FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGGAGCTGTGCATCCAACACTTAGTGATCCCTTGCTGAGTGCTGCTG24.444444444444445No Hit
GGTTTTGCTAGTTTCATGATAAGCTTGTCCAAGAACTTAGTTTAGTCTTA24.444444444444445No Hit
CTGCACAGCAAAAGAAACTACCATGAGAGTGAACAGGCAACCTACAGAAT24.444444444444445No Hit
CATATGTTGGCTGTTTATTTGGTGCTTTGCTCAAGATCTCACACATTCCT12.2222222222222223No Hit
CCCTAAAGAGCCTGCAGTTTCCTTTTATATTCTTTGGGGGAAGGAAAGGC12.2222222222222223No Hit
CTCTTACTGGGGGCATTTAGGACTCTGTGCTGCCCTCTAGAGCATTTGTT12.2222222222222223No Hit
GCCAGGTCGTCTTCCAGCTGGGCGGTGACCGCAACCTTGCTCTCGATGAA12.2222222222222223No Hit
GCAAAGCGAGGCCCTGCGAGCCTTCTTGGAGCGGAACCCGTACCCGGGCA12.2222222222222223No Hit
GGTCGTGGGCCATTGTTTTGATTCGATCGGCATCGTCACGCCCAGCCACC12.2222222222222223No Hit
GCGGCACGGGTTTCGGCCGGAAGCGTGTCGCGGCGGGCCACTTCGGCTCG12.2222222222222223No Hit
GCCTTACCCTTTTGGATGTATTTGGATTCCATAAAGGTGAGTACAATCAA12.2222222222222223No Hit
CCTCTACCGCTCTCCCAGGAGAGTTGAGTTCCTGAGAAAAGCAATCTGGC12.2222222222222223No Hit
GCGCATGAGCGGCGTGAAGAAGGAGGAGGCCGCGGCGCGGGCGCGCGAGG12.2222222222222223No Hit
GCATAAGATAACTGGTAATGAATTGTGATGGATCAGAGATGATGAGGAAC12.2222222222222223No Hit
CTCCTGTAATCATAATCCCAGCTACTTGGAAGGCTGAGGTTGCACCACTG12.2222222222222223No Hit
CACTATAGCCTCAAACTCCTGGGCTCAAGGGGAGAATCACTTGAGCCCAG12.2222222222222223No Hit
GGCCCGTCTTGTAGCTGGTGCGACGAATTACTCGGCTCGCAGGTATGGCG12.2222222222222223No Hit
ATAGAACGGTATCTTGTATTTCTTCTGTCCAGGTATACTTATAAATGCCT12.2222222222222223No Hit
CCGAGGGTCACCTGGAGACTGCAGATTGACGAACCTTCAGCTCCCGCAGT12.2222222222222223No Hit
CACTCAAGGACTGCCTTTTACAGACCTCTTCAGTGATTTGTTCCCTTCTA12.2222222222222223No Hit
GGACGAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGTAACTGA12.2222222222222223No Hit
ACAACATATGATTTCCTCCATAAGATACACACTTGAGAAGATGTGGGTAC12.2222222222222223No Hit
CTGTTTCCAAAACCCCAACACATGGACACAATGACCCTAACTGCAGTGAT12.2222222222222223No Hit
GCTTTAGGGTTTCTCACAAGGCTGCAATCAAGTTGTCAGCCAGGGCTGTG12.2222222222222223No Hit
GTTATACGGATTTTAGGAGCTTTCTCTTTATAGCTCGTTTCTCTCTGGTT12.2222222222222223No Hit
ATGTAATTGTGGTTCTTTGAGGATCAGTGTCGTTACCTACTTCCGCATAA12.2222222222222223No Hit
GCCTACTGACTTGGCCCCGGGCCCAGGCGGTTGTGGGGAAGTGTCCCCCA12.2222222222222223No Hit
GTCCTGCCCCTCTCCCAGGATTCTTCTAAAGATCAAGGGAGAAGGGAGGG12.2222222222222223No Hit
GCATAGGAGGGGGGCCTCAGTGCCCCAATGGACGAAAGTCCCTTCCCAGC12.2222222222222223No Hit
GAGCATGTCCCAGACGTACCTTATCTACAGCCAGCAATTTGTCACCTTCA12.2222222222222223No Hit
CACCACGAAGGAGGCCAAACCCTGACAGCCAGAGACCAGGGGCCGGGGCT12.2222222222222223No Hit
CACATAGTCCCATATTTCTTGGAGGCTTTGTTCATTCCTTTTCATTGTTT12.2222222222222223No Hit
GCGTCGGCTCGTGGCATCGATGACGCGACCGTCGCCGCAAGCCTTATGGG12.2222222222222223No Hit
ATCAGCGTCGAGGTGTCCGAGATGCTGTCGGATCTGCTGAAGCGGCGCGG12.2222222222222223No Hit
GATGTGGCTAACTAAATACACGTGGGACTGTGGAACCAAAATCCCAAAGA12.2222222222222223No Hit
ATTGTGGATAAGCTTTGTGATTTGCTGCTGGATTCGGTTTGCTAGTATTT12.2222222222222223No Hit
TGCGATAGTTTGCTGAGAATGATGGTTTCCAGCTTCATCCATGTCCCTAC12.2222222222222223No Hit
CAACTCCCTAGAGTAGAAAGTCATTGGAGTGTCAACTTTTTGACCTCAGG12.2222222222222223No Hit
ATTTTCCCCTGGAGTCGGACCACTTAGTGGCCCAGGCTCTCCTCCAACTG12.2222222222222223No Hit
AAGCAGTACCTCGACGTACTCAAGCCTCTGCGTGAGGAGCTGGGTGTGCC12.2222222222222223No Hit
CCCTCGGTGCGGTGGCCGGCGGCACGGGTTCCGTGTCCTCCAACCAGATC12.2222222222222223No Hit
CTTCGTACTGACTGGGCCGCTGGCCGCCACCGACGAGCATCCGGCCACCG12.2222222222222223No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)