.1739130434782608No Hit
CCCCCAGTACCCCACCATAGTTGAGCCTGGCGGTTGCCGTCGATGACCAC12.1739130434782608No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
GCCAGTAGAAGGTGAAGAAGACGTGGGTCTCGAGCCCGAGGCCCTGCCGC12.1739130434782608No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
CAATATACCGCTAACTAAGCTTGATTTCTTTTGCATCGCCCACTAATGCT12.1739130434782608No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGG12.1739130434782608No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.1739130434782608Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATTTTCGAGTCCCGTTTGATGTCTAAGCGGTAATGACCAATAGCTTGCAT12.1739130434782608No Hit
CTGTCGGGCCAGACCGGCGCGCCCAGGGCTTCGTATTCGGCGAGGTTGCG12.1739130434782608No Hit
GCTCTGAGCCGTGGCCATAGTGTTCCCGCCGCAGCCTGGCGATCTCAAGC12.1739130434782608No Hit
CCTCTGGCCGACCGCGACGTGGAGCACGGCCTCGAGGTGGCGAAGCAATG12.1739130434782608No Hit
GAATTGATGGCGCCTGCTTCCAAATTAAACAGGACTTCCGCAAAAATTTT12.1739130434782608No Hit
GTAATTCCTGGAGGAATAGGTACCAAGAAAAAACGAACCTTTGGGTTCCA12.1739130434782608No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGTGACGCAGTGA12.1739130434782608Clontech Universal Primer Mix Long (96% over 26bp)
TCGCCGACGTGTTCACGCTCGGCAATGCGGCGTGCGGCGTTGGGGCGGTC12.1739130434782608No Hit
CACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAAC12.1739130434782608No Hit
ATTTATTTACAACAAAAGCAATTGGTAAAGGTACGGGTTTGGGATTAGCA12.1739130434782608No Hit
GAAGCAGTGGTATCAACCCAGAGTACATGGGGTGGTATCAACGCAAAAAA12.1739130434782608Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTTAACACCACGACTATGCAAAAACTCCATTAACTCAGGCAAGTCAGCT12.1739130434782608No Hit
TGCGAATGCCGTTCATACGATTCTGCTGCCGAGGCCGCCGCAGCGACCGC12.1739130434782608No Hit
CATAAATACAATATAAGCAATTCAACTAATTAACGAGTAAACGCTCAATA12.1739130434782608No Hit
GAAGAGCGAGGAAACGATCTGCCTGGGCGAGAACGGATCGTCGTGCCACC12.1739130434782608No Hit
GTGTGGATCACCCGCTGGACGTAGCCGGCGTCGAAGTCCATCAGCGGCAC12.1739130434782608No Hit
ATTGCCAACAGACTATTACAAGCGACAAGTTGTCCAGGCACATCAAAAAT12.1739130434782608No Hit
CATTTGGGAAGTGGCTTTTACGATATCTTTATCTCCATATGTACCACTAT12.1739130434782608No Hit
GTCGAGCGCGGCGGCGGGGTCGACGCCCGCCAGCGCGGCGACCTCCGCGA12.1739130434782608No Hit
GCGCTGGGCGAGGCGGCGTTCGCCGATTGGGGCTGGCGGGTGCCGTTTCT12.1739130434782608No Hit
CTTCAGGACGACATTGTCGAGCATCGTGTCGGCCAGCAGGCGCTTGAGCT12.1739130434782608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)