FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003647220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003647220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length146
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTTTGCCTTGTGGCCCAAACGAGTCTCCTTCACCGTCCCCGTCCCCG38.571428571428571No Hit
ATGTTGGCCAGGCTGGTCTCAAACTCCTGACCTCAAGTGATCTGCCTGCC25.714285714285714No Hit
CCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCC25.714285714285714No Hit
GAACAGGACAATGTGAGAGATATACTTCAGGAGGCCTGAAAGCTGGTCAT25.714285714285714No Hit
GTTACCAGGTCAGTAGCTTGAAGGAGAATAATCCTCAGTGCCCTTATTAC25.714285714285714No Hit
GTCTTTTTCTGGCAAACTCTCAATGAAATCAGCCTCAGCAGACGTTAATG12.857142857142857No Hit
GAGGAGCGTCTGCGCCCGGCCGCCATCCCATCTAGGAAGTGAGGAGCGCC12.857142857142857No Hit
CTATACCACTCATTGCCCTAGGAACAGTATCTTGGGGGAAGCTCTGGACA12.857142857142857No Hit
TGGTTGAGCAGCGTGCAAATCGAATTTCCACCATTGAGACACATCCTACT12.857142857142857No Hit
GGGAGATTCTATCTTCCCAAGAGAATCTCATCCAACCACTTTTGTTTTTT12.857142857142857No Hit
GCTGCCGATGCCATCCGTTCTCGCTTATCTCTGACCAGCGTCGACATGGG12.857142857142857No Hit
GACTTCAGCAATCTTCCGGCCCTGACCATAAAAGGACCAGCAGCAGATTT12.857142857142857No Hit
GTTATAAAGAAACAGCACTATCCCACGAACCAAAATGTTTCATTACGTTT12.857142857142857No Hit
GAACCACCACAGTACCGGGGTCCCGGTGGCAGAGATGACGCGCAAGCAAG12.857142857142857No Hit
GGTATACCAACATGAGAGATTTAATTTTATTACATAGTGATGTATTTCAT12.857142857142857No Hit
GAAGAAAGCAGCGTGCATCCGTTCGCCCTGCACGCGCAGCATCATGACCG12.857142857142857No Hit
CATCTGGATCTTCCAGAGTTCCAAGCGTCTTCTTGGGTACAGTTGTGCTC12.857142857142857No Hit
GTTACCTGGGAGACTGCGGCAGGAGAATTGCTTGAACCTGGGAGGCGGAG12.857142857142857No Hit
GGACGAAGAAGAGGAAGAAGACCAAGGCCCACCATGCCCCAGGTAACTGA12.857142857142857No Hit
GCGGCGCCCGGCGCGTTCGTGTTTCGCCCGAAACTGCACGAGCCCGGCGT12.857142857142857No Hit
GATCACTTGAGCCCAGAAAGCGGAGGTTGCAGTGAGCCGATTGCATCACT12.857142857142857No Hit
GGCTCGACGCGCTGGGCCTCAAGGACACCATCATCATGCATCCTGGTCCG12.857142857142857No Hit
TCAGTGGATCGTCGATTTTTTCACCGATTTTAAATGCTGATGTCGGTGCA12.857142857142857No Hit
ACCTTAACTTCCATCCTAGACTCAATGCCAGGAATCTAAATGGGCTGTAG12.857142857142857No Hit
TCGTGGTTCTTCACGCGCGCCTGGTGGCACGCAAAACACGTCTGCTGTCT12.857142857142857No Hit
GTTCAGGGTCTTCGGCCGGATCGTGCGGCCGCGGCTGGTCAGGATGTTGT12.857142857142857No Hit
ATTACCACGTACGGGTGGTCAACTCTGGCGCCCGCGCCTTGCAGGCGGTG12.857142857142857No Hit
GGTAGGACCGCAGCCTCCTGAGACCCCTCTCGGTCCTACAGTTCTACCGT12.857142857142857No Hit
TTACTATACACAACGATTTTGAGTTCATGCGCCCGTTCAAAAATTCCAGT12.857142857142857No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)