FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length146
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAGGAGCATATGTGTTTCAAAAACCTAACTGTTTCTCTTTTCCTTTCTT24.444444444444445No Hit
ACATAAATGTCTTCTTTTGAGAAGTGTCTGTTCATATCCTTCGCCCACTT24.444444444444445No Hit
ATCTGGCCGCCTCAGCCTCCCAAAGTGCTGGTATTACAGGCATGAGCCAC24.444444444444445No Hit
GGGTTTCGCTCTGTCACCCAGGCTGGAGTGCAATGGCGCGATCTCGGCTC12.2222222222222223No Hit
GGATTGTAGTGGACTGGAGTGGAGCAAAATGGAGTGAAGTAGAGTGGAAG12.2222222222222223No Hit
GGTTCCCTCACGGCGCGCCTCGACGTGCGGCTCGATCGCCCACGTCACCG12.2222222222222223No Hit
ATGCTCACTCGTGTCTTCCACTGTGCATCCGTAATACCGCTGGCGAGCGT12.2222222222222223No Hit
CCTTTATTGGAACCCAAGAACGAGTAAACATAGCATTTTCAAACATCCCG12.2222222222222223No Hit
TTCTTGCAGGCCGCATCTCCCAGGACAGGGCCGAGATTTCCGCCAGTGCT12.2222222222222223No Hit
CATCAGGAATGTGTGAGATCTTGAGCAAAGCACCAAATAAACAGCCAACA12.2222222222222223No Hit
TAACCAGGATCCATACTGCCTGCCCCGGCTTCATGGTTTATGAATGTTTC12.2222222222222223No Hit
CATGTCCTTTGTAGGGACATGGATGAAGCTGGAAACCATCATTCTCAGCA12.2222222222222223No Hit
TTCCTTTTGTTGTTGACATTGTTGAGACAGGGTCTCACTTTGTCACCCAG12.2222222222222223No Hit
CTAGAAGCCCCTAGGCTTCCGCCCCCACCCCTACTCCCATGTAATCAAGA12.2222222222222223No Hit
CTCCATCTGTGCCAAAATGCTGGATCCTCCCCCCAGCGAGGATGAGCTCA12.2222222222222223No Hit
GAACGCGGGGATGGCCACGCCCCAGTCGGTCCAGTCGATGTTGACCACGT12.2222222222222223No Hit
ATGTATGAATGCGAGTGAAAGATAATCGCCAAGAGCACTGGGTTATAATA12.2222222222222223No Hit
GTGCAAGCTCACCCTGGCTTTATTTGGGGCATAATTACTAGAGACCAGCC12.2222222222222223No Hit
GACGTAGGCCACCGTGAGCCTCAGCCGCTGCTGCAGCGCGCGGATCTCCT12.2222222222222223No Hit
ACATCCTCATCACCCAATTTAAGGCTGTGTTTGCTCTTTCGGATCTTGCT12.2222222222222223No Hit
CATCAACACAAGGTGGTCTGGTCGTACCGGAACTATATGCGTTAAGCAAT12.2222222222222223No Hit
AGATGAAGGTGGAGAGTAATAGAGATGGATGGAATTTACAACAGCAAAGA12.2222222222222223No Hit
TCTTTGGGATTTTGGTTCCACAGTCCCACGTGTATTTAGTTAGCCACATC12.2222222222222223No Hit
GTCTTTAGCGGCCTTTTTGGAGCTTGAGCGCAAGCTAAAGAAGAAGTAGG12.2222222222222223No Hit
GCGCGCGACTGGGCACTGACGATCGACGAGCAGATCGTGCTGCTCGGGTC12.2222222222222223No Hit
CTGACACGACGGTCTGCCACAGCTGAACCGCTGTCGAACCTGCCATAGCT12.2222222222222223No Hit
GCCTTGAGAGGGTTCGGTCAGAAATGTGCCCTGTTATGACCTGACTGCTA12.2222222222222223No Hit
GAGCTAAAGGAGCATGTTCTAACCCAATGCAAGGAAGCTAAGAACCTTGA12.2222222222222223No Hit
ACCCTGCCCAGAGTGATTTCTACTTCTTAAATATCTCTTGTTTAGATCAA12.2222222222222223No Hit
CCATGGAAGAGTGTCTGGGGTCAGTGGCTGACAGGAGTGAACGAGGAAAC12.2222222222222223No Hit
CCTAAGACCATCCTGGCCCACCACACCCCCATCTTGTCCCTATGAAAACC12.2222222222222223No Hit
GGGTGTTTCAGTTCTAGCTAGTGGATTGCTTAATCATCAACATTCAGCTT12.2222222222222223No Hit
ACCCGGTCGTTCGCGAAGCGGCGCATCAGGTCGTCCTCGAGGGACAGGTA12.2222222222222223No Hit
TGGAGATACCATCCTTTTGTGATAATTGGTATTCTCATGTTAATCCTATG12.2222222222222223No Hit
GACGCACGCGCCGAGCTGGCGGGACTGCCGCCGGCCGGGCGCGGCCGCAA12.2222222222222223No Hit
TCCCCGTGCATGGGCTGCTGAGAAGGCACTTTTATCCTTGGGAGGACCCA12.2222222222222223No Hit
CCTTAGTATCTCACAAGCATATCAAACTTAAAAAGTCCCAAATTGAAGTC12.2222222222222223No Hit
AGGCTGGAGTGCAGTGGTGCAACCTCAGCCTTCCAAGTAGCTGGGATTAT12.2222222222222223No Hit
GTCCATGACCCCGCGATCCTCATCGCCGACGAGCCGACCGGCAACCTGGA12.2222222222222223No Hit
GGAGAATTTTTAAACATCTTTTCTTTCCTCCAGATTTTCGTGGGAGATGG12.2222222222222223No Hit
CACACACACAGAGAGAGAACGAGCTCAGTGAATTGTCCAGGTGACACACT12.2222222222222223No Hit
GAGCATGGATTCTTCTGCAGTTGAACTTTCAGATGAGACCACAGCCCTGG12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)