FastQCFastQC Report
Tue 10 May 2022
EGAF00003647209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22961
Sequences flagged as poor quality0
Sequence length146
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA1010.43987631200731675No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA1000.4355211009973433No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT970.422455467967423No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT960.41810025695744957No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA940.40938983493750275No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT930.4050346239275293No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA920.4006794129175558No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG880.38325856887766213No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA860.37454814685771526Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGC750.32664082574800746No Hit
GTACATGGGAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCG730.3179304037280606No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT700.30486477069814033No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC700.30486477069814033No Hit
CATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATA700.30486477069814033No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT650.28308871564827315No Hit
TCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTG650.28308871564827315No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT610.2656678716083794No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT590.2569574495884326Clontech Universal Primer Mix Long (96% over 26bp)
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC580.2526022385784591No Hit
CGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTG560.24389181655851225No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT550.23953660554853884No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC540.23518139453856537No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC510.2221157615086451No Hit
TATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTGTCCA510.2221157615086451No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT490.21340533948869825No Hit
CTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCATAATGTGA490.21340533948869825No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG470.20469491746875138No Hit
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT470.20469491746875138No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA460.2003397064587779No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA460.2003397064587779No Hit
TAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAG460.2003397064587779No Hit
AAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAG430.18727407342885763No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT430.18727407342885763No Hit
GTACATGGGAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTCGCCCGAT410.17856365140891076No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC410.17856365140891076No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG410.17856365140891076No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG400.17420844039893732No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT390.16985322938896388No Hit
TATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTG390.16985322938896388No Hit
TATCAACGCAGAGTACATGGGATTGGCAACAGCAATTGAAGTGAAGATGA390.16985322938896388No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT390.16985322938896388No Hit
ATTCAAGACGTTAACAGTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTT370.16114280736901704No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC370.16114280736901704No Hit
ATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCC370.16114280736901704No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG370.16114280736901704No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT360.1567875963590436No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG360.1567875963590436No Hit
AAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTT360.1567875963590436No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT350.15243238534907017No Hit
GTACATGGGCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC350.15243238534907017No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC350.15243238534907017No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT350.15243238534907017No Hit
GTATAAAATCAGGCAGTTTTTTTTTTGATCACGTTTATTGTAAGCCGTCA350.15243238534907017No Hit
TCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGG340.14807717433909673No Hit
ATTAAATGCAGAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCG340.14807717433909673No Hit
GTATCAACGCAGAGTACATGGGATTGGCAACAGCAATTGAAGTGAAGATG340.14807717433909673No Hit
ACGCAGAGTACATGGGATTGGCAACAGCAATTGAAGTGAAGATGATTGAC330.1437219633291233No Hit
GTATCAACGCAGAGTACATGGGAGATATTGAGGCTGCTTCGTGTCGGCAC330.1437219633291233No Hit
ATCCCGGAAGATACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT330.1437219633291233No Hit
CATATAACCACATTCAAATTGACCTCCCTCAGGAAGCTAAGAAATACTAT330.1437219633291233No Hit
ATACTATCTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGA320.13936675231914988No Hit
GAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTGTCTTCCGATGCAGTG320.13936675231914988No Hit
CTCAAGCGTTGAGCGGAATGCAGCAATCAATGTCGTCGGAAGATCCTGAA310.13501154130917645No Hit
GCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCAC310.13501154130917645No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGA310.13501154130917645No Hit
GTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCTTCTTTC310.13501154130917645No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC300.130656330299203No Hit
GGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGATATTAG300.130656330299203No Hit
GGGTAAAACGCAAGCACCGGCTGTCGAGTTGTACGGCCGTTCAGCCACGA300.130656330299203No Hit
CACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTG300.130656330299203No Hit
ATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTCAAGCA300.130656330299203No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA300.130656330299203No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT300.130656330299203No Hit
TCACTATGTTGACCAGGCTGGTCTCAAACTCCCAACCTCGTGATCTGCCT300.130656330299203No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA300.130656330299203No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG290.12630111928922955Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATGGT290.12630111928922955No Hit
GTATCAACGCAGAGTACATGGGACCTGAAACGGGCATCGTCGCTCTTCGT290.12630111928922955No Hit
GTACATGGGACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTT290.12630111928922955No Hit
GTTCTTGGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCG290.12630111928922955No Hit
GATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTTCCGCCCCTGG290.12630111928922955No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC290.12630111928922955No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC280.12194590827925612Clontech Universal Primer Mix Long (96% over 26bp)
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC280.12194590827925612No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG280.12194590827925612No Hit
ACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCGGGGAAACG280.12194590827925612No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC280.12194590827925612Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATGGGCCGTGGCGGCGACGACCCATTCGAACGT280.12194590827925612No Hit
GTTAACAAGACGGCGCAGGTTGATGCTTCCAATAAACATGATCTTTCTGC280.12194590827925612No Hit
GGCGCAAATAGCGCTGAATCGCTTCTTTAAAGGCTGCAGCGTCGTCCTCA280.12194590827925612No Hit
GGATTGGCAACAGCAATTGAAGTGAAGATGATTGACCGCACGCAATTGAT280.12194590827925612No Hit
GCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATA280.12194590827925612No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA280.12194590827925612No Hit
ACACACAGCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGA280.12194590827925612No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC270.11759069726928269No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA270.11759069726928269No Hit
GTATCAACGCAGAGTACATGGGAACTTGCGCCAATTATCGAGCTGGTAGA270.11759069726928269No Hit
GTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGT270.11759069726928269No Hit
TTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGCCTACCAGTACT270.11759069726928269No Hit
ATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAAC260.11323548625930925No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG260.11323548625930925No Hit
GTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTT260.11323548625930925No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT260.11323548625930925No Hit
GCCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGAT260.11323548625930925No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG250.10888027524933583No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA250.10888027524933583No Hit
GAAATCAACATGGTTCAACCGCCTGACGAGTGCTGACAGCTATGAAGAAG250.10888027524933583No Hit
CAAATTGACCTCCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGAT250.10888027524933583No Hit
GTATAGGGACGCCCCCTGCTCGCGTTACTGCCAAGCGAGCGTGGTGTGGT250.10888027524933583No Hit
CTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTC240.10452506423936239No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT240.10452506423936239No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG240.10452506423936239No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT240.10452506423936239No Hit
CGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCT240.10452506423936239No Hit
GAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCC230.10016985322938896No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG230.10016985322938896No Hit
GTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTT230.10016985322938896No Hit
CCTTTTTGCTAGCATCTCGCACGGCAAGAGCGATTGCCGAGCTTAGAGCG230.10016985322938896No Hit
TGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGATCCCGG230.10016985322938896No Hit
ACGCAGAGTACATGGGAGATATTGAGGCTGCTTCGTGTCGGCACGAAGTG230.10016985322938896No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG230.10016985322938896No Hit
ATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAAATG230.10016985322938896No Hit
GGTTGATGCTTCCAATAAACATGATCTTTCTGCTCCGCTGAGAAGTACAC230.10016985322938896No Hit
ATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTAGCTCGAG230.10016985322938896No Hit
CTCTAGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGT230.10016985322938896No Hit
GATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAA230.10016985322938896No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)